| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Meio-Norte. |
|
Data corrente: |
29/12/1997 |
|
Data da última atualização: |
16/01/2024 |
|
Autoria: |
CAMPBELL, K. H. S.; McWHIR, J.; RITCHIE, W. A.; WILMUT, I. |
|
Título: |
Sheep cloned by nuclear transfer from a cultured cell line. |
|
Ano de publicação: |
1996 |
|
Fonte/Imprenta: |
Nature, v. 380, p. 64-66, Mar., 1996. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Nuclear transfer has been used in mammals as both a valuable tool in embryological studies and as a method for the multiplication of 'elite'embryos. offspring have only been reported when early embryos, or embryo-derived cells during primary culture, were used as nuclear donors. Here we provide the first report, to our knowledge, of live mammalian offspring following nuclear transfer from an established cell line. Lambs were born after cells derived from sheep embryos, which had been cultured for 6 to 13 passages, were induced to quiesce by serum starvation before transfer of their nuclei into enucleated oocytes. Induction of quiescence in the donor cells may modify the donor chromatin structure to help nuclear reprogramming and allow development. This approach will provide the same powerful opportunities for analysis and modification of gene function in livestock species that are available in the mouse through the use of embryonic stem cells. |
|
Palavras-Chave: |
Cloned; Nuclear. |
|
Thesagro: |
Clonagem; Ovino. |
|
Thesaurus Nal: |
sheep. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 01483naa a2200217 a 4500
001 1050166
005 2024-01-16
008 1996 bl uuuu u00u1 u #d
100 1 $aCAMPBELL, K. H. S.
245 $aSheep cloned by nuclear transfer from a cultured cell line.
260 $c1996
520 $aNuclear transfer has been used in mammals as both a valuable tool in embryological studies and as a method for the multiplication of 'elite'embryos. offspring have only been reported when early embryos, or embryo-derived cells during primary culture, were used as nuclear donors. Here we provide the first report, to our knowledge, of live mammalian offspring following nuclear transfer from an established cell line. Lambs were born after cells derived from sheep embryos, which had been cultured for 6 to 13 passages, were induced to quiesce by serum starvation before transfer of their nuclei into enucleated oocytes. Induction of quiescence in the donor cells may modify the donor chromatin structure to help nuclear reprogramming and allow development. This approach will provide the same powerful opportunities for analysis and modification of gene function in livestock species that are available in the mouse through the use of embryonic stem cells.
650 $asheep
650 $aClonagem
650 $aOvino
653 $aCloned
653 $aNuclear
700 1 $aMcWHIR, J.
700 1 $aRITCHIE, W. A.
700 1 $aWILMUT, I.
773 $tNature$gv. 380, p. 64-66, Mar., 1996.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio-Norte (CPAMN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 1 | |
| 1. |  | ARAGÃO, M. F.; FERNANDES, J. K. S.; MENESES, F. M. N.; ARAÚJO, R. A. de; PRIMO, A. A.; MARANHÃO, S. R.; VASCONCELOS, M. C. de; SOUZA, H. A. de. Leguminosas da caatinga como adubo verde em sorgo forrageiro cultivado em solo degradado. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 10., 2015, Teresina. [Anais...]. Teresina: Sociedade Nordestina de Produção Animal, 2015. 3 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
|    |
| Registros recuperados : 1 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
