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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
11/12/2000 |
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Data da última atualização: |
17/08/2023 |
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Autoria: |
HOOKEY, J. V.; RICHARDSON, J. F.; COOKSON, B. D. |
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Título: |
Molecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Journal of Clinical Microbiology, v. 36, n. 4, p. 1083-1089, Apr. 1998. |
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DOI: |
10.1128/JCM.36.4.1083-1089.1998. |
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Idioma: |
Inglês |
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Conteúdo: |
A typing procedure for Staphylococcus aureus was developed based on improved PCR amplification of the coagulase gene and restriction fragment length polymorphism (RFLP) analysis of the product. All coagulase-positive staphylococci produced a single PCR amplification product of either 875, 660, 603, or 547 bp. Those strains of epidemic methicillin-resistant S. aureus 16 (EMRSA-16) studied all gave a product of 547 bp. PCR products were digested withAluI and CfoI, and the fragments were separated by gel electrophoresis. Ten distinct RFLP patterns were found among 85 isolates of methicillin-resistant S. aureus (MRSA) and 10 propagating strains (PS) of methicillin-sensitive S. aureus(MSSA) examined. RFLP patterns 1, 2, and 3 were specific to strains of EMRSA-3, -15, and -16, respectively. By contrast, RFLP patterns 4 and 5 were seen with a heterogeneous collection of strains, together with drug-resistant forms of S. aureus isolated in Europe and four propagating strains used for the international phage set. RFLP pattern 6 was given by the Airedale isolate and PS 95. RFLP pattern 7 encompassed EMRSA-2 (isolate 331), PS 94, and PS 96. An isolate from Germany gave RFLP pattern 8. Eight strains of MSSA gave patterns similar to those of methicillin-resistant strains (RFLP patterns 3, 4, 5, 6, and 7), but two, PS 42E and PS 71, gave unique RFLP patterns 9 and 10, respectively. The coagulase gene PCR products for 24 isolates of MRSA and two isolates of MSSA were sequenced for both strands. The sequences were aligned, and evolutionary lineages were inferred based on pairwise distances between isolates. MenosA typing procedure for Staphylococcus aureus was developed based on improved PCR amplification of the coagulase gene and restriction fragment length polymorphism (RFLP) analysis of the product. All coagulase-positive staphylococci produced a single PCR amplification product of either 875, 660, 603, or 547 bp. Those strains of epidemic methicillin-resistant S. aureus 16 (EMRSA-16) studied all gave a product of 547 bp. PCR products were digested withAluI and CfoI, and the fragments were separated by gel electrophoresis. Ten distinct RFLP patterns were found among 85 isolates of methicillin-resistant S. aureus (MRSA) and 10 propagating strains (PS) of methicillin-sensitive S. aureus(MSSA) examined. RFLP patterns 1, 2, and 3 were specific to strains of EMRSA-3, -15, and -16, respectively. By contrast, RFLP patterns 4 and 5 were seen with a heterogeneous collection of strains, together with drug-resistant forms of S. aureus isolated in Europe and four propagating strains used for the international phage set. RFLP pattern 6 was given by the Airedale isolate and PS 95. RFLP pattern 7 encompassed EMRSA-2 (isolate 331), PS 94, and PS 96. An isolate from Germany gave RFLP pattern 8. Eight strains of MSSA gave patterns similar to those of methicillin-resistant strains (RFLP patterns 3, 4, 5, 6, and 7), but two, PS 42E and PS 71, gave unique RFLP patterns 9 and 10, respectively. The coagulase gene PCR products for 24 isolates of MRSA and two isolates of MSSA were sequenced for both stra... Mostrar Tudo |
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Palavras-Chave: |
Bacterial typing techniques; DNA bacterial; Methicillin Resistance. |
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Thesagro: |
Biologia Molecular; Microbiologia; Staphylococcus Aureus. |
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Thesaurus Nal: |
Coagulase positive staphylococci; Genotype; Polymerase chain reaction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02530naa a2200265 a 4500
001 1524190
005 2023-08-17
008 1998 bl uuuu u00u1 u #d
024 7 $a10.1128/JCM.36.4.1083-1089.1998.$2DOI
100 1 $aHOOKEY, J. V.
