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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria Tropical; Embrapa Gado de Leite. |
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Data corrente: |
09/12/2019 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MENDONÇA, J. F. M. DE; VIEIRA, F. DE O.; FONSECA, I.; RIBEIRO, J. B.; ARCURI, E. F.; BORGES, M. DE F.; BORGES, C. A. V.; SÁ, J. F. O. DE; MARTINS, M. F. |
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Afiliação: |
JULIANA FRANÇA MONTEIRO DE MENDONÇA, Universidade Federal de Juiz de Fora; FELIPE DE OLIVEIRA VIEIRA, Universidade Federal de Juiz de Fora; ISABELA FONSECA, Instituto Federal Catarinense, Videira, SC; JOAO BATISTA RIBEIRO, CNPGL; EDNA FROEDER ARCURI, CNPGL; MARIA DE FATIMA BORGES, Embrapa Agroindústria Tropical, Doutora, Pesquisadora aposentada; CRISTIANO AMANCIO VIEIRA BORGES, CNPGL; JAQUELINE FLAVIANA OLIVEIRA DE SÁ, Empresa de Pesquisa Agropecuária de Minas Gerais; MARTA FONSECA MARTINS, CNPGL. |
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Título: |
Detection of viable Salmonella Typhimurium and Staphylococcus aureus in coalho cheese by real-time PCR. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Food Science and Technology, Campinas, v. 39, p. 690-696, out. 2019. |
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DOI: |
https://doi.org/10.1590/fst.29318 |
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Idioma: |
Inglês |
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Conteúdo: |
Real-time PCR (qPCR) has been used for rapid identification of Salmonella Typhimurium and Staphylococcus aureus in dairy foods, but is unable to differentiate viable and unviable pathogens. Ethidium bromide monoazide (EMA), a DNA-intercalating agent, can detect only viable cells because selectively enter cells considered unviable and bind to their DNA, inhibiting its amplification during qPCR. The objective was to establish a protocol for detection of viable Salmonella Typhimurium and S. aureus, experimentally inoculated in coalho cheese, by the use of EMA combined with qPCR. The protocol was effective for the identification of viable Salmonella Typhimurium in coalho cheese but not for the S. aureus cells. Concentrations of viable Salmonella Typhimurium cells of 1 CFU/10 g of coalho cheese could be detected. The monoplex protocol enables the rapid and specific identification of viable Salmonella Typhimurium in coalho cheese, making it an alternative method for the quality and safety control of cheeses. |
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Palavras-Chave: |
Agentes intercalantes de DNA; Doenças transmitidas por alimentos; Lacticínios; Microrganismos patogênicos; Viabilidade celular. |
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Thesaurus Nal: |
Cell viability; Dairy products. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1118145/1/Detection-of-viable-Salmonella-Typhimurium-and-Staphylococcus.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/206473/1/ART19051.pdf
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Marc: |
LEADER 02019naa a2200313 a 4500
001 2118145
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/fst.29318$2DOI
100 1 $aMENDONÇA, J. F. M. DE
245 $aDetection of viable Salmonella Typhimurium and Staphylococcus aureus in coalho cheese by real-time PCR.$h[electronic resource]
260 $c2019
520 $aReal-time PCR (qPCR) has been used for rapid identification of Salmonella Typhimurium and Staphylococcus aureus in dairy foods, but is unable to differentiate viable and unviable pathogens. Ethidium bromide monoazide (EMA), a DNA-intercalating agent, can detect only viable cells because selectively enter cells considered unviable and bind to their DNA, inhibiting its amplification during qPCR. The objective was to establish a protocol for detection of viable Salmonella Typhimurium and S. aureus, experimentally inoculated in coalho cheese, by the use of EMA combined with qPCR. The protocol was effective for the identification of viable Salmonella Typhimurium in coalho cheese but not for the S. aureus cells. Concentrations of viable Salmonella Typhimurium cells of 1 CFU/10 g of coalho cheese could be detected. The monoplex protocol enables the rapid and specific identification of viable Salmonella Typhimurium in coalho cheese, making it an alternative method for the quality and safety control of cheeses.
