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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
07/03/1997 |
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Data da última atualização: |
07/03/1997 |
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Autoria: |
REDMER, D. A.; DAI, Y.; LI, J.; CHARNOCK-JONES, D.S.; SMITH, S. K.; REYNOLDS, L. P.; MOOR, R. M. |
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Título: |
Characterization and expression of vascular endothelial growth factor (VEGF) in the ovine corpus luteum. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Journal of Reproduction and Fertility, v.108, n.1, p.157-165, 1996. |
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Idioma: |
Inglês |
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Conteúdo: |
The corpus luteum undergoes tremendous growth, development and regression each, oestrous or menstrual cycle. These changes are reflected by equally impressive growth and regression of the luteal vasculature. We have previously shown that angiogenic factors from corpora lutea are primarily heparin binding and that one of these factors is similar to vascular endothelial growth factor (VEGF). ln an effort to identify this factor, and to define its role in luteal vascular development, the cDNA for the coding region of ovine VEGF was sequenced and a sensitive RNase protection assay was developed to quantitate mRNA encoding VEGF in luteal tissues from ewes in the early (days 2-4), mid- (day 8) and late (days 14-15) stages of the oestrous cycle. ln addition, an N-terminal peptide was synthesized from the translated ovine cDNA sequence for VEGF and an antiserum was raised against this peptide for use in western immunoblotting procedures. Nested reverse transcriptase (RT)-PCR of RNA from ovine corpora lutea resulted in three products that correspond in size to the alternatively spliced variants of VEGF (VEGF120 VEGF164 and VEGF188) predicted from other species. The RNase protection assay revealed that the proportion of mRNA encoding VEGF was 2- to 3-fold greater on days 2-4 than on clay 8 or days 14-15. Densitometric analysis of gels from the RNase protection assay showed that VEGF 120 represented approximately one third of the total mRNA encoding VEGF in the corpus luteum and that this proportion did not vary with stage of the oestrous cycle. SDS-PAGE and western immunoblot analysis of a homogenate from corpora lutea showed a single 18 kDa protein. These data demonstrate that VEGF is expressed in luteal tissue throughout the ovine oestrous cycle and that expression of mRNA encoding VEGF is upregulated during the period of rapid luteal development, when luteal vascular growth is at its maximum. MenosThe corpus luteum undergoes tremendous growth, development and regression each, oestrous or menstrual cycle. These changes are reflected by equally impressive growth and regression of the luteal vasculature. We have previously shown that angiogenic factors from corpora lutea are primarily heparin binding and that one of these factors is similar to vascular endothelial growth factor (VEGF). ln an effort to identify this factor, and to define its role in luteal vascular development, the cDNA for the coding region of ovine VEGF was sequenced and a sensitive RNase protection assay was developed to quantitate mRNA encoding VEGF in luteal tissues from ewes in the early (days 2-4), mid- (day 8) and late (days 14-15) stages of the oestrous cycle. ln addition, an N-terminal peptide was synthesized from the translated ovine cDNA sequence for VEGF and an antiserum was raised against this peptide for use in western immunoblotting procedures. Nested reverse transcriptase (RT)-PCR of RNA from ovine corpora lutea resulted in three products that correspond in size to the alternatively spliced variants of VEGF (VEGF120 VEGF164 and VEGF188) predicted from other species. The RNase protection assay revealed that the proportion of mRNA encoding VEGF was 2- to 3-fold greater on days 2-4 than on clay 8 or days 14-15. Densitometric analysis of gels from the RNase protection assay showed that VEGF 120 represented approximately one third of the total mRNA encoding VEGF in the corpus luteum and that... Mostrar Tudo |
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Thesagro: |
Corpo Lúteo; Fator de Crescimento; Ovino; Reprodução. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02605naa a2200241 a 4500
001 1516483
005 1997-03-07
008 1996 bl uuuu u00u1 u #d
100 1 $aREDMER, D. A.
245 $aCharacterization and expression of vascular endothelial growth factor (VEGF) in the ovine corpus luteum.
260 $c1996
520 $aThe corpus luteum undergoes tremendous growth, development and regression each, oestrous or menstrual cycle. These changes are reflected by equally impressive growth and regression of the luteal vasculature. We have previously shown that angiogenic factors from corpora lutea are primarily heparin binding and that one of these factors is similar to vascular endothelial growth factor (VEGF). ln an effort to identify this factor, and to define its role in luteal vascular development, the cDNA for the coding region of ovine VEGF was sequenced and a sensitive RNase protection assay was developed to quantitate mRNA encoding VEGF in luteal tissues from ewes in the early (days 2-4), mid- (day 8) and late (days 14-15) stages of the oestrous cycle. ln addition, an N-terminal peptide was synthesized from the translated ovine cDNA sequence for VEGF and an antiserum was raised against this peptide for use in western immunoblotting procedures. Nested reverse transcriptase (RT)-PCR of RNA from ovine corpora lutea resulted in three products that correspond in size to the alternatively spliced variants of VEGF (VEGF120 VEGF164 and VEGF188) predicted from other species. The RNase protection assay revealed that the proportion of mRNA encoding VEGF was 2- to 3-fold greater on days 2-4 than on clay 8 or days 14-15. Densitometric analysis of gels from the RNase protection assay showed that VEGF 120 represented approximately one third of the total mRNA encoding VEGF in the corpus luteum and that this proportion did not vary with stage of the oestrous cycle. SDS-PAGE and western immunoblot analysis of a homogenate from corpora lutea showed a single 18 kDa protein. These data demonstrate that VEGF is expressed in luteal tissue throughout the ovine oestrous cycle and that expression of mRNA encoding VEGF is upregulated during the period of rapid luteal development, when luteal vascular growth is at its maximum.
650 $aCorpo Lúteo
650 $aFator de Crescimento
650 $aOvino
650 $aReprodução
700 1 $aDAI, Y.
700 1 $aLI, J.
700 1 $aCHARNOCK-JONES, D.S.
700 1 $aSMITH, S. K.
700 1 $aREYNOLDS, L. P.
700 1 $aMOOR, R. M.
773 $tJournal of Reproduction and Fertility$gv.108, n.1, p.157-165, 1996.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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