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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
26/08/2014 |
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Data da última atualização: |
19/06/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOMES, D. F.; BATISTA, J. S. da S.; ROLLA, A. A. P.; SILVA, L. P. da; BLOCH, C.; GALLI-TARASAWA, L. V.; HUNGRIA, M. |
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Afiliação: |
DOUGLAS FABIANO GOMES, Departamento de Génetica UFPR; JESSIANE STEFÂNIA DA SILVA BATISTA, DEBIOGEM UEPG; AMANDA ALVES PAIVA ROLLA; LUCIANO PAULINO DA SILVA, CENARGEN; CARLOS BLOCH JUNIOR, Cenargen; LYGIA VITORIA GALLI TERASAWA, Departamento de Génetica UFPR; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Genomics, v. 15, n. 643, 2014. |
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DOI: |
10.1186/1471-2164-15-643 |
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Idioma: |
Português |
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Conteúdo: |
Strain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol monophosphatase, IMPase), fixation of atmospheric nitrogen (N 2) (e.g. NifH) and defenses against stresses (e.g. chaperones). By using bioinformatic tools, it was possible to attribute probable functions to ten hypothetical proteins. For another ten proteins classified as? NO related COG? group, we analyzed by RT-qPCR the relative expression of their coding-genes in response to the nodulation-gene inducer genistein. Six of these genes were up-regulated, including blr0227, which may be related to polyhydroxybutyrate (PHB) biosynthesis and competitiveness for nodulation. The proteomic map contributed to the identification of several proteins of B. diazoefficiens under free-living conditions and our approach? combining bioinformatics and gene-expression assays ? resulted in new information about unknown genes that might play important roles in the establishment of the symbiosis with soybean. MenosStrain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol monophosphatase, IMPase), fixation of atmospheric nitrogen (N 2) (e.g. NifH) and defenses against stresses (e.g. chaperones). By using bioinformatic tools, it was possible to attribute probable functions to ten hypothetical proteins. For another ten proteins classified as? NO related COG? group, we analyzed by RT-qPCR the relative expression of their coding-genes in response to the nodulation-gene inducer genistein. Six of these genes were up-regulated, including blr0227, which may be related to polyhydroxybutyrate (PHB) biosynthesis and competitiveness for nodulation. The proteomic map contributed to the identification of several proteins of B. diazoefficiens under free-living conditions and our approach? combining bioinformatics... Mostrar Tudo |
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Palavras-Chave: |
RT-qPCR; Two-dimensional proteomics. |
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Thesaurus Nal: |
Bradyrhizobium; nitrogen fixation; symbiosis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160837/1/12864-2014-Article-6775.pdf
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Marc: |
LEADER 02463naa a2200265 a 4500
001 1993458
005 2017-06-19
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1186/1471-2164-15-643$2DOI
100 1 $aGOMES, D. F.
245 $aProteomic analysis of free-living Bradyrhizobium diazoefficiens$bhighlighting potential determinants of a successful symbiosis$h[electronic resource]
260 $c2014
520 $aStrain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol monophosphatase, IMPase), fixation of atmospheric nitrogen (N 2) (e.g. NifH) and defenses against stresses (e.g. chaperones). By using bioinformatic tools, it was possible to attribute probable functions to ten hypothetical proteins. For another ten proteins classified as? NO related COG? group, we analyzed by RT-qPCR the relative expression of their coding-genes in response to the nodulation-gene inducer genistein. Six of these genes were up-regulated, including blr0227, which may be related to polyhydroxybutyrate (PHB) biosynthesis and competitiveness for nodulation. The proteomic map contributed to the identification of several proteins of B. diazoefficiens under free-living conditions and our approach? combining bioinformatics and gene-expression assays ? resulted in new information about unknown genes that might play important roles in the establishment of the symbiosis with soybean.
