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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/04/2019 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SABINO, Y. N. V.; FOCHAT, R. C.; LIMA, J. C. F.; RIBEIRO, M. T.; ARCURI, P. B.; CARNEIRO, J. da C.; MACHADO, M. A.; REIS, D. R. de L.; MACHADO, A. B. F.; HÚNGARO, H. M.; RIBEIRO, J. B.; PAIVA, A. D. |
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Afiliação: |
Yasmin Neves Vieira Sabino, UFJF; Romário Costa Fochat, UFJF; JUNIOR CESAR FERNANDES LIMA, CNPGL; MARLICE TEIXEIRA RIBEIRO, CNPGL; PEDRO BRAGA ARCURI, CNPGL; JAILTON DA COSTA CARNEIRO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; Alessandra Barbosa Ferreira Machado, UFTM, Uberaba; Humberto Moreira Húngaro, UFJF; JOAO BATISTA RIBEIRO, CNPGL; Aline Dias Paiva, UFTM, Uberaba. |
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Título: |
Antibacterial activity and lantibiotic post-translational modification genes in Streptococcus spp. isolated from ruminal fluid. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Annals of Microbiology, v. 69, p. 131-138, 2019. |
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DOI: |
10.1007/s13213-018-1407-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract The production of bacteriocins is frequently described in high microbial diversity environments. The aims of this study were to screen Streptococcus spp. isolated from rumen for their antibacterial potential and to determine the presence of post-translational modification genes for lantibiotic class of bacteriocins. The isolates were tested for production of antibacterial compounds by the spot-on-lawn assay. Presence of interfering factors and the sensitivity to proteinase K were evaluated. The ruminal bacteria were identified by 16S rRNA gene sequencing and the subspecific discrimination of the isolates belonging to the same specie was performed by PFGE. The presence of lantibiotic post-translational modification genes (lanB, lanC, and lanM) into bacterial genomes was performed by PCR. The bacteriocin-like inhibitory substances showed broad inhibitory activity and the producer cells were identified as S. equinus, S. lutetiensis, and S. gallolyticus. According to PFGE, the isolates identified as S. equinus belong to different strains. Three ruminal isolates showed at least one of the lantibiotic post-translational modification genes, and lanC was more frequently detected (75%). The production of broad-spectrum bacteriocin-like inhibitory substances by rumen strains suggests that antimicrobial peptides may play an important role in competition in the complex ruminal ecosystem. |
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Palavras-Chave: |
Antibacterial activity; Bacteriocin; LanC; Lantibiotic; Streptococcus sp. |
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Thesagro: |
Rúmen. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02399naa a2200337 a 4500
001 2108297
005 2023-01-24
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1007/s13213-018-1407-2$2DOI
100 1 $aSABINO, Y. N. V.
245 $aAntibacterial activity and lantibiotic post-translational modification genes in Streptococcus spp. isolated from ruminal fluid.$h[electronic resource]
260 $c2019
520 $aAbstract The production of bacteriocins is frequently described in high microbial diversity environments. The aims of this study were to screen Streptococcus spp. isolated from rumen for their antibacterial potential and to determine the presence of post-translational modification genes for lantibiotic class of bacteriocins. The isolates were tested for production of antibacterial compounds by the spot-on-lawn assay. Presence of interfering factors and the sensitivity to proteinase K were evaluated. The ruminal bacteria were identified by 16S rRNA gene sequencing and the subspecific discrimination of the isolates belonging to the same specie was performed by PFGE. The presence of lantibiotic post-translational modification genes (lanB, lanC, and lanM) into bacterial genomes was performed by PCR. The bacteriocin-like inhibitory substances showed broad inhibitory activity and the producer cells were identified as S. equinus, S. lutetiensis, and S. gallolyticus. According to PFGE, the isolates identified as S. equinus belong to different strains. Three ruminal isolates showed at least one of the lantibiotic post-translational modification genes, and lanC was more frequently detected (75%). The production of broad-spectrum bacteriocin-like inhibitory substances by rumen strains suggests that antimicrobial peptides may play an important role in competition in the complex ruminal ecosystem.
650 $aRúmen
653 $aAntibacterial activity
653 $aBacteriocin
653 $aLanC
653 $aLantibiotic
653 $aStreptococcus sp
700 1 $aFOCHAT, R. C.
700 1 $aLIMA, J. C. F.
700 1 $aRIBEIRO, M. T.
700 1 $aARCURI, P. B.
700 1 $aCARNEIRO, J. da C.
700 1 $aMACHADO, M. A.
700 1 $aREIS, D. R. de L.
