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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
16/02/2012 |
Data da última atualização: |
24/02/2023 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
MATIDA, E. T.; KUNINARI-NASCIMENTO, R.; SANCHES, C. C.; ROSINHA, G. M. S.; DRIEMEIER, D.; BLOCH JUNIOR, C.; SOARES, C. O.; VERBISCK, N. V. |
Afiliação: |
ELIZANGELA TIEKO MATIDA, UFMS; UFMS; UFMS; GRACIA MARIA SOARES ROSINHA, CNPGC; Professor da Universidade Federal do Rio Grande do Sul.; CARLOS BLOCH JUNIOR, CENARGEN; CLEBER OLIVEIRA SOARES, CNPGC; NEWTON VALERIO VERBISCK, CNPGC. |
Título: |
Aplicação de espectrometria de massas MALDI-TOF para identificação de candidatos a marcadores moleculares de Scrapie. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 7., 2011, CAMPO GRANDE, MS. [Anais da]... Campo Grande, MS: Embrapa Gado de Corte, 2011. |
Idioma: |
Português |
Conteúdo: |
O trabalho aqui apresentado busca identificar marcadores moleculares para diagnóstico de Scrapie em amostras de sangue de animais acometidos por PrPSc. |
Palavras-Chave: |
Crioula/Pantaneira; Encefalopatia Espongiforme; MALDI-TOF; Ovelhas; Suffolk. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/915795/1/1DOC1861-1.pdf
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Marc: |
LEADER 01017nam a2200253 a 4500 001 1915795 005 2023-02-24 008 2011 bl uuuu u00u1 u #d 100 1 $aMATIDA, E. T. 245 $aAplicação de espectrometria de massas MALDI-TOF para identificação de candidatos a marcadores moleculares de Scrapie.$h[electronic resource] 260 $aIn: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 7., 2011, CAMPO GRANDE, MS. [Anais da]... Campo Grande, MS: Embrapa Gado de Corte$c2011 520 $aO trabalho aqui apresentado busca identificar marcadores moleculares para diagnóstico de Scrapie em amostras de sangue de animais acometidos por PrPSc. 653 $aCrioula/Pantaneira 653 $aEncefalopatia Espongiforme 653 $aMALDI-TOF 653 $aOvelhas 653 $aSuffolk 700 1 $aKUNINARI-NASCIMENTO, R. 700 1 $aSANCHES, C. C. 700 1 $aROSINHA, G. M. S. 700 1 $aDRIEMEIER, D. 700 1 $aBLOCH JUNIOR, C. 700 1 $aSOARES, C. O. 700 1 $aVERBISCK, N. V.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
Biblioteca(s): |
Embrapa Agroindústria Tropical. |
Data corrente: |
27/05/2022 |
Data da última atualização: |
27/05/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
AMORIM, C. C. de; SILVA, F. D. da; BORDALLO, P. do N.; ARAGAO, F. A. S. de; QUEIRÓZ, M. A. de. |
Afiliação: |
CLISNEIDE COELHO DE AMORIM, Programa de Pós-Graduação em Recursos Genéticos Vegetais, Universidade Estadual de Feira de Santana (UEFS); FRANCISCO DAVI DA SILVA, Universidade Federal de Viçosa (UFV); PATRICIA DO NASCIMENTO BORDALLO, CNPAT; FERNANDO ANTONIO SOUZA DE ARAGAO, CNPAT; MANOEL ABÍLIO DE QUEIRÓZ, Universidade do Estado da Bahia (UNEB), Campus Juazeiro-BA. |
Título: |
Genetic divergence between sub-accessions of the melon using molecular markers. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Revista Ciência Agronômica, v. 53, e20218007, 2022. |
DOI: |
DOI: 10.5935/1806-6690.20220027 |
Idioma: |
Inglês |
Conteúdo: |
The melon (Cucumis melo L.) is one of the most important horticultural crops in the northeast of Brazil. The characterisation of germplasm is an ef ficient process for managing plant genetic resources, as it contributes to better conservation and improved use in breeding programs. In the present study, the genetic divergence between sub-accessions of the melon taken from traditional agriculture in the semi-arid region of Brazil was evaluated using ISSR and RAPD markers, verifying the level of agreement with the botanical classification. Twenty-six sub-accessions preserved in the Active Germplasm Bank of Cucurbitaceae for the northeast of Brazil, located at Embrapa Semiárido in Petrolina, Pernambuco, were analysed. The binary matrix of the molecular data was used to obtain the genetic dissimilarities using the complement of the Jaccard index. The genetic dissimilarities were represented in dendrograms generated using the UPGMA method. Twenty-eight ISSR primers and 26 RAPD primers were polymorphic. A total of 686 fragments were obtained, of which 451 were polymorphic. The mean polymorphism of the RAPD markers was greater than that of the ISSR markers. The number of markers was considered suf ficient to accurately determine the genetic diversity. The ISSR dendrogram allowed the formation of three groups, the RAPD dendrogram, four groups, and the joint dendrogram, three groups. The study demonstrated the genetic