650 $aBradyrhizobium
650 $anitrogen fixation
650 $asymbiosis
653 $aRT-qPCR
653 $aTwo-dimensional proteomics
700 1 $aBATISTA, J. S. da S.
700 1 $aROLLA, A. A. P.
700 1 $aSILVA, L. P. da
700 1 $aBLOCH, C.
700 1 $aGALLI-TARASAWA, L. V.
700 1 $aHUNGRIA, M.
773 $tBMC Genomics$gv. 15, n. 643, 2014.
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Registro original: |
Embrapa Soja (CNPSO) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
11/05/2022 |
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Data da última atualização: |
12/05/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
FILGUEIRAS, M. D. G.; MATOS, R.; BARRETO, L. P.; RIZZO, P. V.; FREITAS, F. M. C.; MASCARIN, G. M.; PRATA, M. C. de A.; MONTEIRO, C.; FERNANDES, E. K. K. |
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Afiliação: |
MARCOS DANIEL G. FILGUEIRAS, Universidade Federal de Goiás; RENATA MATOS; LUCAS PRADO BARRETO, Universidade Federal de Goiás; PRICILA VETRANO RIZZO, CNPGL; FERNANDA MARA CUNHA FREITAS, CNPGL; GABRIEL MOURA MASCARIN, CNPMA; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; CAIO MONTEIRO, Universidade Federal de Goiás; ÉVERTON KORT KAMP FERNANDES, Universidade Federal de Goiás. |
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Título: |
Potencial de hetororhabditis bacteriophora HP88 (Nematoda: heterorhabditidae) para controle de Rhipicephalus microplus (Acari: ixodidae): do laboratório para o campo. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE ACAROLOGIA, 7., 2021. Acarologia em tempos de conectividade: [resumos]. México: Sociedad Latinoamericana de Acarologia, 2022. |
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Idioma: |
Português |
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Notas: |
Evento online. |
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Conteúdo: |
O presente estudo avaliou, em laboratório, a patogenicidade do nematoide Heterorhabditis bacteriophora HP88 sobre fêmeas de Rhipicephalus microplus com diferentes níveis de ingurgitamento (150, 200, 250, 300 e 350 mg), e sobre fêmeas provenientes de diferentes populações (1 a 8). |
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Palavras-Chave: |
Nematoide entomopatogênico. |
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Thesagro: |
Bovino; Carrapato; Controle Biológico; Nematóide. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01278nam a2200277 a 4500
001 2142882
005 2022-05-12
008 2022 bl uuuu u01u1 u #d
100 1 $aFILGUEIRAS, M. D. G.
245 $aPotencial de hetororhabditis bacteriophora HP88 (Nematoda$bheterorhabditidae) para controle de Rhipicephalus microplus (Acari: ixodidae): do laboratório para o campo.$h[electronic resource]
260 $aIn: SIMPÓSIO BRASILEIRO DE ACAROLOGIA, 7., 2021. Acarologia em tempos de conectividade: [resumos]. México: Sociedad Latinoamericana de Acarologia$c2022
500 $aEvento online.
520 $aO presente estudo avaliou, em laboratório, a patogenicidade do nematoide Heterorhabditis bacteriophora HP88 sobre fêmeas de Rhipicephalus microplus com diferentes níveis de ingurgitamento (150, 200, 250, 300 e 350 mg), e sobre fêmeas provenientes de diferentes populações (1 a 8).
650 $aBovino
650 $aCarrapato
650 $aControle Biológico
650 $aNematóide
653 $aNematoide entomopatogênico
700 1 $aMATOS, R.
700 1 $aBARRETO, L. P.
700 1 $aRIZZO, P. V.
700 1 $aFREITAS, F. M. C.
700 1 $aMASCARIN, G. M.
700 1 $aPRATA, M. C. de A.
700 1 $aMONTEIRO, C.
700 1 $aFERNANDES, E. K. K.
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Embrapa Meio Ambiente (CNPMA) |
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