|
|
Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
26/09/2023 |
Data da última atualização: |
27/09/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
TAVARES, J. V.; OLIVEIRA, R. S.; MENCUCCINI, M.; SIGNORI-MÜLLER , C.; PEREIRA, L.; DINIZ, F. C.; GILPIN, M.; ZEVALLOS, M. J. M.; SALAS YUPAYCCANA, C. A.; ACOSTA, M.; PÉREZ MULLISACA, F. M.; BARROS, F. de V.; BITTENCOURT, P.; JANCOSKI, H.; SCALON, M. C.; MARIMON, B. S.; MENOR, I. O.; MARIMON JUNIOR, B. H.; FANCOURT, M.; CHAMBERS-OSTLER, A.; ESQUIVEL-MUELBERT, A.; ROWLAND, L.; MEIR, P.; COSTA, A. C. L. da; NINA, A.; SANCHEZ, J. M. B.; TINTAYA, J. S.; CHINO, R. S. C.; BACA, J.; FERNANDES, L.; CUMAPA, E. R. M.; SANTOS, J. A. R.; TEIXEIRA, R.; TELLO, L.; UGARTECHE, M. T. M.; CUELLAR, G. A.; MARTINEZ, F.; ARAUJO-MURAKAMI, A.; ALMEIDA, E.; CRUZ, W. J. A. da; PASQUEL, J. del A.; ARAGÃO, L.; BAKER, T. R.; CAMARGO, P. B. de; BRIENEN, R.; CASTRO, W.; RIBEIRO, S. C.; SOUZA, F. C. de; COSIO, E. G.; CARDOZO, N. D.; SILVA, R. da C.; DISNEY, M.; ESPEJO, J. S.; FELDPAUSCH, T. R.; FERREIRA, L.; GIACOMIN, L.; HIGUCHI, N.; HIROTA, M.; HONORIO, E.; HUASCO, W. H.; LEWIS, S.; FLORES LLAMPAZO, G.; MALHI, Y.; MONTEAGUDO MENDOZA, A.; MORANDI, P.; MOSCOSO, V. C.; MUSCARELLA, R.; PENHA, D.; ROCHA, M. C.; RODRIGUES, G.; RUSCHEL, A. R.; SALINAS, N.; SCHLICKMANN, M.; SILVEIRA, M.; TALBOT, J.; VÁSQUEZ, R.; VEDOVATO, L.; VIEIRA, S. A.; PHILLIPS, O. L.; GLOOR, E.; GALBRAITH, D. R. |
Afiliação: |
JULIA VALENTIM TAVARES, UNIVERSITY OF LEEDS; RAFAEL S. OLIVEIRA, UNIVERSIDADE DE CAMPINAS; MAURIZIO MENCUCCINI, CENTRO DE INVESTIGACIÓN ECOLÓGICA Y APLICACIONES FORESTALES; CAROLINE SIGNORI-MÜLLER, UNIVERSITY OF LEEDS; LUCIANO PEREIRA, UNIVERSIDADE DE CAMPINAS; FRANCISCO CARVALHO DINIZ, UNIVERSITY OF LEEDS; MARTIN GILPIN, UNIVERSITY OF LEEDS; MANUEL J. MARCA ZEVALLOS, UNIVERSIDAD NACIONAL DE SAN ANTONIO ABAD DEL CUSCO; CARLOS A. SALAS YUPAYCCANA, UNIVERSIDAD NACIONAL DE SAN ANTONIO ABAD DEL CUSCO; MARTIN ACOSTA, UNIVERSIDADE FEDERAL DO ACRE; FLOR M. PÉREZ MULLISACA, UNIVERSIDAD NACIONAL DE SAN ANTONIO ABAD DEL CUSCO; FERNANDA DE V. BARROS, UNIVERSITY OF EXETER; PAULO BITTENCOURT, UNIVERSIDADE DE CAMPINAS; HALINA JANCOSKI, UNIVERSIDADE DO ESTADO DE MATO GROSSO; MARINA CORRÊA SCALON, UNIVERSIDADE DO ESTADO DE MATO GROSSO; BEATRIZ S. MARIMON, UNIVERSIDADE DO ESTADO DE MATO GROSSO; IMMA OLIVERAS MENOR, UNIVERSITY OF OXFORD; BEN HUR MARIMON JUNIOR, UNIVERSIDADE DO ESTADO DE MATO GROSSO; MAX FANCOURT, UNIVERSITY OF LEEDS; ALEXANDER CHAMBERS-OSTLER, UNIVERSITY OF LEEDS; ADRIANE ESQUIVEL-MUELBERT, UNIVERSITY OF BIRMINGHAM; LUCY ROWLAND, UNIVERSITY OF EXETER; PATRICK MEIR, UNIVERSITY OF EDINBURGH; ANTONIO CARLOS LOLA DA COSTA, UNIVERSIDADE FEDERAL DO PARÁ; ALEX NINA, PONTIFICIA UNIVERSIDAD CATÓLICA DEL PERÚ; JESUS M. B. SANCHEZ, UNIVERSIDAD NACIONAL DE SAN ANTONIO ABAD DEL CUSCO; JOSE S. TINTAYA, UNIVERSIDAD NACIONAL DE SAN ANTONIO ABAD DEL CUSCO; RUDI S. C. CHINO, PONTIFICIA UNIVERSIDAD CATÓLICA DEL PERÚ; JEAN BACA, UNIVERSIDAD NACIONAL DE LA AMAZONIA PERUANA; LETICIA FERNANDES, UNIVERSIDAD NACIONAL DE SAN ANTONIO ABAD DEL CUSCO; EDWIN R. M. CUMAPA, UNIVERSIDADE FEDERAL DO PARÁ; JOÃO ANTÔNIO