Data/hora: 23/09/2024 02:07:10

Biblioteca(s): 	Embrapa Recursos Genéticos e Biotecnologia.	
Data corrente: 	06/01/2016	
Data da última atualização: 	20/06/2024	
Autoria: 	FREIRE, M. S.; CANTUÁRIA, A. P. C.; LIMA, S. M. F.; ALMEIDA, J. A.; MURAD, A. M.; FRANCO, O. L.; REZENDE, T. M. B.	
Afiliação: 	MIRNA S. FREIRE, UCB; ANA PAULA C. CANTUÁRIA, UnB; STELLA M. F. LIMA, UCB; JEESER A. ALMEIDA, UCB; ANDRE MELRO MURAD, CENARGEN; OCTAVIO L. FRANCO, UCB; TAIA M. B. REZENDE, UCB.	
Título: 	NanoUPLC-MS(E) proteomic analysis of osteoclastogenesis downregulation by IL-4.	
Ano de publicação: 	2015	
Fonte/Imprenta: 	Journal of Proteomics, v. 131, p. 8-16, 2015.	
Idioma: 	Português	
Conteúdo: 	Bone resorption is an important factor in bone homeostasis and imbalance can cause several diseases. In osteoimmunology, IL-4 has been described as an important factor in promoting M2 macrophage profile. In order to shed some light on the effect of IL-4 on osteoclast precursors in the presence of RANKL, cytokines and nitric oxide (NO) production and the proteomic profile were analyzed. The presence of IL-4 in in vitro osteoclastogenesis provides production of TNF-?, IL-1?, IL-1?, IL-10 and IL-12 at basal cell levels. Regarding NO production, IL-4 was sufficient to increase the basal NO levels. Proteomic analyses identified 877 global proteins. IL-4 in in vitro RANKL-mediated osteoclastogenesis leads to the expression of 118 proteins. The presence of rIL-4 in in vitro rRANKL-mediated-osteoclastogenesis downregulated this process. However, the proteomics findings in the osteoclastogenesis demonstrated a much greater effect on osteoclast precursor cells than on RANKL-differentiated osteoclasts. These results suggest that the main effect of IL-4 in pre-osteoclast cells leads to a M2 macrophage activation, and this probably contributed to a reduction in osteoclastogenesis when both stimuli were used. This study noticed that IL-4 plays an important regulatory role in bone homeostasis due to its suppressive potential of precursor osteoclast cells.	
Palavras-Chave: 	NanoUPLC-MSE; Osteoimmunology.	
Thesaurus Nal: 	bone resorption; interleukin-4.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)	
Exemplares: 	CENARGEN - (36146-1) - UPC - AP - DD -  -  -  - DIS