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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
11/09/2017 |
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Data da última atualização: |
06/04/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMPOS, C. G.; VERAS, H. C. T.; RIBEIRO, J. A. de A.; COSTA, P. P. K. G.; ARAÚJO, K. P.; RODRIGUES, C. M.; ALMEIDA, J. R. M. de; ABDELNUR, P. V. |
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Afiliação: |
CHRISTIANE GONÇALVES CAMPOS, Universidade Federal de Goiás; HENRIQUE CÉSAR TEIXEIRA VERAS, Universidade de Brasília; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; PATRICIA PINTO KALIL G COSTA, CNPAE; KATIÚSCIA PEREIRA ARAÚJO; CLENILSON MARTINS RODRIGUES, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE. |
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Título: |
New protocol based on UHPLC-MS/MS for quantitation of metabolites in xylose-fermenting yeasts. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of the American Society for Mass Spectrometry, v. 28, n. 12, p. 2646-2657, 2017. |
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Páginas: |
p. 2646-2657 |
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DOI: |
10.1007/s13361-017-1786-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Xylose fermentation is a bottleneck in second-generation ethanol production. As such, a comprehensive understanding of xylose metabolism in naturally xylose-fermenting yeasts is essential for prospection and construction of recombinant yeast strains. The objective of the current study was to establish a reliable metabolomics protocol for quantification of key metabolites of xylose catabolism pathways in yeast, and to apply this protocol to Spathaspora arborariae. Ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was used to quantify metabolites, and afterwards, sample preparation was optimized to examine yeast intracellular metabolites. S. arborariae was cultivated using xylose as a carbon source under aerobic and oxygen-limited conditions. Ion pair chromatography (IPC) and hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) were shown to efficiently quantify 14 and 5 metabolites, respectively, in a more rapid chromatographic protocol than previously described. Thirteen and eleven metabolites were quantified in S. arborariae under aerobic and oxygen-limited conditions, respectively. This targeted metabolomics protocol is shown here to quantify a total of 19 metabolites, including sugars, phosphates, coenzymes, monosaccharides, and alcohols, from xylose catabolism pathways (glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle) in yeast. Furthermore, to our knowledge, this is the first time that intracellular metabolites have been quantified in S. arborariae after xylose consumption. The results indicated that fine control of oxygen levels during fermentation is necessary to optimize ethanol production by S. arborariae. The protocol presented here may be applied to other yeast species and could support yeast genetic engineering to improve second generation ethanol production. Graphical Abstract ?. MenosAbstract: Xylose fermentation is a bottleneck in second-generation ethanol production. As such, a comprehensive understanding of xylose metabolism in naturally xylose-fermenting yeasts is essential for prospection and construction of recombinant yeast strains. The objective of the current study was to establish a reliable metabolomics protocol for quantification of key metabolites of xylose catabolism pathways in yeast, and to apply this protocol to Spathaspora arborariae. Ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was used to quantify metabolites, and afterwards, sample preparation was optimized to examine yeast intracellular metabolites. S. arborariae was cultivated using xylose as a carbon source under aerobic and oxygen-limited conditions. Ion pair chromatography (IPC) and hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) were shown to efficiently quantify 14 and 5 metabolites, respectively, in a more rapid chromatographic protocol than previously described. Thirteen and eleven metabolites were quantified in S. arborariae under aerobic and oxygen-limited conditions, respectively. This targeted metabolomics protocol is shown here to quantify a total of 19 metabolites, including sugars, phosphates, coenzymes, monosaccharides, and alcohols, from xylose catabolism pathways (glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle) in yeast. Furthermore, to our knowledge, this is... Mostrar Tudo |
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Palavras-Chave: |
Spathaspora arborariae; UHPLC-MS/MS; Xylose fermentation. |
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Thesaurus Nal: |
Mass spectrometry; Metabolomics; Xylose. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163703/1/Abdelnur-ASMassSoectiometry-2017.pdf
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Marc: |
LEADER 02860naa a2200301 a 4500
001 2075378
005 2021-04-06
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1007/s13361-017-1786-9$2DOI
100 1 $aCAMPOS, C. G.