R. SANTOS, UNIVERSIDAD NACIONAL DE SAN ANTONIO ABAD DEL CUSCO; RENATA TEIXEIRA, UNIVERSIDAD NACIONAL DE SAN ANTONIO ABAD DEL CUSCO; LIGIA TELLO, UNIVERSIDAD NACIONAL DE LA AMAZONIA PERUANA; MAIRA T. M. UGARTECHE, MUSEO DE HISTORIA NATURAL NOEL KEMPFF MERCADO; GINA A. CUELLAR, MUSEO DE HISTORIA NATURAL NOEL KEMPFF MERCADO; FRANKLIN MARTINEZ, MUSEO DE HISTORIA NATURAL NOEL KEMPFF MERCADO; ALEJANDRO ARAUJO-MURAKAMI, MUSEO DE HISTORIA NATURAL NOEL KEMPFF MERCADO; EVERTON ALMEIDA, UNIVERSIDADE FEDERAL DO OESTE DO PARÁ; WESLEY JONATAR ALVES DA CRUZ, UNIVERSIDADE DO ESTADO DE MATO GROSSO; JHON DEL AGUILA PASQUEL, UNIVERSIDAD NACIONAL DE LA AMAZONIA PERUANA; LUIS ARAGÃO, INSTITUTO NACIONAL DE PESQUISAS ESPACIAIS; TIMOTHY R. BAKER, UNIVERSITY OF LEEDS; PLINIO BARBOSA DE CAMARGO, UNIVERSIDADE DE SĀO PAULO; ROEL BRIENEN, UNIVERSITY OF LEEDS; WENDESON CASTRO, UNIVERSIDADE FEDERAL DO ACRE; SABINA CERRUTO RIBEIRO, UNIVERSIDADE FEDERAL DO ACRE; FERNANDA COELHO DE SOUZA, UNIVERSIDADE DE BRASÍLIA; ERIC G. COSIO, PONTIFICIA UNIVERSIDAD CATÓLICA DEL PERÚ; NALLARET DAVILA CARDOZO, INSTITUTO DE INVESTIGACIONES DE LA AMAZONIA PERUANA; RICHARLLY DA COSTA SILVA, UNIVERSIDADE FEDERAL DO ACRE; MATHIAS DISNEY, UNIVERSITY COLLEGE LONDON; JAVIER SILVA ESPEJO, UNIVERSIDAD NACIONAL DE SAN ANTONIO ABAD DEL CUSCO; TED R. FELDPAUSCH, UNIVERSITY OF EXETER; LEANDRO FERREIRA, MUSEU PARAENSE EMÍLIO GOELDI; LEANDRO GIACOMIN, UNIVERSIDADE FEDERAL DA PARAÍBA; NIRO HIGUCHI, INSTITUTO NACIONAL DE PESQUISAS DA AMAZÔNIA; MARINA HIROTA, UNIVERSIDADE DE CAMPINAS; EURIDICE HONORIO, INSTITUTO DE INVESTIGACIONES DE LA AMAZONIA PERUANA; WALTER HUARACA HUASCO, UNIVERSITY OF OXFORD; SIMON LEWIS, UNIVERSITY OF LEEDS; GERARDO FLORES LLAMPAZO, INSTITUTO DE INVESTIGACIONES DE LA AMAZONIA PERUANA; YADVINDER MALHI, UNIVERSITY OF OXFORD; ABEL MONTEAGUDO MENDOZA, UNIVERSIDAD NACIONAL DE SAN ANTONIO ABAD DEL CUSCO; PAULO MORANDI, UNIVERSIDADE DO ESTADO DE MATO GROSSO; VICTOR CHAMA MOSCOSO, UNIVERSIDAD NACIONAL DE SAN ANTONIO ABAD DEL CUSCO; ROBERT MUSCARELLA, UPPSALA UNIVERSITY; DELIANE PENHA, UNIVERSIDADE FEDERAL DO OESTE DO PARÁ; MAYDA CECÍLIA ROCHA, UNIVERSIDADE FEDERAL DO OESTE DO PARÁ; GLEICY RODRIGUES, INSTITUTO NACIONAL DE PESQUISAS DA AMAZÔNIA; ADEMIR ROBERTO RUSCHEL, CPATU; NORMA SALINAS, UNIVERSITY OF OXFORD; MONIQUE SCHLICKMANN, UNIVERSIDADE FEDERAL DO OESTE DO PARÁ; MARCOS SILVEIRA, UNIVERSIDADE FEDERAL DO ACRE; JOEY TALBOT, UNIVERSITY OF LEEDS; RODOLFO VÁSQUEZ, JARDÍN BOTÁNICO DE MISSOURI; LAURA VEDOVATO, UNIVERSITY OF EXETER; SIMONE APARECIDA VIEIRA, UNIVERSIDADE ESTADUAL DE CAMPINAS; OLIVER L. PHILLIPS, UNIVERSITY OF LEEDS; EMANUEL GLOOR, UNIVERSITY OF LEEDS; DAVID R. GALBRAITH, UNIVERSITY OF LEEDS. |
Título: |
Basin-wide variation in tree hydraulic safety margins predicts the carbon balance of Amazon forests. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Nature, v. 617, p. 111-117, 2023. |
DOI: |
https://doi.org/10.1038/s41586-023-05971-3 |
Idioma: |
Inglês |
Conteúdo: |