700 1 $aMACHADO, A. B. F.
700 1 $aHÚNGARO, H. M.
700 1 $aRIBEIRO, J. B.
700 1 $aPAIVA, A. D.
773 $tAnnals of Microbiology$gv. 69, p. 131-138, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Cutter |
Registro |
Volume |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
25/04/2023 |
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Data da última atualização: |
25/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
TOCHETTO, C.; JUNQUEIRA, D. M.; ANDERSON, T. K.; GAVA, D.; HAACH, V.; CANTAO, M. E.; BAKER, A. L. V.; SCHAEFER, R. |
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Afiliação: |
DANIELLE GAVA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; REJANE SCHAEFER, CNPSA. |
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Título: |
Introductions of human-origin Seasonal H3N2, H1N2 and pre-2009 H1N1 Influenza Viruses to swine in Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Viruses, v. 15, n. 576, 2023. |
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DOI: |
https://doi.org/10.3390/v15020576 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: In South America, the evolutionary history of influenza A virus (IAV) in swine has been obscured by historically low levels of surveillance, and this has hampered the assessment of the zoonotic risk of emerging viruses. The extensive genetic diversity of IAV in swine observed globally has been attributed mainly to bidirectional transmission between humans and pigs. We conducted surveillance in swine in Brazil during 2011?2020 and characterized 107 H1N1, H1N2, and H3N2 IAVs. Phylogenetic analysis based on HA and NA segments revealed that human seasonal IAVs were introduced at least eight times into swine in Brazil since the mid-late 1980s. Our analyses revealed three genetic clades of H1 within the 1B lineage originated from three distinct spillover events, and an H3 lineage that has diversified into three genetic clades. The N2 segment from human seasonal H1N2 and H3N2 viruses was introduced into swine six times and a single introduction of an N1 segment from the human H1N1 virus was identified. Additional analysis revealed further reassortment with H1N1pdm09 viruses. All these introductions resulted in IAVs that apparently circulate only in Brazilian herds. These results reinforce the significant contributions of human IAVs to the genetic diversity of IAV in swine and reiterate the importance of surveillance of IAV in pigs. |
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Palavras-Chave: |
Genetic diversity; Reassortment; Spillover. |
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Thesagro: |
Genética Animal; Suíno; Variação Genética. |
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Thesaurus NAL: |
Disease surveillance; Influenza A virus; Swine. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1153351/1/final10094.pdf
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Marc: |
LEADER 02273naa a2200325 a 4500
001 2153351
005 2023-04-25
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/v15020576$2DOI
100 1 $aTOCHETTO, C.
245 $aIntroductions of human-origin Seasonal H3N2, H1N2 and pre-2009 H1N1 Influenza Viruses to swine in Brazil.$h[electronic resource]
260 $c2023
520 $aAbstract: In South America, the evolutionary history of influenza A virus (IAV) in swine has been obscured by historically low levels of surveillance, and this has hampered the assessment of the zoonotic risk of emerging viruses. The extensive genetic diversity of IAV in swine observed globally has been attributed mainly to bidirectional transmission between humans and pigs. We conducted surveillance in swine in Brazil during 2011?2020 and characterized 107 H1N1, H1N2, and H3N2 IAVs. Phylogenetic analysis based on HA and NA segments revealed that human seasonal IAVs were introduced at least eight times into swine in Brazil since the mid-late 1980s. Our analyses revealed three genetic clades of H1 within the 1B lineage originated from three distinct spillover events, and an H3 lineage that has diversified into three genetic clades. The N2 segment from human seasonal H1N2 and H3N2 viruses was introduced into swine six times and a single introduction of an N1 segment from the human H1N1 virus was identified. Additional analysis revealed further reassortment with H1N1pdm09 viruses. All these introductions resulted in IAVs that apparently circulate only in Brazilian herds. These results reinforce the significant contributions of human IAVs to the genetic diversity of IAV in swine and reiterate the importance of surveillance of IAV in pigs.
650 $aDisease surveillance
650 $aInfluenza A virus
650 $aSwine
650 $aGenética Animal
650 $aSuíno
650 $aVariação Genética
653 $aGenetic diversity
653 $aReassortment
653 $aSpillover
700 1 $aJUNQUEIRA, D. M.
700 1 $aANDERSON, T. K.
700 1 $aGAVA, D.
700 1 $aHAACH, V.
700 1 $aCANTAO, M. E.
700 1 $aBAKER, A. L. V.
700 1 $aSCHAEFER, R.
773 $tViruses$gv. 15, n. 576, 2023.
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