245 $aNew protocol based on UHPLC-MS/MS for quantitation of metabolites in xylose-fermenting yeasts.$h[electronic resource]
260 $c2017
300 $ap. 2646-2657
520 $aAbstract: Xylose fermentation is a bottleneck in second-generation ethanol production. As such, a comprehensive understanding of xylose metabolism in naturally xylose-fermenting yeasts is essential for prospection and construction of recombinant yeast strains. The objective of the current study was to establish a reliable metabolomics protocol for quantification of key metabolites of xylose catabolism pathways in yeast, and to apply this protocol to Spathaspora arborariae. Ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was used to quantify metabolites, and afterwards, sample preparation was optimized to examine yeast intracellular metabolites. S. arborariae was cultivated using xylose as a carbon source under aerobic and oxygen-limited conditions. Ion pair chromatography (IPC) and hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) were shown to efficiently quantify 14 and 5 metabolites, respectively, in a more rapid chromatographic protocol than previously described. Thirteen and eleven metabolites were quantified in S. arborariae under aerobic and oxygen-limited conditions, respectively. This targeted metabolomics protocol is shown here to quantify a total of 19 metabolites, including sugars, phosphates, coenzymes, monosaccharides, and alcohols, from xylose catabolism pathways (glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle) in yeast. Furthermore, to our knowledge, this is the first time that intracellular metabolites have been quantified in S. arborariae after xylose consumption. The results indicated that fine control of oxygen levels during fermentation is necessary to optimize ethanol production by S. arborariae. The protocol presented here may be applied to other yeast species and could support yeast genetic engineering to improve second generation ethanol production. Graphical Abstract ?.
650 $aMass spectrometry
650 $aMetabolomics
650 $aXylose
653 $aSpathaspora arborariae
653 $aUHPLC-MS/MS
653 $aXylose fermentation
700 1 $aVERAS, H. C. T.
700 1 $aRIBEIRO, J. A. de A.
700 1 $aCOSTA, P. P. K. G.
700 1 $aARAÚJO, K. P.
700 1 $aRODRIGUES, C. M.
700 1 $aALMEIDA, J. R. M. de
700 1 $aABDELNUR, P. V.
773 $tJournal of the American Society for Mass Spectrometry$gv. 28, n. 12, p. 2646-2657, 2017.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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| Registros recuperados : 110 | |
| 61. |  | CAMPOS, C.; RIBEIRO, J. A. de A.; COSTA, P. P. K. G.; VERAS, H.; ALMEIDA, J. R. M. de; RODRIGUES, C. M.; ABDELNUR, P. V. Metabolomics analysis of xylose-fermenting yeasts, Spathaspora arborariae and Candida tenuis, using UHPLC-MS/MS. In: IBERO-AMERICAN, 1.; BrMASS CONFERENCE ON MASS SPECTROMETRY, 6., 2016, Rio de Janeiro. Resumos Eletrônicos... São Paulo: Sociedade Brasileira de Espectrometria de Massas, 2016. p. 237-238.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 62. | | VARGAS. L. H. G.; RIBEIRO, J. A. de A.; SIFUENTES, D. N.; OLIVEIRA, A. E.; SOUZA JUNIOR, M. T.; RODRIGUES, C. M.; ABDELNUR, P. V. Metabolomics of elaeis guineensis leaves: from optimization of the sample preparation steps to untarged metabolite profiling alidis. In: ASMS CONFERENCE ON MASS SPECTROMETRY AND ALLIED TOPICS, 61., 2013, Minneapolis. [Proceedings...]. Santa Fe: American Society for Mass Spectrometry, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 63. | | CAMPOS, C. G.; VERAS, H. C. T.; RIBEIRO, J. A. de A.; COSTA, P. P. K. G.; RODRIGUES, C. M.; ALMEIDA, J. R. M. de; ABDELNUR, P. V. Preparo de amostra em metabolômica de leveduras para a determinação dos metabólitos produzidos na fermentação de xilose. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 2., 2015, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2015. p. 81-83| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