Tropical forests face increasing climate risk1,2, yet our ability to predict their response to climate change is limited by poor understanding of their resistance to water stress. Although xylem embolism resistance thresholds (for example, Ψ50) and hydraulic safety margins (for example, HSM50) are important predictors of drought-induced mortality risk3,4,5, little is known about how these vary across Earth?s largest tropical forest. Here, we present a pan-Amazon, fully standardized hydraulic traits dataset and use it to assess regional variation in drought sensitivity and hydraulic trait ability to predict species distributions and long-term forest biomass accumulation. Parameters Ψ50 and HSM50 vary markedly across the Amazon and are related to average long-term rainfall characteristics. Both Ψ50 and HSM50 influence the biogeographical distribution of Amazon tree species. However, HSM50 was the only significant predictor of observed decadal-scale changes in forest biomass. Old-growth forests with wide HSM50 are gaining more biomass than are low HSM50 forests. We propose that this may be associated with a growth?mortality trade-off whereby trees in forests consisting of fast-growing species take greater hydraulic risks and face greater mortality risk. Moreover, in regions of more pronounced climatic change, we find evidence that forests are losing biomass, suggesting that species in these regions may be operating beyond their hydraulic limits. Continued climate change is likely to further reduce HSM50 in the Amazon6,7, with strong implications for the Amazon carbon sink. MenosTropical forests face increasing climate risk1,2, yet our ability to predict their response to climate change is limited by poor understanding of their resistance to water stress. Although xylem embolism resistance thresholds (for example, Ψ50) and hydraulic safety margins (for example, HSM50) are important predictors of drought-induced mortality risk3,4,5, little is known about how these vary across Earth?s largest tropical forest. Here, we present a pan-Amazon, fully standardized hydraulic traits dataset and use it to assess regional variation in drought sensitivity and hydraulic trait ability to predict species distributions and long-term forest biomass accumulation. Parameters Ψ50 and HSM50 vary markedly across the Amazon and are related to average long-term rainfall characteristics. Both Ψ50 and HSM50 influence the biogeographical distribution of Amazon tree species. However, HSM50 was the only significant predictor of observed decadal-scale changes in forest biomass. Old-growth forests with wide HSM50 are gaining more biomass than are low HSM50 forests. We propose that this may be associated with a growth?mortality trade-off whereby trees in forests consisting of fast-growing species take greater hydraulic risks and face greater mortality risk. Moreover, in regions of more pronounced climatic change, we find evidence that forests are losing biomass, suggesting that species in these regions may be operating beyond their hydraulic limits. Continued climate... Mostrar Tudo |
Palavras-Chave: |
Ecofisiologia; Ecologia tropical; Tropical ecology. |
Thesagro: |
Biodiversidade; Ecologia; Ecologia Florestal. |
Thesaurus Nal: |
Biodiversity; Ecophysiology; Forest ecology. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156907/1/Basin-wide-variation.pdf