650 $aCell viability
650 $aDairy products
653 $aAgentes intercalantes de DNA
653 $aDoenças transmitidas por alimentos
653 $aLacticínios
653 $aMicrorganismos patogênicos
653 $aViabilidade celular
700 1 $aVIEIRA, F. DE O.
700 1 $aFONSECA, I.
700 1 $aRIBEIRO, J. B.
700 1 $aARCURI, E. F.
700 1 $aBORGES, M. DE F.
700 1 $aBORGES, C. A. V.
700 1 $aSÁ, J. F. O. DE
700 1 $aMARTINS, M. F.
773 $tFood Science and Technology, Campinas$gv. 39, p. 690-696, out. 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
03/11/2022 |
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Data da última atualização: |
27/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 3 |
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Autoria: |
MARINHO, J. de S. R.; VALDISSER, P. A. M. R.; BRONDANI, C.; PAVANELLI, I.; VIANELLO, R. P. |
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Afiliação: |
JULIANA DE SOUZA RODRIGUES MARINHO, UNIVERSIDADE FEDERAL DE GOIÁS; PAULA ARIELLE M RIBEIRO VALDISSER, CNPAF; CLAUDIO BRONDANI, CNPAF; ISABELA PAVANELLI, UNIVERSIDADE FEDERAL DE GOIÁS; ROSANA PEREIRA VIANELLO, CNPAF. |
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Título: |
Molecular markers for assessing the inter- and intra-racial genetic diversity and structure of common bean. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Genetic Resources and Crop Evolution, v. 70, n. 1, p. 263-279, Jan. 2023. |
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ISSN: |
0925-9864 |
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DOI: |
https://doi.org/10.1007/s10722-022-01432-4 |
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Idioma: |
Inglês |
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Notas: |
Early access July 2022. |
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Conteúdo: |
The Mesoamerican and Andean gene pools of the common bean are distinguished into specific races. Knowledge of their structure and genetic diversity is an important prerequisite for plant conservation and breeding. The aim of this study was to estimate the inter- and intra-racial genetic diversity of genotypes representative of the six common bean races using simple sequence repeats (SSRs) and single nucleotide polymorphism (SNPs). Ninety-four accessions were genetically analyzed, using 24 SSR and 4.820 SNPs markers with minor allele frequencies (MAF)>- 5%. Based on SSR, 163 alleles were identified (13.8 alleles/marker). For the whole samples, the average values for expected (He) and observed heterozygosity (Ho) were 0.780 and 0.045, respectively, and 5.5 × 10-31 for probability of identity (PI). While for the gene pools analyzed separately, similar He estimates were obtained for the Andean (He = 0.731) and Mesoamerican gene pools (He = 0.733). Population structure and clustering analyses revealed subdivision predominantly by gene pool (K = 2). The overall linkage disequilibrium extension (r2sv) was 58 kb. Estimated He was 0.327 and Ho 0.025, with slightly higher values for the Mesoamerican (He= 0.303) when compared to its Andean counterpart (He = 0.269). Population structure was observed in the Andean (K = 2 and K = 3) and Mesoamerican (K = 2) gene pools. The molecular analyses did not reveal clear differentiation between the bean races. Although sampling may be having an effect on the analysis, our results reinforce that distinction among races appeared to have resulted from selection caused by domestication. MenosThe Mesoamerican and Andean gene pools of the common bean are distinguished into specific races. Knowledge of their structure and genetic diversity is an important prerequisite for plant conservation and breeding. The aim of this study was to estimate the inter- and intra-racial genetic diversity of genotypes representative of the six common bean races using simple sequence repeats (SSRs) and single nucleotide polymorphism (SNPs). Ninety-four accessions were genetically analyzed, using 24 SSR and 4.820 SNPs markers with