|  |
| 65. | | RODRIGUES NETO, J. C.; CORREIA, M. V.; SOUTO, A. L.; RIBEIRO, J. A. de A.; RODRIGUES, C. M.; ABDELNUR, P. V. Validation of a PLS-DA Model for biomarker discovery in Elaeis guineensis leaves related to fatal yellowing using UHPLC-MS/MS analysis. In: WORLD CHEMISTRY CONGRESS, 46.,; REUNIÃO ANNUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 40., 2017, São Paulo, SP. [Resumos ...]. São Paulo: SBQ, 2017. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 66. | | CONCEIÇÃO, A. A.; ARAÚJO, A. P. F.; SANTANA, T. D. D.; RIBEIRO, J. A. de A.; MENDONCA, S.; SIQUEIRA, F. G. de. Validation of a RP-UPLC-PDA method for gossypol determination in cottonseed meal during biodetoxification process by fungi. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 2., 2015, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2015. p. 13-14| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 67. | | GUIMARÃES, M. B.; PORTO, B. L. S.; RIBEIRO, J. A. de A.; CARMONA, P. A. O.; CUNHA, R. N. V. da; MENDONCA, S. Variação dos parâmetros associados ao rendimento e qualidade de óleo no período pós-colheita do dendê híbrido. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 3., 2016, Brasília, DF. Anais ... Brasília, DF: Embrapa, 2016. p. 229-236.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 68. | | GUIMARÃES, M. B.; PORTO, B. L. S.; RIBEIRO, J. A. de A.; CARMONA, P. A. O.; CUNHA, R. N. V. da; MENDONCA, S. Variação dos parâmetros associados ao rendimento e qualidade de óleo no período pós-colheita do dendê híbrido. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 3., 2016, Brasília, DF. Anais ... Brasília, DF: Embrapa, 2016. p. 229-236.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 69. | | SILVA, C. de O. G.; TEIXEIRA, T. S.; RIBEIRO, J. A. de A.; RODRIGUES, K. B.; ANASTÁCIO, G. S.; SOUZA, A. A.; MENDES, T. D.; SALUM, T. F. C.; FAVARO, L. C. de L.; ABDELNUR, P. V. avaliação da atividade da LPMO Cel61A de trichoderma reesei sobre celulose por espectrometria de massas. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 25.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 70. | | SOUTO, A. L.; ARAÚJO, K. P. de; SILVA, R. M. C. da; POLETTO, C. M.; RIBEIRO, J. A. de A.; DAMASO, M. C. T.; RODRIGUES, C. M.; ABDELNUR, P. V. Aplicação da análise multivariada de dados de Espectrometria de Massas em amostras de glicerol biotransformadas por fungos filamentosos In: CONGRESSO BRASILEIRO DE QUÍMICA, 55., 2015, Goiânia. Recursos renováveis: inovação e tecnologia. Rio de Janeiro: ABQ, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 71. | | CAMPOS, C. G.; RIBEIRO, J. A. de A.; COSTA, P. P. K. G.; PEREIRA, K. A.; VERAS, H. C.; ALMEIDA, J. R. M. de; RODRIGUES, C. M.; ABDELNUR, P. V. Análise quantitativa dos metabólitos produzidos por levedura na via de fermentação da xilose utilizando UHPLC-MS/MS. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 39., 2016, Goiânia, GO. Criar e empreender: anais ... São Paulo: SBQ, 2016. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 72. | | BRAGA, I. de O.; SILVA, T. L. C. da; SILVA, T. L. C. da; RODRIGUES NETO, J. C.; RIBEIRO, J. A. de A.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Characterization of the response of Gliricidia Sepium (JACQ.) Steud. To saline stress through the use of proteomic and metabolomic. In: BRAZILIAN CONGRESS OF GENETICS, 67., 2022, Natal, RN. Porto Alegre: Sociedade Brasileira de Genética, 2022. p. 281 e-book| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 73. | | BRAGA, I. de O.; SILVA, T. L. C. da; SILVA, T. L. C. da; RODRIGUES NETO, J. C.; RIBEIRO, J. A. de A.