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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
05/01/2016 |
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Data da última atualização: |
17/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEGORARO, C.; TADIELLO, A.; GIRARDI, C. L.; CHAVES, F. C.; QUECINI, V.; OLIVEIRA, A. C. de; TRAINOTTI, L.; ROMBALDI, C. V. |
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Afiliação: |
CESAR LUIS GIRARDI, CNPUV; VERA MARIA QUECINI, CNPUV. |
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Título: |
Transcriptional regulatory networks controlling woolliness in peach in response to preharvest gibberellin application and cold storage |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
BMC Plant Biology, v. 15, n. 1, p. 279, 2015. |
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Idioma: |
Português |
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Conteúdo: |
Abstract Background: Postharvest fruit conservation relies on low temperatures and manipulations of hormone metabolism to maintain sensory properties. Peaches are susceptible to chilling injuries, such as ?woolliness? that is caused by juice loss leading to a ?wooly? fruit texture. Application of gibberellic acid at the initial stages of pit hardening impairs woolliness incidence, however the mechanisms controlling the response remain unknown. We have employed genome wide transcriptional profiling to investigate the effects of gibberellic acid application and cold storage on harvested peaches. Results: Approximately half of the investigated genes exhibited significant differential expression in response to the treatments. Cellular and developmental process gene ontologies were overrepresented among the differentially regulated genes, whereas sequences in cell death and immune response categories were underrepresented. Gene set enrichment demonstrated a predominant role of cold storage in repressing the transcription of genes associated to cell wall metabolism. In contrast, genes involved in hormone responses exhibited a more complex transcriptional response, indicating an extensive network of crosstalk between hormone signaling and low temperatures. Time course transcriptional analyses demonstrate the large contribution of gene expression regulation on the biochemical changes leading to woolliness in peach. Conclusion: Overall, our results provide insights on the mechanisms controlling the complex phenotypes associated to postharvest textural changes in peach and suggest that hormone mediated reprogramming previous to pit hardening affects the onset of chilling injuries. Keywords: Chilling injury, Cold storage, Genome wide gene expression, Gibberellic acid, Prunus persica MenosAbstract Background: Postharvest fruit conservation relies on low temperatures and manipulations of hormone metabolism to maintain sensory properties. Peaches are susceptible to chilling injuries, such as ?woolliness? that is caused by juice loss leading to a ?wooly? fruit texture. Application of gibberellic acid at the initial stages of pit hardening impairs woolliness incidence, however the mechanisms controlling the response remain unknown. We have employed genome wide transcriptional profiling to investigate the effects of gibberellic acid application and cold storage on harvested peaches. Results: Approximately half of the investigated genes exhibited significant differential expression in response to the treatments. Cellular and developmental process gene ontologies were overrepresented among the differentially regulated genes, whereas sequences in cell death and immune response categories were underrepresented. Gene set enrichment demonstrated a predominant role of cold storage in repressing the transcription of genes associated to cell wall metabolism. In contrast, genes involved in hormone responses exhibited a more complex transcriptional response, indicating an extensive network of crosstalk between hormone signaling and low temperatures. Time course transcriptional analyses demonstrate the large contribution of gene expression regulation on the biochemical changes leading to woolliness in peach. Conclusion: Overall, our results provide insights on the mechanisms ... Mostrar Tudo |
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Thesagro: |
Prunus Persica. |
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Thesaurus Nal: |
Chilling injury; cold storage; Gene expression; Genome; Gibberellic acid. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136584/1/PEGORARO-BMCPLANTBIOLOGY-V15-P279-2015.pdf
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Marc: |
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100 1 $aPEGORARO, C.
245 $aTranscriptional regulatory networks controlling woolliness in peach in response to preharvest gibberellin application and cold storage$h[electronic resource]
260 $c2015
520 $aAbstract Background: Postharvest fruit conservation relies on low temperatures and manipulations of hormone metabolism to maintain sensory properties. Peaches are susceptible to chilling injuries, such as ?woolliness? that is caused by juice loss leading to a ?wooly? fruit texture. Application of gibberellic acid at the initial stages of pit hardening impairs woolliness incidence, however the mechanisms controlling the response remain unknown. We have employed genome wide transcriptional profiling to investigate the effects of gibberellic acid application and cold storage on harvested peaches. Results: Approximately half of the investigated genes exhibited significant differential expression in response to the treatments. Cellular and developmental process gene ontologies were overrepresented among the differentially regulated genes, whereas sequences in cell death and immune response categories were underrepresented. Gene set enrichment demonstrated a predominant role of cold storage in repressing the transcription of genes associated to cell wall metabolism. In contrast, genes involved in hormone responses exhibited a more complex transcriptional response, indicating an extensive network of crosstalk between hormone signaling and low temperatures. Time course transcriptional analyses demonstrate the large contribution of gene expression regulation on the biochemical changes leading to woolliness in peach. Conclusion: Overall, our results provide insights on the mechanisms controlling the complex phenotypes associated to postharvest textural changes in peach and suggest that hormone mediated reprogramming previous to pit hardening affects the onset of chilling injuries. Keywords: Chilling injury, Cold storage, Genome wide gene expression, Gibberellic acid, Prunus persica
650 $aChilling injury
650 $acold storage
650 $aGene expression
650 $aGenome
650 $aGibberellic acid
650 $aPrunus Persica
700 1 $aTADIELLO, A.
700 1 $aGIRARDI, C. L.
700 1 $aCHAVES, F. C.
700 1 $aQUECINI, V.
700 1 $aOLIVEIRA, A. C. de
700 1 $aTRAINOTTI, L.
700 1 $aROMBALDI, C. V.
773 $tBMC Plant Biology$gv. 15, n. 1, p. 279, 2015.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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