|
Marc: |
LEADER 04791naa a2201201 a 4500 001 2156907 005 2023-09-27 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1038/s41586-023-05971-3$2DOI 100 1 $aTAVARES, J. V. 245 $aBasin-wide variation in tree hydraulic safety margins predicts the carbon balance of Amazon forests.$h[electronic resource] 260 $c2023 520 $aTropical forests face increasing climate risk1,2, yet our ability to predict their response to climate change is limited by poor understanding of their resistance to water stress. Although xylem embolism resistance thresholds (for example, Ψ50) and hydraulic safety margins (for example, HSM50) are important predictors of drought-induced mortality risk3,4,5, little is known about how these vary across Earth?s largest tropical forest. Here, we present a pan-Amazon, fully standardized hydraulic traits dataset and use it to assess regional variation in drought sensitivity and hydraulic trait ability to predict species distributions and long-term forest biomass accumulation. Parameters Ψ50 and HSM50 vary markedly across the Amazon and are related to average long-term rainfall characteristics. Both Ψ50 and HSM50 influence the biogeographical distribution of Amazon tree species. However, HSM50 was the only significant predictor of observed decadal-scale changes in forest biomass. Old-growth forests with wide HSM50 are gaining more biomass than are low HSM50 forests. We propose that this may be associated with a growth?mortality trade-off whereby trees in forests consisting of fast-growing species take greater hydraulic risks and face greater mortality risk. Moreover, in regions of more pronounced climatic change, we find evidence that forests are losing biomass, suggesting that species in these regions may be operating beyond their hydraulic limits. Continued climate change is likely to further reduce HSM50 in the Amazon6,7, with strong implications for the Amazon carbon sink. 650 $aBiodiversity 650 $aEcophysiology 650 $aForest ecology 650 $aBiodiversidade 650 $aEcologia 650 $aEcologia Florestal 653 $aEcofisiologia 653 $aEcologia tropical 653 $aTropical ecology 700 1 $aOLIVEIRA, R. S. 700 1 $aMENCUCCINI, M. 700 1 $aSIGNORI-MÜLLER , C. 700 1 $aPEREIRA, L. 700 1 $aDINIZ, F. C. 700 1 $aGILPIN, M. 700 1 $aZEVALLOS, M. J. M. 700 1 $aSALAS YUPAYCCANA, C. A. 700 1 $aACOSTA, M. 700 1 $aPÉREZ MULLISACA, F. M. 700 1 $aBARROS, F. de V. 700 1 $aBITTENCOURT, P. 700 1 $aJANCOSKI, H. 700 1 $aSCALON, M. C. 700 1 $aMARIMON, B. S. 700 1 $aMENOR, I. O. 700 1 $aMARIMON JUNIOR, B. H. 700 1 $aFANCOURT, M. 700 1 $aCHAMBERS-OSTLER, A. 700 1 $aESQUIVEL-MUELBERT, A. 700 1 $aROWLAND, L. 700 1 $aMEIR, P. 700 1 $aCOSTA, A. C. L. da 700 1 $aNINA, A. 700 1 $aSANCHEZ, J. M. B. 700 1 $aTINTAYA, J. S. 700 1 $aCHINO, R. S. C. 700 1 $aBACA, J. 700 1 $aFERNANDES, L. 700 1 $aCUMAPA, E. R. M. 700 1 $aSANTOS, J. A. R. 700 1 $aTEIXEIRA, R. 700 1 $aTELLO, L. 700 1 $aUGARTECHE, M. T. M. 700 1 $aCUELLAR, G. A. 700 1 $aMARTINEZ, F. 700 1 $aARAUJO-MURAKAMI, A. 700 1 $aALMEIDA, E. 700 1 $aCRUZ, W. J. A. da 700 1 $aPASQUEL, J. del A. 700 1 $aARAGÃO, L. 700 1 $aBAKER, T. R. 700 1 $aCAMARGO, P. B. de 700 1 $aBRIENEN, R. 700 1 $aCASTRO, W. 700 1 $aRIBEIRO, S. C. 700 1 $aSOUZA, F. C. de 700 1 $aCOSIO, E. G. 700 1 $aCARDOZO, N. D. 700 1 $aSILVA, R. da C. 700 1 $aDISNEY, M. 700 1 $aESPEJO, J. S. 700 1 $aFELDPAUSCH, T. R. 700 1 $aFERREIRA, L. 700 1 $aGIACOMIN, L. 700 1 $aHIGUCHI, N. 700 1 $aHIROTA, M. 700 1 $aHONORIO, E. 700 1 $aHUASCO, W. H. 700 1 $aLEWIS, S. 700 1 $aFLORES LLAMPAZO, G. 700 1 $aMALHI, Y. 700 1 $aMONTEAGUDO MENDOZA, A. 700 1 $aMORANDI, P. 700 1 $aMOSCOSO, V. C. 700 1 $aMUSCARELLA, R. 700 1 $aPENHA, D. 700 1 $aROCHA, M. C. 700 1 $aRODRIGUES, G. 700 1 $aRUSCHEL, A. R. 700 1 $aSALINAS, N. 700 1 $aSCHLICKMANN, M. 700 1 $aSILVEIRA, M. 700 1 $aTALBOT, J. 700 1 $aVÁSQUEZ, R. 700 1 $aVEDOVATO, L. 700 1 $aVIEIRA, S. A. 700 1 $aPHILLIPS, O. L. 700 1 $aGLOOR, E. 700 1 $aGALBRAITH, D. R. 773 $tNature$gv. 617, p. 111-117, 2023.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Amazônia Oriental (CPATU) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
07/02/2017 |
Data da última atualização: |
30/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
PAES, V. M.; VIEIRA, L. A.; CORREIA, H. H. V.; SA, N. A. R.; MOURA, A. A. A.; SALES, A. D.; RODRIGUES, A. P. R.; MAGALHÃES-PADILHA, D. M.; SANTOS, F. W.; APGAR, G. A.; CAMPELLO, C. C.; CAMARGO, L. S. de A.; FIGUEIREDO, J. R. |
Afiliação: |
V. M. Paes, LAMOFOPA - CE; L. A. Vieira, LAMOFOPA - CE; H. H. V. Correia, LAMOFOPA - CE; N. A. R. Sa, LAMOFOPA - CE; A. A. A. Moura, Universidade Federal do Ceará; A. D. Sales, LAMOFOPA - CE; A. P. R. Rodrigues, LAMOFOPA - CE; D. M. Magalhães-Padilha, Universidade Potiguar; F. W. Santos, Universidade Estadual do Pampa; G. A. Apgar, Southern Illinois University, Carbondale, Illinois, USA; C. C. Campello, LAMOFOPA - CE; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; J. R. Figueiredo, LAMOFOPA - CE. |
Título: |
Effect of heat stress on the survival and development of in vitro cultured bovine preantral follicles and on in vitro maturation of cumulus-oocyte complex. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Theriogenology, n. 86, p. 994-1003, 2016. |
Idioma: |
Português |
Conteúdo: |
Abstract The deleterious effect of heat stress (HS) on competence of oocytes from antral follicles is well recognized, but there is a lack of data regarding its impact on the viability and growth of preantral follicles. In this study, we used in vitro preantral follicle cultures to investigate the effects of HS on the following parameters: survival and development of primordial follicles after in vitro culture of ovarian fragments (experiment I); growth and antrum formation of isolated advanced secondary follicles (experiment II); and maturation rates after in vitro maturation (IVM) of cumulus?oocyte complexes (COCs) from antral follicles (>2?6 mm) grown in vivo (experiment III). Furthermore, the following end points were evaluated in all experiments: follicle/oocyte survival, reactive oxygen species (ROS), estradiol (E2) and progesterone (P4) production, as well asmRNA expression for select genes related to stress (HSP70) and apoptosis (MCL1 and BAX). In all experiments, HS consisted of exposing the structures (ovarian fragments, isolated