Data/hora: 25/06/2024 00:55:53

Biblioteca(s): 	Embrapa Caprinos e Ovinos.	
Data corrente: 	10/11/2005	
Data da última atualização: 	10/11/2005	
Autoria: 	MARTINEZ RODRÍGUEZ, H. A.; RAMIREZ ÁLVAREZ, H.; TÓRTORA PÉREZ, J.; AGUILAR SETIÉN, G. I.; MONTARAZ CRESPO, J. A.	
Título: 	Efecto del virus de artritis encefalitis caprina en el aparato reproductor de machos caprinos.	
Ano de publicação: 	2005	
Fonte/Imprenta: 	Veterinaria México, v. 36, n. 2, p. 159-176, abr./jun. 2005.	
Idioma: 	Espanhol	
Conteúdo: 	The effect of the Caprine Arthritis Encephalitis Virus (CAEV) on the reproductive system of the male goat was evaluated. Fourteen adult males distributed into 4 groups were studied: I) negative contrai (uninfected animais) (n;3); 11) animais experimentally infected with an autochthonous CAEV (FES-C.UNAM Isolate) (n;3); 111) naturally infected animais (n;5); IV) animais experimentally infected with CAEV reference strain from the American Type Culture Collection (ATCC) (n;3). Blood and semen samples were collected every 30 days and ani- mais were clinically evaluated (especially for possible external genital alterations) during 10 months. No differences in scrotal diameter, seminal motility, seminal pH, colar and volume were found among the four groups. Antibodies against CAEV were detected by ELISA in blood serum and seminal ftuid in animais infected with the autochthonous strain after 7 months, in animais experimentally infected with the reference strain after 3 months and animais naturally infected remained positive throughout the experimento By Western Blot, antibodies against proteins p14, p16, p19, p25, p27, p45, p71, p90 and gp135 thousand kDa were found in seminal ftuid and in blood serum against proteins p16, p1g, p25, p27, p71, p90, and gp135 thousand kDa. Animais were humanely sacrificed 10 months after inoculation. No pathological altera- .tions were found in the genital organs of animais belonging to groups I and IV. In group 11 only one animal was found with a granuloma in the head .of the epididymis of the right testis with a yellowish creamy substance. In group III one animal was found with hydrocele that contained a serous liquid, another with edema in the head of the right testis and another one with syncytia in seminal vesicle. Immunohistochemical studies showed CAEV antigens in epithelial cells of the seminal vesicles, ampulla and bulbourethral glands in groups 11, 111 and IV. By PCR, CAEV was detected in seminal ftuid at the time of sacrifice. No histological altera- tions were found except in one animal belonging to group 111 which presented syncytia in the seminal vesicle. The presence of antigen in the tissues demonstrates that these may act as disseminators of CAE. 	
Palavras-Chave: 	Artrite encefalite caprina; CAEV; Doença aninal; Sistema reprodutivo.	
Thesagro: 	Caprino; Sêmen.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Caprinos e Ovinos (CNPC)	
Exemplares: 	CNPC - (18090-1) - ADC - AP - -- -  -  - v.36,p.159 - DIS