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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
18/03/1999 |
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Data da última atualização: |
09/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAGÃO, F. J. L.; RIBEIRO, S. G.; BARROS, L. M. G.; BRASILEIRO, A. C. M.; MAXWELL, D. P.; RECH, E. L.; FARIA, J. C. |
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Afiliação: |
FRANCISCO JOSE LIMA ARAGAO, Cenargen; SIMONE DA GRACA RIBEIRO, Cenargen; LEILA MARIA GOMES BARROS, Cenargen; ANA CRISTINA MIRANDA BRASILEIRO, Cenargen; UNIVERSITY OF WISCONSIN; ELIBIO LEOPOLDO RECH FILHO, Cenargen; JOSIAS CORREA DE FARIA, CNPAF. |
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Título: |
Transgenic beans (Phaseolus vulgaris L.) engineered to express viral antisense RNAs show delayed and attenuated symptoms to bean golden mosaic geminivirus. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Molecular Breeding, v. 4, n. 6, p. 491-499, Dec. 1998. |
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DOI: |
https://doi.org/10.1023/A:1009613607559 |
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Idioma: |
Inglês |
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Conteúdo: |
The genes Rep-TrAP-REn and BC1 from the Brazilian isolate bean golden mosaic geminivirus (BGMV-BR) were cloned in antisense orientation under the transcriptional control of the CaMV 35S promoter. This construct was used to transform common bean (Phaseolus vulgaris L.) using the biolistic method. Transgenic plants from the R3 and R4 generations were challenged by inoculation with a BGMV-BR viruliferous whitefly population. Of the four transgenic lines tested, two had both delayed and attenuated viral symptoms. Un-transformed plants or plants transformed with a construct containing only the gus-neo gene developed typical BGMV-BR symptoms 10?15 days after inoculation. |
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Palavras-Chave: |
Bean; BGMV; Bombardment; Common; Particle; Plant; Resistance; Transformacao; Transformation. |
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Thesagro: |
Feijão; Phaseolus Vulgaris; Planta; Resistência; Vírus. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01722naa a2200373 a 4500
001 1205638
005 2022-05-09
008 1998 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1023/A:1009613607559$2DOI
100 1 $aARAGÃO, F. J. L.
245 $aTransgenic beans (Phaseolus vulgaris L.) engineered to express viral antisense RNAs show delayed and attenuated symptoms to bean golden mosaic geminivirus.
260 $c1998
520 $aThe genes Rep-TrAP-REn and BC1 from the Brazilian isolate bean golden mosaic geminivirus (BGMV-BR) were cloned in antisense orientation under the transcriptional control of the CaMV 35S promoter. This construct was used to transform common bean (Phaseolus vulgaris L.) using the biolistic method. Transgenic plants from the R3 and R4 generations were challenged by inoculation with a BGMV-BR viruliferous whitefly population. Of the four transgenic lines tested, two had both delayed and attenuated viral symptoms. Un-transformed plants or plants transformed with a construct containing only the gus-neo gene developed typical BGMV-BR symptoms 10?15 days after inoculation.
650 $aFeijão
650 $aPhaseolus Vulgaris
650 $aPlanta
650 $aResistência
650 $aVírus
653 $aBean
653 $aBGMV
653 $aBombardment
653 $aCommon
653 $aParticle
653 $aPlant
653 $aResistance
653 $aTransformacao
653 $aTransformation
700 1 $aRIBEIRO, S. G.
700 1 $aBARROS, L. M. G.
700 1 $aBRASILEIRO, A. C. M.
700 1 $aMAXWELL, D. P.
700 1 $aRECH, E. L.
700 1 $aFARIA, J. C.
773 $tMolecular Breeding$gv. 4, n. 6, p. 491-499, Dec. 1998.
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