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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
07/03/2023 |
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Data da última atualização: |
07/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SCHWAB, S.; HIRATA, E. S.; AMARAL, J. C. A.; SILVA, C. G. N.; FERREIRA, J. P.; SILVA, L. V. da; ROUWS, J. R. C.; ROUWS, L. F. M.; BALDANI, J. I.; REIS, V. M. |
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Afiliação: |
STEFAN SCHWAB, CNPAB; BASF S.A; J. C. A. AMARAL, UFRRJ; C. G. N. DA SILVA, BOLSISTA EMBRAPA AGROBIOLOGIA; J. P. FERREIRA, SOLUBIO; L. V. DA SILVA, BOLSISTA EMBRAPA AGROBIOLOGIA; JANAINA RIBEIRO COSTA ROUWS, CNPAB; LUC FELICIANUS MARIE ROUWS, CNPAB; JOSE IVO BALDANI, CNPAB; VERONICA MASSENA REIS, CNPAB. |
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Título: |
Quantifying and visualizing Nitrospirillum amazonense strain CBAmC in sugarcane after using different inoculation methods, |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plant and Soil , Published: 22 February 2023. |
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ISSN: |
1573-5036 |
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Idioma: |
Inglês |
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Conteúdo: |
Nitrospirillum amazonense strain CBAmC is a nitrogen-fixing bacterium that has been used for sugarcane inoculation. Despite its beneficial effects, however, little is known about the establishment and colonization of this microorganism in its inoculated host plant. Furthermore, alternative inoculation methods need to be tested in the sugarcane crop. The objective of the present work was to quantify and visualize CBAmC in sugarcane cultivar RB867515 after using three inoculation methods. Methods: Utilizing one node as propagation material, three bacterial inoculation methods were tested under greenhouse conditions. For up to 60 days after inoculation (DAI), qPCR was used to quantify the bacterial loads in the plant or substrate while FISH-CLSM was used to confirm the establishment and colonization profile of CBAmC in different plant tissues. Results: FISH-CLSM indicated that the CBAmC strain could establish itself on different tissues of sugarcane, especially roots. qPCR revealed variable bacterial loads in the plants inoculated according to the three methods tested. In addition, little or no bacterial loads were detected in the substrate a few days after inoculation, regardless of the method used. Conclusions: N. amazonense CBAmC proves to be a bacterium that preferentially associates with the surface of the root tissues of sugarcane propagated by one node, instead of colonizing internal plant tissues or remaining in the soil. The inoculation method consisting of immersion of the mini-setts in bacterial suspension proved to be more efficient than the inoculation methods of applying the suspension onto the post-sprouting substrate or to the lowest lea MenosNitrospirillum amazonense strain CBAmC is a nitrogen-fixing bacterium that has been used for sugarcane inoculation. Despite its beneficial effects, however, little is known about the establishment and colonization of this microorganism in its inoculated host plant. Furthermore, alternative inoculation methods need to be tested in the sugarcane crop. The objective of the present work was to quantify and visualize CBAmC in sugarcane cultivar RB867515 after using three inoculation methods. Methods: Utilizing one node as propagation material, three bacterial inoculation methods were tested under greenhouse conditions. For up to 60 days after inoculation (DAI), qPCR was used to quantify the bacterial loads in the plant or substrate while FISH-CLSM was used to confirm the establishment and colonization profile of CBAmC in different plant tissues. Results: FISH-CLSM indicated that the CBAmC strain could establish itself on different tissues of sugarcane, especially roots. qPCR revealed variable bacterial loads in the plants inoculated according to the three methods tested. In addition, little or no bacterial loads were detected in the substrate a few days after inoculation, regardless of the method used. Conclusions: N. amazonense CBAmC proves to be a bacterium that preferentially associates with the surface of the root tissues of sugarcane propagated by one node, instead of colonizing internal plant tissues or remaining in the soil. The inoculation method consisting of immersion o... Mostrar Tudo |
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Palavras-Chave: |
Nitrospirillum amazonense; Promoting plant growth. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02478naa a2200265 a 4500
001 2152151
005 2023-03-07
008 2023 bl uuuu u00u1 u #d
022 $a1573-5036
100 1 $aSCHWAB, S.
