Data/hora: 13/06/2024 01:45:56

Biblioteca(s): | Embrapa Soja.
Data corrente: | 29/03/2016
Data da última atualização: | 29/03/2016
Autoria: | HELENE, L. C. F.; DELAMUTA, J. R. M.; RIBEIRO. R. A.; ORMEÑO-ORRILLO, E.; ROGEL, M. A; MARTINEZ-ROMERO, E.; HUNGRIA, M.
Afiliação: | Luisa Caroline Ferraz Helene, UEL; Jakeline Renata Marçon Delamuta, UEL; Renan Augusto Ribeiro, Conselho Nacional de Desenvolvimento Cientifico e Tecnológico; Ernesto Ormeño-Orrillo, Universidad Nacional Agraria La Molina; Marco Antonio Rogel, Centro de Ciencias Genó micas, Universidad Nacional Autónoma de México; Esperanza Martinez-Romero, Centro de Ciências Genómicas, Universidad Nacional Autónoma de México; MARIANGELA HUNGRIA DA CUNHA, CNPSO.
Título: | Bradyrhizobium viridifuturi sp. nov., encompassing nitrogen-fixing symbionts of legumes used for green manure and environmental services.
Ano de publicação: | 2015
Fonte/Imprenta: | International Journal of Systematic and Evolutionary Microbiology, Reading v. 65, n. 12, 2015.
Páginas: | p. 4441-4438
Idioma: | Português
Conteúdo: | Symbiotic nitrogen-fixing bacteria, commonly called rhizobia, are agronomically important because they can provide significant amounts of nitrogen to plants and help in recovery of impoverished soils and improvement of degraded environments. In recent years, with advances in molecular techniques, several studies have shown that these bacteria have high levels of genetic diversity, resulting in taxonomic reclassifications and descriptions of new species. However, despite the advances achieved, highly conserved 16S ribosomal genes (16S rRNA) do not elucidate differences between species of several genera, including the genus Bradyrhizobium. Other methodologies, such as multilocus sequence analysis (MLSA), have been used in such cases, with good results. In this study, three strains (SEMIAs 690T, 6387 and 6428) of the genus Bradyrhizobium, isolated from nitrogen-fixing nodules of Centrosema and Acacia species, without clear taxonomic positions, were studied. These strains differed from genetically closely related species according to the results of MLSA of four housekeeping genes (dnaK, glnII, gyrB and recA) and nucleotide identities of the concatenated genes with those of related species ranged from 87.8 % to 95.7 %, being highest with Bradyrhizobium elkanii. DNA?DNA hybridization (less than 32 % DNA relatedness) and average nucleotide identity values of the whole genomes (less than 90.5 %) indicated that these strains represented a novel species, and phenotypic traits were determined. Our data supported the description of the SEMIA strains as Bradyrhizobium viridifuturi sp. nov., and SEMIA 690T (5CNPSo 991T5C 100aT5BR 1804T5LMG 28866T), isolated from Centrosema pubescens, was chosen as type strain.
Palavras-Chave: | Fixação biologica de nitrogenio.
Categoria do assunto: | --
Registro original: | Embrapa Soja (CNPSO)
Exemplares: | CNPSO - (36671-1) - UPC - AP - DD -  -  -  - DIS