Data/hora: 14/06/2024 16:16:52

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 07/05/2013
Data da última atualização: | 17/01/2024
Autoria: | RODRIGUES, G. Q.; SILVA, C. M. G.; FAUSTINO, L. R.; BRUNO, J. B.; MAGALHÃES, D. M.; CAMPELLO, C. C.; FIGUEIREDO, J. R.
Título: | Bovine serum albumin improves in vitro development of caprine preantral follicles.
Ano de publicação: | 2010
Fonte/Imprenta: | Animal Reproduction, v. 7, n. 4, p. 382-388, Oct./Dec. 2010.
Idioma: | Inglês
Conteúdo: | Abstract: This work investigated the effects of different protein supplements (fetal calf serum (FCS) and bovine serum albumin (BSA)) on the in vitro development of caprine preantral follicles. Preantral follicles (?150 µm) were isolated from ovarian cortex fragments and individually cultivated in ?-MEM medium in an incubator at 37°C for 24 days with 5% atmospheric CO2, and supplemented with either BSA at 1.25 or 3.0 mg/ml or FCS at 5 or 10%. An evaluation of follicular development was conducted based on survival rate, antrum formation, increase in follicular diameter, oocyte viability and obtainment of fully-grown oocytes. It was observed that from the 12th cultivation day, the percentage of surviving follicles under treatment with BSA at 3.0 mg/ml was greater than that of the other treatments (P < 0.05). As for follicular growth, although on the 6th day of cultivation FCS-treated follicles had a greater mean diameter (P < 0.05) when compared to those treated with BSA, on the last day of cultivation the mean diameter and antrum formation of follicles treated with BSA at 3.0 mg/ml were greater than those of follicles under other treatments (P < 0.05). With oocyte growth, the percentage of oocytes cultivated with BSA (3.0 mg/ml) that were destined for in vitro maturation (IVM; ?110 ?m diameter) was higher than that of other treatments. Moreover, under this treatment, 86% of oocytes presented a germinal vesicle and 14% restarted meiosis, out of which 3% were mature (metaphase II). In conclusion, supplementing cultivation medium with BSA at 3.0 mg/ml not only improves follicular development but also provides meioticallycompetent oocytes after in vitro cultivation of caprine preantral isolated follicles.
Palavras-Chave: | Folítulo pre-antral; Oócito; Soro-Albumina.
Thesagro: | Albumina; Cabra; Caprino; Cultura in vitro; Maturação; Reprodução animal.
Categoria do assunto: | --
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (27190-1) - ADD - AP - DD -  -  -  - DIS