245 $aQuantifying and visualizing Nitrospirillum amazonense strain CBAmC in sugarcane after using different inoculation methods,$h[electronic resource]
260 $c2023
520 $aNitrospirillum amazonense strain CBAmC is a nitrogen-fixing bacterium that has been used for sugarcane inoculation. Despite its beneficial effects, however, little is known about the establishment and colonization of this microorganism in its inoculated host plant. Furthermore, alternative inoculation methods need to be tested in the sugarcane crop. The objective of the present work was to quantify and visualize CBAmC in sugarcane cultivar RB867515 after using three inoculation methods. Methods: Utilizing one node as propagation material, three bacterial inoculation methods were tested under greenhouse conditions. For up to 60 days after inoculation (DAI), qPCR was used to quantify the bacterial loads in the plant or substrate while FISH-CLSM was used to confirm the establishment and colonization profile of CBAmC in different plant tissues. Results: FISH-CLSM indicated that the CBAmC strain could establish itself on different tissues of sugarcane, especially roots. qPCR revealed variable bacterial loads in the plants inoculated according to the three methods tested. In addition, little or no bacterial loads were detected in the substrate a few days after inoculation, regardless of the method used. Conclusions: N. amazonense CBAmC proves to be a bacterium that preferentially associates with the surface of the root tissues of sugarcane propagated by one node, instead of colonizing internal plant tissues or remaining in the soil. The inoculation method consisting of immersion of the mini-setts in bacterial suspension proved to be more efficient than the inoculation methods of applying the suspension onto the post-sprouting substrate or to the lowest lea
653 $aNitrospirillum amazonense
653 $aPromoting plant growth
700 1 $aHIRATA, E. S.
700 1 $aAMARAL, J. C. A.
700 1 $aSILVA, C. G. N.
700 1 $aFERREIRA, J. P.
700 1 $aSILVA, L. V. da
700 1 $aROUWS, J. R. C.
700 1 $aROUWS, L. F. M.
700 1 $aBALDANI, J. I.
700 1 $aREIS, V. M.
773 $tPlant and Soil , Published: 22 February 2023.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 126 | |
| 121. |  | ROSA, J. S. da; FREITAS-SILVA, O.; ROUWS, J. R. C.; MOREIRA, I. G. da S.; NOVAES, F. J. M.; AZEVEDO, D. de A.; SCHWAB, N.; GODOY, R. L. de O.; EBERLIN, M. N.; REZENDE, C. M. de. Mass spectrometry screening of Arabica coffee roasting: A non-target and non-volatile approach by EASI-MS and ESI-MS Food Research International [online], v. 89, part 2, p. 967-975, nov. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 122. | | DALLA CHIEZA, E.; ARAUJO, E. da S.; ESPINDOLA, J. A. A.; LEAL, M. A. de A.; ROUWS, J. R. C.; URQUIAGA, S.; BODDEY, R. M.; ALVES, B. J. R. Marcação de leguminosas com 15N para estudos da dinâmica de N, derivado de adubo verde, no sistema solo-planta. Seropédica: Embrapa Agrobiologia 2011. (Embrapa Agrobiologia. Boletim de Pesquisa e Desenvolvimento, 72).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 123. | | SCHWAB, S.; HIRATA, E. S.; AMARAL, J. C. A.; SILVA, C. G. N.; FERREIRA, J. P.; SILVA, L. V. da; ROUWS, J. R. C.; ROUWS, L. F. M.; BALDANI, J. I.; REIS, V. M. Quantifying and visualizing Nitrospirillum amazonense strain CBAmC in sugarcane after using different inoculation methods, Plant and Soil , Published: 22 February 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 124. | | BENTO, V. M. V.; DIAS, A.; ROUWS, J. R. C.; LEAL, J. F.; RAMALHO, I. O.; PAULA, T. C.; XAVIER, G. R.; RUMJANEK, N. G.; RIBEIRO, R. de L. D. Production of organic chicory (Cichorium endivia) trasnplants on nutritionally enriched commercial substrate and inoculation with fluorescent Pseudomonas sp. strain SD1 In: LATTIN AMERICAN PGPR WORKSHOP, 2.; TALLER LATINOAMERICANO SOBRE RIZOBACTERIAS PROMOTORAS DEL DESARROLLO VEGETAL, 2014, Córdoba, AR. Libro de resúmes. Buenos Aires: Universidad Nacional de Quilmes, 2014. p. 87| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 125. | | MATOS, G. F. de; SANTOS, S. G. dos; BAHIA, P. P.; COELHO, M. R. R.; BALDANI, J. I.; ROUWS, J. R. C.; REIS, V. M.; URQUIAGA, S.; ROUWS, L. F. M. Positive effects of endophytic (Brady) rhizobium isolates on sugarcane sett germination and root growth under glass house conditions. In: INTERNATIONAL PGPR WORKSHOP, 10., liége, 2015. Program and abstract book. Liége: Université de Liége, 2015 p. 86| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 126. | | SOARES, J. P. G.; DIAS, J.; ALMEIDA, D. L. de; GUERRA, J. G. M.; SILVA, S. N. da; OLIVEIRA, A. D. de; SALMAN, A. K. D.; ROUWS, J. R. C. Produção orgânica de capim elefante em consórcio com siratro sob manejo de cortes. In: CONGRESSO BRASILEIRO DE AGROECOLOGIA, 4., 2006, Belo Horizonte. Construindo horizontes sustentáveis: anais. Belo Horizonte: Emater-MG, 2006. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Rondônia. |
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| Registros recuperados : 126 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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