minor allele frequencies (MAF)>- 5%. Based on SSR, 163 alleles were identified (13.8 alleles/marker). For the whole samples, the average values for expected (He) and observed heterozygosity (Ho) were 0.780 and 0.045, respectively, and 5.5 × 10-31 for probability of identity (PI). While for the gene pools analyzed separately, similar He estimates were obtained for the Andean (He = 0.731) and Mesoamerican gene pools (He = 0.733). Population structure and clustering analyses revealed subdivision predominantly by gene pool (K = 2). The overall linkage disequilibrium extension (r2sv) was 58 kb. Estimated He was 0.327 and Ho 0.025, with slightly higher values for the Mesoamerican (He= 0.303) when compared to its Andean counterpart (He = 0.269). Population structure was observed in the Andean (K = 2 and K = 3) and Mesoamerican (K = 2) gene pools. The molecular analyses did not reveal clear differentiation between the bean races. Although sampling may be having an ef... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genética; Genetic diversity; SNP genotyping; SSR genotyping. |
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Thesagro: |
Feijão; Genética Molecular; Marcador Molecular; Raça. |
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Thesaurus NAL: |
Beans; Molecular genetics; Races; Single nucleotide polymorphism. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02711naa a2200349 a 4500
001 2147995
005 2023-09-27
008 2023 bl uuuu u00u1 u #d
022 $a0925-9864
024 7 $ahttps://doi.org/10.1007/s10722-022-01432-4$2DOI
100 1 $aMARINHO, J. de S. R.
245 $aMolecular markers for assessing the inter- and intra-racial genetic diversity and structure of common bean.$h[electronic resource]
260 $c2023
500 $aEarly access July 2022.
520 $aThe Mesoamerican and Andean gene pools of the common bean are distinguished into specific races. Knowledge of their structure and genetic diversity is an important prerequisite for plant conservation and breeding. The aim of this study was to estimate the inter- and intra-racial genetic diversity of genotypes representative of the six common bean races using simple sequence repeats (SSRs) and single nucleotide polymorphism (SNPs). Ninety-four accessions were genetically analyzed, using 24 SSR and 4.820 SNPs markers with minor allele frequencies (MAF)>- 5%. Based on SSR, 163 alleles were identified (13.8 alleles/marker). For the whole samples, the average values for expected (He) and observed heterozygosity (Ho) were 0.780 and 0.045, respectively, and 5.5 × 10-31 for probability of identity (PI). While for the gene pools analyzed separately, similar He estimates were obtained for the Andean (He = 0.731) and Mesoamerican gene pools (He = 0.733). Population structure and clustering analyses revealed subdivision predominantly by gene pool (K = 2). The overall linkage disequilibrium extension (r2sv) was 58 kb. Estimated He was 0.327 and Ho 0.025, with slightly higher values for the Mesoamerican (He= 0.303) when compared to its Andean counterpart (He = 0.269). Population structure was observed in the Andean (K = 2 and K = 3) and Mesoamerican (K = 2) gene pools. The molecular analyses did not reveal clear differentiation between the bean races. Although sampling may be having an effect on the analysis, our results reinforce that distinction among races appeared to have resulted from selection caused by domestication.
650 $aBeans
650 $aMolecular genetics
650 $aRaces
650 $aSingle nucleotide polymorphism
650 $aFeijão
650 $aGenética Molecular
650 $aMarcador Molecular
650 $aRaça
653 $aDiversidade genética
653 $aGenetic diversity
653 $aSNP genotyping
653 $aSSR genotyping
700 1 $aVALDISSER, P. A. M. R.
700 1 $aBRONDANI, C.
700 1 $aPAVANELLI, I.
700 1 $aVIANELLO, R. P.
773 $tGenetic Resources and Crop Evolution$gv. 70, n. 1, p. 263-279, Jan. 2023.
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Embrapa Arroz e Feijão (CNPAF) |
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