Data/hora: 09/06/2024 10:51:22

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	07/04/2004	
Data da última atualização: 	07/04/2004	
Autoria: 	ROA, A. C.; CHAVARRIAGA, P.; DUQUE, M. C.; BONIERBALE, M. W.; THOME, J.; GARY KOCHERT, C.	
Título: 	Microsatellites as a tool for assessing genetic diversity in Manihot species.	
Ano de publicação: 	1998	
Fonte/Imprenta: 	Revista Brasileira de Mandioca, Cruz das Almas, v. 17, p.17, nov, 1998. Suplemento.	
Idioma: 	Inglês	
Conteúdo: 	Abstract: Cassava and its wild relatives have been studied under different approaches, but there are still doubts about the possible ancestors of the crop and the level of variation present in the cultivated and the wild germplasm. Microsatellites or Simples Sequence Repeats (SSRS) have been widely recognized as powerful and informative genetic markers in plants and their utility results from their inherent variability even within populations. In order to evaluate the informativeness of these codominant markers in Manihot, a set of wild species that have been proposed as close relatives and a representative sample of the crop were assessed with 10 different primers pairs developed from cassava. In a samdle of 121 individuals were detected 124 alleles. From these, 79 were only in the wild taxa, indicating a greater alielic diversity than in the cultivated species. The numbre of alleles per locus ranged from 4 to 21 and the percentage of polynorphic loci varied from 57 up to 100. In general terms, microsatellite primers worked across the genus, but with increasing genetic distance there was a clear tendency for decreasing ability to successful amplify loci. Manihot aesculifolia, M. brachloba and M. carthaginensis did not present a banding paftern at 2 of the loci evaluted. A previous work with AFLP markers and the present STMS analysis showed that these 3 wild taxa are the most distant relatives to the crop, while the wild forms M. esculenta subsp. flabellifolia and M. esculenta subsp. peruviana appeared to be the most similar to Cassava. At the different loci, unique alleles were present mainly at the wild species. Microsatellite markers were particularly useful for showing a finescale differentiation within species.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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