Data/hora: 24/09/2024 04:43:27

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	20/05/2014	
Data da última atualização: 	05/02/2024	
Autoria: 	ROSSE, I. da C.; STEINBERG, R. da S.; COIMBRA, R. S.; PEIXOTO, M. G. C. D.; VERNEQUE, R. da S.; MACHADO, M. A.; FONSECA, C. G.; CARVALHO, M. R. S.	
Afiliação: 	IZINARA DA CRUZ ROSSE, UFMG; RAPHAEL DA SILVA STEINBERG, UFMG; RONEY SANTOS COIMBRA, FIOCRUZ-Minas; MARIA GABRIELA CAMPOLINA D PEIXOTO, CNPGL; RUI DA SILVA VERNEQUE, CNPGL; MARCO ANTONIO MACHADO, CNPGL; CLEUSA GRAÇA FONSECA, UFMG; MARIA RAQUEL SANTOS CARVALHO, UFMG.	
Título: 	Novel SNPs and INDEL polymorphisms in the 30UTR of DGAT1 gene: in silico analyses and a possible association.	
Ano de publicação: 	2014	
Fonte/Imprenta: 	Molecular Biological Reproduction, v. 41, n. 7, p. 4555-4563, 2014.	
DOI: 	https://doi.org/10.1007/s11033-014-3326-z	
Idioma: 	Inglês	
Conteúdo: 	Diacylglycerol-O-acyltransferase (DGAT1) gene encodes the rate-limiting enzyme of triglyceride synthesis. A polymorphism in this gene, DGAT1 K232A, has been associated with milk production and composition in taurine breeds. However, this polymorphism is not a good tool for ascertaining the effects of this QTL in Bos indicus (Zebu), since the frequency of the DGAT1 232A allele is too low in these breeds. We sequenced the 3'-untranslated region of DGAT1 gene in a sample of bulls of the breeds Guzerá (Bos indicus) and Holstein (Bos taurus) and, using in silico analysis, we searched for genetic variation, evolutionary conservation, regulatory elements, and possible substitution effects. Six single nucleotide (SNPs) and one insertion-deletion (INDEL) polymorphisms were found in the Guzerá bulls. Additionally, we developed a preliminary association study, using this INDEL polymorphism as a genetic marker. A significant association was detected (P ≤ 0.05) between the INDEL (DGAT1 3'UTR INDEL) and the breeding values (BV) for protein, fat, and milk yields over a 305-day lactation period. The DGAT1 3' UTR INDEL genotype I/I (I, for insertion) was associated with lower BVs (-38.77 kg for milk, -1.86 kg for fat, and -1.48 kg for protein yields), when compared to the genotype I/D (D, for deletion). I/D genotype was lower D/D genotype (-34.98 kg milk, -1.73 kg fat, and -1.09 kg protein yields). This study reports the first polymorphism of DGAT1 3'UTR in the Guzerá breed, as well as its association with BV for milk protein, fat, and milk yields.	
Palavras-Chave: 	3'Untranslated region; DGAT1; Genetic association study.	
Thesagro: 	Bos Indicus.	
Categoria do assunto: 	G Melhoramento Genético	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (21360-1) - UPC - AP - DD -  -  -  - DIS