variability between the sub-accessions, and the strong association between the characterisation made by the molecular markers and the taxonomic classification of the genotypes under study. MenosThe melon (Cucumis melo L.) is one of the most important horticultural crops in the northeast of Brazil. The characterisation of germplasm is an ef ficient process for managing plant genetic resources, as it contributes to better conservation and improved use in breeding programs. In the present study, the genetic divergence between sub-accessions of the melon taken from traditional agriculture in the semi-arid region of Brazil was evaluated using ISSR and RAPD markers, verifying the level of agreement with the botanical classification. Twenty-six sub-accessions preserved in the Active Germplasm Bank of Cucurbitaceae for the northeast of Brazil, located at Embrapa Semiárido in Petrolina, Pernambuco, were analysed. The binary matrix of the molecular data was used to obtain the genetic dissimilarities using the complement of the Jaccard index. The genetic dissimilarities were represented in dendrograms generated using the UPGMA method. Twenty-eight ISSR primers and 26 RAPD primers were polymorphic. A total of 686 fragments were obtained, of which 451 were polymorphic. The mean polymorphism of the RAPD markers was greater than that of the ISSR markers. The number of markers was considered suf ficient to accurately determine the genetic diversity. The ISSR dendrogram allowed the formation of three groups, the RAPD dendrogram, four groups, and the joint dendrogram, three groups. The study demonstrated the genetic variability between the sub-accessions, and the strong association ... Mostrar Tudo |
Palavras-Chave: |
Análise de agrupamento; Caracterização molecular; Diversidade genética; Molecular characterisation. |
Thesagro: |
Biodiversidade; Cucumis Melo; Hortaliça; Melão; Melhoramento Genético Vegetal. |
Thesaurus NAL: |
Biodiversity; Cluster analysis; Melons. |
Categoria do assunto: |
-- G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1143502/1/ART22047.pdf
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Marc: |
LEADER 02600naa a2200325 a 4500 001 2143502 005 2022-05-27 008 2022 bl uuuu u00u1 u #d 024 7 $aDOI: 10.5935/1806-6690.20220027$2DOI 100 1 $aAMORIM, C. C. de 245 $aGenetic divergence between sub-accessions of the melon using molecular markers.$h[electronic resource] 260 $c2022 520 $aThe melon (Cucumis melo L.) is one of the most important horticultural crops in the northeast of Brazil. The characterisation of germplasm is an ef ficient process for managing plant genetic resources, as it contributes to better conservation and improved use in breeding programs. In the present study, the genetic divergence between sub-accessions of the melon taken from traditional agriculture in the semi-arid region of Brazil was evaluated using ISSR and RAPD markers, verifying the level of agreement with the botanical classification. Twenty-six sub-accessions preserved in the Active Germplasm Bank of Cucurbitaceae for the northeast of Brazil, located at Embrapa Semiárido in Petrolina, Pernambuco, were analysed. The binary matrix of the molecular data was used to obtain the genetic dissimilarities using the complement of the Jaccard index. The genetic dissimilarities were represented in dendrograms generated using the UPGMA method. Twenty-eight ISSR primers and 26 RAPD primers were polymorphic. A total of 686 fragments were obtained, of which 451 were polymorphic. The mean polymorphism of the RAPD markers was greater than that of the ISSR markers. The number of markers was considered suf ficient to accurately determine the genetic diversity. The ISSR dendrogram allowed the formation of three groups, the RAPD dendrogram, four groups, and the joint dendrogram, three groups. The study demonstrated the genetic variability between the sub-accessions, and the strong association between the characterisation made by the molecular markers and the taxonomic classification of the genotypes under study. 650 $aBiodiversity 650 $aCluster analysis 650 $aMelons 650 $aBiodiversidade 650 $aCucumis Melo 650 $aHortaliça 650 $aMelão 650 $aMelhoramento Genético Vegetal 653 $aAnálise de agrupamento 653 $aCaracterização molecular 653 $aDiversidade genética 653 $aMolecular characterisation 700 1 $aSILVA, F. D. da 700 1 $aBORDALLO, P. do N. 700 1 $aARAGAO, F. A. S. de 700 1 $aQUEIRÓZ, M. A. de 773 $tRevista Ciência Agronômica$gv. 53, e20218007, 2022.
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