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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Soja. |
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Data corrente: |
06/01/1993 |
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Data da última atualização: |
06/01/1993 |
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Autoria: |
GRIFFITH, G. W.; ROUGHLEY, R. J. |
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Afiliação: |
Horticultural Research and Advisory Station, PO Box 581, Gosford, NSW 2250, Australia. |
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Título: |
The effect of moisture potential on growth and survival of root nodule bacteria in peat culture and on seed. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Journal of Applied Bacteriology, v. 73, p.7-13, 1992. |
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Idioma: |
Inglês
Português |
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Conteúdo: |
Peat from three sources was dried, milled and packed separately in polyethylene bags and sterilized by irradiation. The carrier was impregnat with broth cultures of either Rhizobium leguminosarum bv. trifolii strain WU95, Bradyrhizobium japonicum strain CB1809 or B. lupini strin WU425 and sterile water to provide five moisture potentials in the range > -1 x 10 4- - 1 x 10 6 Pa. The packets were stored at 26.C under conditions which restricted moisture loss. Numbers of root nodule bacteria were conted at intervals up to 12 weeks. No single moisture potnetial was optimum for all strains in all carriers because of a significant (P<0.05) interaction between moisture potential x strain x carrierx time. Where direct comparisons could be made, all strains survived best at 1 x 10 4 and /or - 3.2 x 10 4 Pa. Seeds of Trifolium subterraneum and polypropylene beads (used to avoid seed coat toxicity) were inoculated with WU95 prepared in two sources of peat and at each of the above moisture potentials and store at 15.C. Seed coat toxicity significantly effected the log death rate (K) of WU95 on subterraneum clover seed for the period 0-0.25 d (K1.796) compared with K -0.399 for polypropylene beads. In the first 24h moisture did not affect survival but by 28 rhizobia grown in badenock peat survivel best at - 3.2 x 10 4 and 1-10 4. Pa. |
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Categoria do assunto: |
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Marc: |
LEADER 01766naa a2200133 a 4500
001 1457690
005 1993-01-06
008 1992 bl --- 0-- u #d
100 1 $aGRIFFITH, G. W.
245 $aThe effect of moisture potential on growth and survival of root nodule bacteria in peat culture and on seed.
260 $c1992
520 $aPeat from three sources was dried, milled and packed separately in polyethylene bags and sterilized by irradiation. The carrier was impregnat with broth cultures of either Rhizobium leguminosarum bv. trifolii strain WU95, Bradyrhizobium japonicum strain CB1809 or B. lupini strin WU425 and sterile water to provide five moisture potentials in the range > -1 x 10 4- - 1 x 10 6 Pa. The packets were stored at 26.C under conditions which restricted moisture loss. Numbers of root nodule bacteria were conted at intervals up to 12 weeks. No single moisture potnetial was optimum for all strains in all carriers because of a significant (P<0.05) interaction between moisture potential x strain x carrierx time. Where direct comparisons could be made, all strains survived best at 1 x 10 4 and /or - 3.2 x 10 4 Pa. Seeds of Trifolium subterraneum and polypropylene beads (used to avoid seed coat toxicity) were inoculated with WU95 prepared in two sources of peat and at each of the above moisture potentials and store at 15.C. Seed coat toxicity significantly effected the log death rate (K) of WU95 on subterraneum clover seed for the period 0-0.25 d (K1.796) compared with K -0.399 for polypropylene beads. In the first 24h moisture did not affect survival but by 28 rhizobia grown in badenock peat survivel best at - 3.2 x 10 4 and 1-10 4. Pa.
700 1 $aROUGHLEY, R. J.
773 $tJournal of Applied Bacteriology$gv. 73, p.7-13, 1992.
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Registro original: |
Embrapa Soja (CNPSO) |
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