preantral follicles and COCs) to 41 C for 12 hours and then to 38.5 C until the end of the culture (7 days for experiments I and II and 24 hours for experiment III). The temperature for the control group was held at 38.5 C for the entire culture period. Heat stress increased (P < 0.05) the percentage of developing follicles (intermediate, primary, and secondary follicles) at 12 hours and increased levels of ROS at all evaluated time points (12, 24 hours, and D7), when compared to the control (experiment I). Heat stress did not affect (P > 0.05) any identified end points when preantral follicles were cultured in their isolated form (experiment II). However, in experiment III, HS decreased (P < 0.05) both the rates of metaphase II after 24 hours and E2 production at 12 hours of IVM. Moreover, HS increased (P < 0.0001) levels of P4 after IVM and ROS production at every evaluated time point, compared with the control (12 and 24 hours). In conclusion, HS caused: (1) early activation of primordial follicles; (2) an increase in ROS production by early preantral follicles enclosed in ovarian tissue and by COCs; (3) a short-term reduction of E2 production by COCs; and (4) an increase in P4 secretion from COCs. However, HS did not affectin vitro culture of advanced isolated secondary follicles. Experimental evidence indicates that preantral follicles are less sensitive to HS than COC. MenosAbstract The deleterious effect of heat stress (HS) on competence of oocytes from antral follicles is well recognized, but there is a lack of data regarding its impact on the viability and growth of preantral follicles. In this study, we used in vitro preantral follicle cultures to investigate the effects of HS on the following parameters: survival and development of primordial follicles after in vitro culture of ovarian fragments (experiment I); growth and antrum formation of isolated advanced secondary follicles (experiment II); and maturation rates after in vitro maturation (IVM) of cumulus?oocyte complexes (COCs) from antral follicles (>2?6 mm) grown in vivo (experiment III). Furthermore, the following end points were evaluated in all experiments: follicle/oocyte survival, reactive oxygen species (ROS), estradiol (E2) and progesterone (P4) production, as well asmRNA expression for select genes related to stress (HSP70) and apoptosis (MCL1 and BAX). In all experiments, HS consisted of exposing the structures (ovarian fragments, isolated preantral follicles and COCs) to 41 C for 12 hours and then to 38.5 C until the end of the culture (7 days for experiments I and II and 24 hours for experiment III). The temperature for the control group was held at 38.5 C for the entire culture period. Heat stress increased (P < 0.05) the percentage of developing follicles (intermediate, primary, and secondary follicles) at 12 hours and increased levels of ROS at all evaluated time points... Mostrar Tudo |
Palavras-Chave: |
Bovine; Oocyte; Preantral follicle. |
Thesaurus NAL: |
