Data/hora: 23/06/2024 01:31:17

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 04/05/2018
Data da última atualização: | 04/05/2018
Autoria: | OSEI, O.; ABAIDOO, R. C.; BODDEY, R. M.; ROUWS, L. F. M.
Afiliação: | OPHELIA OSEIA, Kwame Nkrumah University of Science and Technology, PMB, Kumasi, Ghana; ROBERT C. ABAIDOO, Kwame Nkrumah University of Science and Technology, PMB, Kumasi, Ghana; ROBERT MICHAEL BODDEY, CNPAB; LUC FELICIANUS MARIE ROUWS, CNPAB.
Título: | Bacteria related to Bradyrhizobium yuanmingense from Ghana are effective groundnut micro-symbionts.
Ano de publicação: | 2018
Fonte/Imprenta: | Applied Soil Ecology, v. 127, p. 41-50, 2018.
ISSN: | 0929-1393
DOI: | https://doi.org/10.1016/j.apsoil.2018.03.003
Idioma: | Inglês
Conteúdo: | were obtained, 30 of which were eventually found to nodulate groundnut plants. After testing the symbiotic potential of these isolates on groundnut on sterilized substrate, seven of them, designated as KNUST 1001?1007, were evaluated in an open field pot experiment using 15N-labelled soil. Although 15N dilution analyses did not indicate differences among treatments in the proportion of nitrogen (N) derived from the atmosphere (%Ndfa), all seven strains increased total N derived from N2 fixation by inoculated groundnut plants as compared to the non-inoculated control. Inoculation with KNUST 1002 led to total N accumulation as high as that of the groundnut reference strain 32H1. Genetic characterisation of the isolates by sequence analysis of 16S rRNA gene, 16S ? 23S rRNA intergenic transcribed spacer (ITS) region and nodC gene revealed that isolates KNUST 1003 and 1007 were related to Rhizobium tropici, a common bean symbiont. The other five isolates, including KNUST 1002 belonged to the Bradyrhizobium genus, being closely related to Bradyrhizobium yuanmingense. Therefore, this study revealed novel native Ghanaian rhizobia with potential for the development of groundnut inoculants.
Palavras-Chave: | Biological nitrogen fixation; Genetic diversity Arachis hypogaea L; Native isolates.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (40732-1) - UPC - AP - DD - 2018.00025 -  - 2018.00025 - DIS