Data/hora: 21/06/2024 16:24:27

Biblioteca(s): | Embrapa Agroenergia.
Data corrente: | 15/03/2019
Data da última atualização: | 06/05/2019
Autoria: | CAMPOS, C. G.; RIBEIRO, J. A. de A.; ALMEIDA, J. R. M. de; QUIRINO, B. F.; ABDELNUR, P. V.
Afiliação: | Christiane Gonçalves Campos; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE; BETANIA FERRAZ QUIRINO, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE.
Título: | Targeted metabolomics of Xylose-Fermenting yeasts based on mass spectrometry.
Ano de publicação: | 2019
Fonte/Imprenta: | In: BAIDOO E. (Eds.) Microbial metabolomics. Methods in Molecular Biology. New York, NY: Humana Press, 2019.
Volume: | 1859.
Idioma: | Português
Conteúdo: | Mass spectrometry is a sensitive and selective analytical technique that enables detection and quantitation of low abundance compounds in a complex sample matrix. Targeted metabolomics allows for quantitative analysis of metabolites, providing kinetic information of production and consumption rates, an essential step to investigate microbial metabolism. Here, we describe a targeted metabolomics protocol for yeast samples, from sample preparation to mass spectrometry analysis, which enables the identification of metabolic fluxes after xylose consumption. Sample preparation methods were optimized for quenching of yeast metabolism followed by intracellular metabolite extraction, using cold methanol and boiling ethanol protocols. Ultrahigh performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) methods using ion pair chromatography (IPC) and hydrophilic interaction liquid chromatography (HILIC) allowed for the quantitation of 18 metabolites involved in central carbon metabolism (glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle). The protocol here described was successfully applied to quantify metabolites in Scheffersomyces stipitis, Spathaspora passalidarum, Spathaspora arborariae, and Candida tenuis samples after xylose consumption.
Palavras-Chave: | Microbial metabolomics.
Thesaurus Nal: | Biotechnology; Yeasts.
Categoria do assunto: | --
Registro original: | Embrapa Agroenergia (CNPAE)
Exemplares: | CNPAE - (3442-1) - UPC - PL - DD -  -  -  - DIS