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Characterization of the response of Gliricidia Sepium (JACQ.) Steud. To saline stress through the use of proteomic and metabolomic. In: BRAZILIAN CONGRESS OF GENETICS, 67., 2022, Natal, RN. p. 281 e-book| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 74. | | PEREIRA, C. B.; SILVA, R. M. C. da; POLETTO, C. M.; CAMPOS, C. G.; RIBEIRO, J. A. de A.; RODRIGUES, C. M.; ABDELNUR, P. V.; ALMEIDA, J. R. M. de; DAMASO, M. C. T. Screening of microorganisms for production of aggregated-value chemicals from glycerin bioconversion. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 37., 2015, San Diego, California. [Resumos ...] Fairfax: Society for Industrial Microbiology and Biotechnology, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 75. | | MOURA, L. T. S.; SOUZA, D. P. M.; MENDONCA, S.; RIBEIRO, J. A. de A.; SOUSA, L. F.; RAMOS, A. T.; MAIORKA, P. C.; ARAÚJO, V. L.; MARUO, V. M. Histopathological and Reproductive Evaluation in Male Rats Fed Jatropha curcas Seed Cake with or without Alkaline Hydrolysis and Subjected to Heat Treatment. BioMed Research International, v. 2017, artigo 6123408 , 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 76. | | CAMPOS, C. G.; RIBEIRO, J. A. de A.; VERAS, H. C. T.; COSTA, P. P. K. G.; ARAÚJO, K. P.; ALMEIDA, J. R. M. de; RODRIGUES, C. M.; ABDELNUR, P. V. Identificação de alvos moleculares na via metabólica de fermentação de xilose utilizando estratégias de metabolômica. In: WORKSHOP DA PÓS-GRADUAÇÃO EM QUÍMICA, 1., 2015, Goiânia, GO. [Resumos ...] Goiânia: UFG, 2015. WSPGQ-027| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 77. | | BITTENCOURT, C. B.; SILVA, T. L. C. da; RODRIGUES NETO, J. C.; VIEIRA, L. R.; LEAO, A. P.; RIBEIRO, J. A. de A.; ABDELNUR, P. V.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Insights from a multi-omics integration (MOI) study in oil palm (Elaeis gineensis Jacq.) response to abiotic stresses: part one - salinity. Plants, 11, n. 1755, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Agroenergia; Embrapa Meio-Norte. |
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| 78. | | LEAO, A. P.; BITTENCOURT, C. B.; SILVA, T. L. C. da; RODRIGUES NETO, J. C.; BRAGA, I. de O.; VIEIRA, L. R.; RIBEIRO, J. A. de A.; SOUSA, C. A. F. de; ABDELNUR, P. V.; SOUZA JUNIOR, M. T. Insights from a Multi-Omics Integration (MOI) Study in Oil Palm (Elaeis gineensis Jacq.) Response to Abiotic Stresses: Part Two - Drought. Plants, 11, n. 2786, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 79. | | LEAO, A. P.; BITTENCOURT, C. B.; SILVA, T. L. C. da; RODRIGUES NETO, J. C.; BRAGA, Í. de O.; VIEIRA, L. R.; RIBEIRO, J. A. de A.; ABDELNUR, P. V.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Insights from a Multi-Omics Integration (MOI) study in Oil Palm (Elaeis guineensis Jacq.) response to abiotic stresses: Part Two-Drought. Plants, v. 11, n. 20, 2786, Oct. 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 80. | | BITTENCOURT, C. B.; SILVA, T. L. C. da; RODRIGUES NETO, J. C.; VIEIRA, L. R.; LEAO, A. P.; RIBEIRO, J. A. de A.; ABDELNUR, P. V.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Insights from a Multi-Omics Integration (MOI) study in oil palm (ELAEIS GUINEENSIS JACQ.) response to salinity and drought stresses. In: BRAZILIAN CONGRESS OF GENETICS, 67., 2022, Natal, RN. Porto Alegre: Sociedade Brasileira de Genética, 2022. p. 394| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 110 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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