245 $aMolecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene.$h[electronic resource]
260 $c1998
520 $aA typing procedure for Staphylococcus aureus was developed based on improved PCR amplification of the coagulase gene and restriction fragment length polymorphism (RFLP) analysis of the product. All coagulase-positive staphylococci produced a single PCR amplification product of either 875, 660, 603, or 547 bp. Those strains of epidemic methicillin-resistant S. aureus 16 (EMRSA-16) studied all gave a product of 547 bp. PCR products were digested withAluI and CfoI, and the fragments were separated by gel electrophoresis. Ten distinct RFLP patterns were found among 85 isolates of methicillin-resistant S. aureus (MRSA) and 10 propagating strains (PS) of methicillin-sensitive S. aureus(MSSA) examined. RFLP patterns 1, 2, and 3 were specific to strains of EMRSA-3, -15, and -16, respectively. By contrast, RFLP patterns 4 and 5 were seen with a heterogeneous collection of strains, together with drug-resistant forms of S. aureus isolated in Europe and four propagating strains used for the international phage set. RFLP pattern 6 was given by the Airedale isolate and PS 95. RFLP pattern 7 encompassed EMRSA-2 (isolate 331), PS 94, and PS 96. An isolate from Germany gave RFLP pattern 8. Eight strains of MSSA gave patterns similar to those of methicillin-resistant strains (RFLP patterns 3, 4, 5, 6, and 7), but two, PS 42E and PS 71, gave unique RFLP patterns 9 and 10, respectively. The coagulase gene PCR products for 24 isolates of MRSA and two isolates of MSSA were sequenced for both strands. The sequences were aligned, and evolutionary lineages were inferred based on pairwise distances between isolates.
650 $aCoagulase positive staphylococci
650 $aGenotype
650 $aPolymerase chain reaction
650 $aBiologia Molecular
650 $aMicrobiologia
650 $aStaphylococcus Aureus
653 $aBacterial typing techniques
653 $aDNA bacterial
653 $aMethicillin Resistance
700 1 $aRICHARDSON, J. F.
700 1 $aCOOKSON, B. D.
773 $tJournal of Clinical Microbiology$gv. 36, n. 4, p. 1083-1089, Apr. 1998.
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Embrapa Caprinos e Ovinos (CNPC) |
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| 43. |  | RIBEIRO, M. de F. Biologia e manejo de abelhas sem ferrão. In: SIMPÓSIO DE PRODUÇÃO ANIMAL DO VALE DO SÃO FRANCISCO, 2., 2009, Petrolina. Anais... Petrolina: Univasf: Embrapa Semi-Árido, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
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| 47. | | RIBEIRO, R. P. Cooperação e competição na dinâmica do desenvolvimento tecnológico. In: CONGRESSO NACIONAL DE MILHO E SORGO, 23., 2000, Uberlândia, MG. A inovação tecnológica e a competividade no contexto dos mercados globalizados: resumos expandidos. Sete Lagoas: ABMS: Embrapa Milho e Sorgo; Uberlândia: Universidade Federal de Uberlandia, 2000. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
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| 55. | | PLOTEGHER, F.; RIBEIRO, C. Caracterização do fertilizante superfosfato simples - um estudo sobre o efeito da moagem sobre a cinética de solubilidade. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 8, 2014, Juiz de fora. Anais... São Carlos: Embrapa Instrumentação; Campo Grande: Embrapa Gado de Corte; Juiz de Fora: Embrapa Gado de Leite, 2014. p. 395-399. Editores: Maria Alice Martins, Humberto de Mello Brandão, Marlene de Barros Coelho, Daniel Souza Corrêa, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.| Tipo: Artigo em Anais de Congresso |
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| 57. | | RIBEIRO, S. I. Citros no Estado do Pará. In: EMBRAPA AMAZÔNIA ORIENTAL. Programa de melhoramento genético e de adaptação de espécies vegetais para a Amazônia Oriental. Belém, PA, 1999. p. 29-36. (Embrapa Amazônia Oriental. Documentos, 16).| Biblioteca(s): Embrapa Amazônia Oriental. |
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