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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
23/03/2005 |
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Data da última atualização: |
19/06/2009 |
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Autoria: |
ONAL, A. G.; ROBINSON, J. J.; KURAN, M.; KING, M. E.; STAINES, M. E.; HUTCHINSON, J. S. M.; McEVOY, T. G. |
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Título: |
Effect of dietary supplementation of fish oil on the yield, quality and in vitro metabolism of embryos from superovulated sheep. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Theriogenology, v. 53, n. 1, p. 281, 2000. |
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Idioma: |
Inglês |
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Notas: |
Edição de proceedings Annual Conference of the International Embryo Transfer Society, Maastricht, The Netherlands, jan. 2000. |
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Conteúdo: |
Forty-eight mature Cheviot ewes were allocated randomly in equal numbers to a control diet based on equal parts of goats and sugar beet shreds or the same diet with 3% or 6% herring oil. The diets were given from 22 d before egg collection, which occurred 5 d after intrauterine AI. All ewes received a 12 d progestogen sponge (0.3 g Cronolone; Intervet, UK) and 9 mg oFSH (Ovagen, ICP, UK) in 8 equal injections every 12 h from 60 h before to 24 h after sponge removal. Ovulations and large follicles (>8 mm) were recorded at egg recovery via mid-line laparotomy. Embryos were cultured in SOF with 0.4% w/v BSA (5% CO2, 5% O2; 38.5°C) for up to 48 h; development in vitro was recorded every 24 h. After 48 h culture, cell nwnbers (nuclei counts; Hoechst 33342 stain) and protein synthesis (3H-phenylalanine incorporation into protein; cpm) in blastocysts were recorded. Estradiol concentrations were determined in plasma collected at Al. Dietary effects on ovulations and embryo yields (square-root transformed), blastocyst development and grade (arcsine-transformed), cell nwnbers and protein synthesis (log- transformed) and zona-intact diameters were tested by ANOVA. Ovulations and embryo yields were unaffected by dietary lipid but estradiol concentrations increased (P<0.05), and were associated with a tendency (P=0.08) towards more large follicles at egg collection. Supplementary fish oil tended to suppress the cate of embryo developrnent and reduced blastocyst diameter (P=0.07) and cell nwnber (P<0.05) but not protein synthesis. Funded by SOAEFD; AG Onal is supported by the Turkish Ministry of Education. MenosForty-eight mature Cheviot ewes were allocated randomly in equal numbers to a control diet based on equal parts of goats and sugar beet shreds or the same diet with 3% or 6% herring oil. The diets were given from 22 d before egg collection, which occurred 5 d after intrauterine AI. All ewes received a 12 d progestogen sponge (0.3 g Cronolone; Intervet, UK) and 9 mg oFSH (Ovagen, ICP, UK) in 8 equal injections every 12 h from 60 h before to 24 h after sponge removal. Ovulations and large follicles (>8 mm) were recorded at egg recovery via mid-line laparotomy. Embryos were cultured in SOF with 0.4% w/v BSA (5% CO2, 5% O2; 38.5°C) for up to 48 h; development in vitro was recorded every 24 h. After 48 h culture, cell nwnbers (nuclei counts; Hoechst 33342 stain) and protein synthesis (3H-phenylalanine incorporation into protein; cpm) in blastocysts were recorded. Estradiol concentrations were determined in plasma collected at Al. Dietary effects on ovulations and embryo yields (square-root transformed), blastocyst development and grade (arcsine-transformed), cell nwnbers and protein synthesis (log- transformed) and zona-intact diameters were tested by ANOVA. Ovulations and embryo yields were unaffected by dietary lipid but estradiol concentrations increased (P<0.05), and were associated with a tendency (P=0.08) towards more large follicles at egg collection. Supplementary fish oil tended to suppress the cate of embryo developrnent and reduced blastocyst diameter (P=0.07) and cell... Mostrar Tudo |
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Palavras-Chave: |
Óleo de peixe; Resposta ovariana; Suplementação. |
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Thesagro: |
Embrião; Nutrição; Ovino; Reprodução Animal; Superovulação. |
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Categoria do assunto: |
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Marc: |
LEADER 02585naa a2200301 a 4500
001 1531022
005 2009-06-19
008 2000 bl uuuu u00u1 u #d
100 1 $aONAL, A. G.
245 $aEffect of dietary supplementation of fish oil on the yield, quality and in vitro metabolism of embryos from superovulated sheep.
260 $c2000
500 $aEdição de proceedings Annual Conference of the International Embryo Transfer Society, Maastricht, The Netherlands, jan. 2000.
520 $aForty-eight mature Cheviot ewes were allocated randomly in equal numbers to a control diet based on equal parts of goats and sugar beet shreds or the same diet with 3% or 6% herring oil. The diets were given from 22 d before egg collection, which occurred 5 d after intrauterine AI. All ewes received a 12 d progestogen sponge (0.3 g Cronolone; Intervet, UK) and 9 mg oFSH (Ovagen, ICP, UK) in 8 equal injections every 12 h from 60 h before to 24 h after sponge removal. Ovulations and large follicles (>8 mm) were recorded at egg recovery via mid-line laparotomy. Embryos were cultured in SOF with 0.4% w/v BSA (5% CO2, 5% O2; 38.5°C) for up to 48 h; development in vitro was recorded every 24 h. After 48 h culture, cell nwnbers (nuclei counts; Hoechst 33342 stain) and protein synthesis (3H-phenylalanine incorporation into protein; cpm) in blastocysts were recorded. Estradiol concentrations were determined in plasma collected at Al. Dietary effects on ovulations and embryo yields (square-root transformed), blastocyst development and grade (arcsine-transformed), cell nwnbers and protein synthesis (log- transformed) and zona-intact diameters were tested by ANOVA. Ovulations and embryo yields were unaffected by dietary lipid but estradiol concentrations increased (P<0.05), and were associated with a tendency (P=0.08) towards more large follicles at egg collection. Supplementary fish oil tended to suppress the cate of embryo developrnent and reduced blastocyst diameter (P=0.07) and cell nwnber (P<0.05) but not protein synthesis. Funded by SOAEFD; AG Onal is supported by the Turkish Ministry of Education.
650 $aEmbrião
650 $aNutrição
650 $aOvino
650 $aReprodução Animal
650 $aSuperovulação
653 $aÓleo de peixe
653 $aResposta ovariana
653 $aSuplementação
700 1 $aROBINSON, J. J.
700 1 $aKURAN, M.
700 1 $aKING, M. E.
700 1 $aSTAINES, M. E.
700 1 $aHUTCHINSON, J. S. M.
700 1 $aMcEVOY, T. G.
773 $tTheriogenology$gv. 53, n. 1, p. 281, 2000.
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Embrapa Caprinos e Ovinos (CNPC) |
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| 1. |  | ANDRADE, J. B. de; BENINTENDE, R. P.; FERRARI JUNIOR, E.; PAULINO, V. T.; HENRIQUE, W.; WERNER, J. C.; MATTOS, H. B. de. Efeito das adubações nitrogenada e potássica na produção e composição da forragem de Brachiaria ruziziensis. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 31, n. 9, p. 617-620, set. 1996. Título em inglês: Effect of nitrogen and potash fertilizations on ruzigrass dry matter yield and composition.| Biblioteca(s): Embrapa Unidades Centrais. |
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