heat stress; in vitro culture. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03440naa a2200325 a 4500 001 2062969 005 2023-01-30 008 2016 bl uuuu u00u1 u #d 100 1 $aPAES, V. M. 245 $aEffect of heat stress on the survival and development of in vitro cultured bovine preantral follicles and on in vitro maturation of cumulus-oocyte complex.$h[electronic resource] 260 $c2016 520 $aAbstract The deleterious effect of heat stress (HS) on competence of oocytes from antral follicles is well recognized, but there is a lack of data regarding its impact on the viability and growth of preantral follicles. In this study, we used in vitro preantral follicle cultures to investigate the effects of HS on the following parameters: survival and development of primordial follicles after in vitro culture of ovarian fragments (experiment I); growth and antrum formation of isolated advanced secondary follicles (experiment II); and maturation rates after in vitro maturation (IVM) of cumulus?oocyte complexes (COCs) from antral follicles (>2?6 mm) grown in vivo (experiment III). Furthermore, the following end points were evaluated in all experiments: follicle/oocyte survival, reactive oxygen species (ROS), estradiol (E2) and progesterone (P4) production, as well asmRNA expression for select genes related to stress (HSP70) and apoptosis (MCL1 and BAX). In all experiments, HS consisted of exposing the structures (ovarian fragments, isolated preantral follicles and COCs) to 41 C for 12 hours and then to 38.5 C until the end of the culture (7 days for experiments I and II and 24 hours for experiment III). The temperature for the control group was held at 38.5 C for the entire culture period. Heat stress increased (P < 0.05) the percentage of developing follicles (intermediate, primary, and secondary follicles) at 12 hours and increased levels of ROS at all evaluated time points (12, 24 hours, and D7), when compared to the control (experiment I). Heat stress did not affect (P > 0.05) any identified end points when preantral follicles were cultured in their isolated form (experiment II). However, in experiment III, HS decreased (P < 0.05) both the rates of metaphase II after 24 hours and E2 production at 12 hours of IVM. Moreover, HS increased (P < 0.0001) levels of P4 after IVM and ROS production at every evaluated time point, compared with the control (12 and 24 hours). In conclusion, HS caused: (1) early activation of primordial follicles; (2) an increase in ROS production by early preantral follicles enclosed in ovarian tissue and by COCs; (3) a short-term reduction of E2 production by COCs; and (4) an increase in P4 secretion from COCs. However, HS did not affectin vitro culture of advanced isolated secondary follicles. Experimental evidence indicates that preantral follicles are less sensitive to HS than COC. 650 $aheat stress 650 $ain vitro culture 653 $aBovine 653 $aOocyte 653 $aPreantral follicle 700 1 $aVIEIRA, L. A. 700 1 $aCORREIA, H. H. V. 700 1 $aSA, N. A. R. 700 1 $aMOURA, A. A. A. 700 1 $aSALES, A. D. 700 1 $aRODRIGUES, A. P. R. 700 1 $aMAGALHÃES-PADILHA, D. M. 700 1 $aSANTOS, F. W. 700 1 $aAPGAR, G. A. 700 1 $aCAMPELLO, C. C. 700 1 $aCAMARGO, L. S. de A. 700 1 $aFIGUEIREDO, J. R. 773 $tTheriogenology$gn. 86, p. 994-1003, 2016.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|