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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
20/11/1992 |
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Data da última atualização: |
20/11/1992 |
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Autoria: |
EVERETT, N. P.; ROBINSON, K. E. P.; MASCARENHAS, D. |
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Afiliação: |
EniChem Americas, 2000 Princeton Park Corporate Center, Monmouth Junction, New Jersey 08852. |
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Título: |
Genetic engineering of sunflower (Helianthus annuus L.). |
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Ano de publicação: |
1987 |
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Fonte/Imprenta: |
Biotechnology, v.5, p.1201-1204, 1987. |
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Idioma: |
Inglês |
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Conteúdo: |
A disarmed derivate of the Ti:R772 cointegrate plasmid pAL969 was used to produce transgenic plants of a commercially important inbred line of oil-producing sunflower. A chimeric gene (neo) composed of the coding sequence of neomycin phosphotransferase (NPT) and 5' and 3' sequences from octopine synthase permitted the selection of kanamycin-resistant (Kan r) callus capable of plant regeneration kanamycin inhibited plant regeneration, precluding simultaneous plant regenation and selection for resistance to the antibiotic. Progeny analysis of transformed seedlings indicated Mendelian inheritance of neo. Expression of neo in transformed seedlings and plants indicated that the use of a Ti-derived promoter does not guarantee constitutive expression of the foreign gene. These results demonstrate that the introduction of foreign genes via Agrobacterium-mediated transformation can now be used for agronomic improvement of sunflower. |
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Palavras-Chave: |
Backcross; Sunflower. |
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Thesagro: |
Agrobacterium Tumefaciens; Engenharia Genética; Girassol; Helianthus Annuus; Retrocruzamento. |
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Thesaurus Nal: |
genetic engineering. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01581naa a2200241 a 4500
001 1457602
005 1992-11-20
008 1987 bl --- 0-- u #d
100 1 $aEVERETT, N. P.
245 $aGenetic engineering of sunflower (Helianthus annuus L.).
260 $c1987
520 $aA disarmed derivate of the Ti:R772 cointegrate plasmid pAL969 was used to produce transgenic plants of a commercially important inbred line of oil-producing sunflower. A chimeric gene (neo) composed of the coding sequence of neomycin phosphotransferase (NPT) and 5' and 3' sequences from octopine synthase permitted the selection of kanamycin-resistant (Kan r) callus capable of plant regeneration kanamycin inhibited plant regeneration, precluding simultaneous plant regenation and selection for resistance to the antibiotic. Progeny analysis of transformed seedlings indicated Mendelian inheritance of neo. Expression of neo in transformed seedlings and plants indicated that the use of a Ti-derived promoter does not guarantee constitutive expression of the foreign gene. These results demonstrate that the introduction of foreign genes via Agrobacterium-mediated transformation can now be used for agronomic improvement of sunflower.
650 $agenetic engineering
650 $aAgrobacterium Tumefaciens
650 $aEngenharia Genética
650 $aGirassol
650 $aHelianthus Annuus
650 $aRetrocruzamento
653 $aBackcross
653 $aSunflower
700 1 $aROBINSON, K. E. P.
700 1 $aMASCARENHAS, D.
773 $tBiotechnology$gv.5, p.1201-1204, 1987.
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Embrapa Soja (CNPSO) |
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| 1. |  | GUPTA, Y. K.; MARCELINO-GUIMARÃES, F. C.; LORRAIN, C.; FARMER, A.; HARIDAS, S.; FERREIRA, E. G. C.; LOPES-CAITAR, V. S.; OLIVEIRA, L. S.; MORIN, E.; WIDDISON, S.; CAMERON, C.; INOUE, Y.; THOR, K.; ROBINSON, K.; DRULA, E.; HENRISSAT, B.; LABUTTI, K.; BINI, A. M. R.; PAGET, E.; SINGAN, V.; DAUM, C.; DORME, C.; HOEK, M. V.; JANSSEN, A.; CHANDAT, L.; TARRIOTTE, Y.; RICHARDON, J.; MELO, B. do V. A.; WITTENBERG, A. H. J.; SCHNEIDERS, H.; PEYRARD, S.; ZANARDO, L. G.; HOLTMAN, V. C.; COULOMBIER-CHAUVEL, F.; LINK, T. I.; BALMER, D.; MÜLLER, A. N.; KIND, S.; BOHNERT, S.; WIRTZ, L.; CHEN, C.; YAN, M.; NG, V.; GAUTIER, P.; MEYER, M. C.; VOEGELE, R. T.; LIU, Q.; GRIGORIEV, I. V.; CONRATH, U.; BROMMONSCHENKEL, S. H.; LOEHRER, M.; SCHAFFRATH, U.; SIRVEN, C.; SCALLIET, G.; DUPLESSIS, S.; ESSE, H. P. van. Major proliferation of transposable elements shaped the genome of the soybean rust pathogen Phakopsora pachyrhizi. Nature Communications, v. 14, 1835, 2023. 16 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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