Data/hora: 23/06/2024 21:05:52

Biblioteca(s): | Embrapa Semiárido.
Data corrente: | 08/07/2015
Data da última atualização: | 03/05/2017
Autoria: | PINTO, M. dos S. T.; RIBEIRO, J. M.; ARAUJO, F. P. de; MELO, N. F. de; FERNANDES, K. V. S.
Afiliação: | MARCIO DOS SANTOS TEIXEIRA PINTO, Universidade Federal do Tocantins - UFT; JULIANA MARTINS RIBEIRO, CPATSA; FRANCISCO PINHEIRO DE ARAUJO, CPATSA; NATONIEL FRANKLIN DE MELO, CPATSA; KÁTIA VALEVSKI SALES FERNANDES, Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF.
Título: | Purification and characterization of a peroxidase present in xilopodium exsudates of umbu plants (Spondias tuberosa A.).
Ano de publicação: | 2015
Fonte/Imprenta: | African Journal of Biotechnology, v. 14, n. 21, p. 1838-1845, 2015.
DOI: | 10.5897/AJB2015.14521
Idioma: | Inglês
Conteúdo: | Umbu plants are drought resistant trees which are able to store water and several other substances into its adapted root, named xylopodium. The exsudate from xilopodium is a natural solution rich in salts, sugars and a little concentration of proteins. In this work, we report the purification of a peroxidase (POX) from umbu xilopodium exsudate by direct extraction from polyacrylamide electrophoresis gels. Umbu POX showed optimum activity at pHs around 6.0 to 7.0 and high thermal resistance after incubation at 7 0°C for 6 min. POX activity present in crude extracts was more heat - resistant than in its purified form. When assayed with metal ions, umbu POX activity was shown to be inhibited by Mn 2+ and stimulated by Ca 2+ and Mg 2+ ; it was also inhibited by sodium azid e (concentrations higher then 1 mM) and not inhibited by either EDTA or tropolone. POX Km values for guaiacol and methylcatechol substrates were 6.83 and 22.25, respectively. The enzyme is proposed to be a guaiacol peroxidase and was seen to be located at higher concentrations in the outermost layers of xylopodium tissue, such as the endoderm.
Palavras-Chave: | Guaiacol peroxidase; Root enzyme; Spondia tuberosa; Tssue brownig.
Thesagro: | Caatinga; Peroxidase; Umbu.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/126327/1/Juliana-2015.pdf
Registro original: | Embrapa Semiárido (CPATSA)
Exemplares: | CPATSA - (54798-1) - UPC - AP - DD -  -  -  - DIS