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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
27/03/2002 |
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Data da última atualização: |
27/03/2002 |
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Autoria: |
KELEMU, S.; SKINNER, D.; BADEL; J. L.; MORENO, C. X.; RODRIGUEZ, M. X.; FERNANDES, C. D.; CHARCHAR, M. J.; CHAKRABORTY, S. |
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Título: |
Genetic diversity in South American Colletottrichum goleosporioides isolates from Stylosanthes guianensis, a tropical forage legume. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
European Journal of Plant Pathology, v. 105, p. 261-272, 1999. |
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Idioma: |
Inglês |
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Conteúdo: |
The degree of genetic diversity of 127 Colletotrichum gloeosporiodes isolates from Stylosanthes guianensis genotypes in South America was measured at the molecular level by random amplified polymorphic DNA (RAPD) with mine arbitrary primers of 10 bases, and by restriction fragment length polymorphism (RFLP) with a non LTR (long terminal repeats) retrotransposon DNA sequence. The RAPD products revealed scorable polymorphism among the isolates , and a total of 80 band positions were scored. Sixty three of the 127 isolates were clustered into 13 distinct lineages usually correlating with geographic origin. Where isolates from various regions were clustered together, most had identical host genotype origin. The pathogen population sampled from Carimagua. Colombia, a long time Stylosanthes breeding and selection site, with a savanna ecosystemj, was highly diverse. A set of 12 S. guianensis genotype differentials was used to characterize pathogenic variability of 104 isolates and their virulence patterns were grouped into 57 pathotypes. However, when they were tested on four Australian differentials, they grouped into 11 pathotypes. As shown in previous studies, no strict correlations existed between genetic diversity measured by RAPD or RFLP and pathotype defined by pathogenicity pattern on the differentials. Southern blot analysis of the 127 isolates revealed 23 hybridizing fragments resulting in 41 fingerprint patterns among the 127 isolates. Relationships between RAPD or RFLP variables were examined using Spearmans Rank Correlation Coefficienyt, which showed that the two measures of genotypic variation are in agreement. MenosThe degree of genetic diversity of 127 Colletotrichum gloeosporiodes isolates from Stylosanthes guianensis genotypes in South America was measured at the molecular level by random amplified polymorphic DNA (RAPD) with mine arbitrary primers of 10 bases, and by restriction fragment length polymorphism (RFLP) with a non LTR (long terminal repeats) retrotransposon DNA sequence. The RAPD products revealed scorable polymorphism among the isolates , and a total of 80 band positions were scored. Sixty three of the 127 isolates were clustered into 13 distinct lineages usually correlating with geographic origin. Where isolates from various regions were clustered together, most had identical host genotype origin. The pathogen population sampled from Carimagua. Colombia, a long time Stylosanthes breeding and selection site, with a savanna ecosystemj, was highly diverse. A set of 12 S. guianensis genotype differentials was used to characterize pathogenic variability of 104 isolates and their virulence patterns were grouped into 57 pathotypes. However, when they were tested on four Australian differentials, they grouped into 11 pathotypes. As shown in previous studies, no strict correlations existed between genetic diversity measured by RAPD or RFLP and pathotype defined by pathogenicity pattern on the differentials. Southern blot analysis of the 127 isolates revealed 23 hybridizing fragments resulting in 41 fingerprint patterns among the 127 isolates. Relationships between RAPD or RFLP ... Mostrar Tudo |
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Palavras-Chave: |
Anthracnosis; Colletottrichum goleosporioides; Plant diseases. |
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Thesagro: |
Antracnose; Doença de Planta; Stylosanthes Guianensis. |
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Categoria do assunto: |
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Marc: |
LEADER 02501naa a2200289 a 4500
001 1564563
005 2002-03-27
008 1999 bl --- 0-- u #d
100 1 $aKELEMU, S.
245 $aGenetic diversity in South American Colletottrichum goleosporioides isolates from Stylosanthes guianensis, a tropical forage legume.
260 $c1999
520 $aThe degree of genetic diversity of 127 Colletotrichum gloeosporiodes isolates from Stylosanthes guianensis genotypes in South America was measured at the molecular level by random amplified polymorphic DNA (RAPD) with mine arbitrary primers of 10 bases, and by restriction fragment length polymorphism (RFLP) with a non LTR (long terminal repeats) retrotransposon DNA sequence. The RAPD products revealed scorable polymorphism among the isolates , and a total of 80 band positions were scored. Sixty three of the 127 isolates were clustered into 13 distinct lineages usually correlating with geographic origin. Where isolates from various regions were clustered together, most had identical host genotype origin. The pathogen population sampled from Carimagua. Colombia, a long time Stylosanthes breeding and selection site, with a savanna ecosystemj, was highly diverse. A set of 12 S. guianensis genotype differentials was used to characterize pathogenic variability of 104 isolates and their virulence patterns were grouped into 57 pathotypes. However, when they were tested on four Australian differentials, they grouped into 11 pathotypes. As shown in previous studies, no strict correlations existed between genetic diversity measured by RAPD or RFLP and pathotype defined by pathogenicity pattern on the differentials. Southern blot analysis of the 127 isolates revealed 23 hybridizing fragments resulting in 41 fingerprint patterns among the 127 isolates. Relationships between RAPD or RFLP variables were examined using Spearmans Rank Correlation Coefficienyt, which showed that the two measures of genotypic variation are in agreement.
650 $aAntracnose
650 $aDoença de Planta
650 $aStylosanthes Guianensis
653 $aAnthracnosis
653 $aColletottrichum goleosporioides
653 $aPlant diseases
700 1 $aSKINNER, D.
700 1 $aBADEL
700 1 $aJ. L.
700 1 $aMORENO, C. X.
700 1 $aRODRIGUEZ, M. X.
700 1 $aFERNANDES, C. D.
700 1 $aCHARCHAR, M. J.
700 1 $aCHAKRABORTY, S.
773 $tEuropean Journal of Plant Pathology$gv. 105, p. 261-272, 1999.
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Registro original: |
Embrapa Cerrados (CPAC) |
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