| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Uva e Vinho. |
|
Data corrente: |
11/06/2019 |
|
Data da última atualização: |
06/01/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
FALAVIGNA, V. da S.; MALABARBA, J.; SILVEIRA, C. P.; BUFFON, V.; MARIATH, J. E. de A.; PASQUALI, G.; MARGIS-PINHEIRO, M.; REVERS, L. F. |
|
Afiliação: |
Vítor da Silveira Falavigna, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, Brazil; Jaiana Malabarba, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, BraziL; Carolina Pereira Silveira, Embrapa Uva e Vinho, Rua Livramento, 515, P.O. Box 130, Bento Gonçalves, RS 95701-008, Brazil; VANESSA BUFFON, CNPUV; Jorge Ernesto de Araújo Mariath, Laboratory of Plant Anatomy, Botany Department, UFRGS, Porto Alegre 91501-970, Brazil; Giancarlo Pasquali, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, Brazil; Márcia Margis?Pinheiro, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, Brazil; LUIS FERNANDO REVERS, CNPUV. |
|
Título: |
Characterization of the nucellus-specific dehydrin MdoDHN11 demonstrates its involvement in the tolerance to water deficit. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Plant Cell Reports [online], 2019. |
|
DOI: |
10.1007/s00299-019-02428-8 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract Key message MdoDHN11 acts in the nucellus layer to protect the embryo and the endosperm from limited water availability during apple seed development.AbstractDehydrins (DHNs) are protective proteins related to several plant developmental responses that involve dehy-dration such as seed desiccation and abiotic stresses. In apple (Malus × domestica Borkh.), the seed-specific MdoDHN11was suggested to play important roles against dehydration during seed development. However, this hypothesis has not yet been evaluated. Within this context, several experiments were performed to functionally characterize MdoDHN11. In situ hybridization analysis during apple seed development showed that MdoDHN11 expression is confined to a maternal tissue called nucellus, a central mass of parenchyma between the endosperm and the testa. The MdoDHN11 protein was local-ized in the cytosol and nucleus. Finally, transgenic Arabidopsis plants expressing MdoDHN11 were generated and exposed to a severe water-deficit stress, aiming to mimic a situation that can occurs during seed development. All transgenic lines showed increased tolerance to water deficit in relation to wild-type plants. Taken together, our results provide evidences that MdoDHN11 plays important roles during apple seed development by protecting the embryo and the endosperm from limited water availability, and the mechanism of action probably involves the interaction of MdoDHN11 with proteins and other components in the cell.Keywords Apple · Dehydrin · Seed development · Transgenic expression · Water-deficit stress MenosAbstract Key message MdoDHN11 acts in the nucellus layer to protect the embryo and the endosperm from limited water availability during apple seed development.AbstractDehydrins (DHNs) are protective proteins related to several plant developmental responses that involve dehy-dration such as seed desiccation and abiotic stresses. In apple (Malus × domestica Borkh.), the seed-specific MdoDHN11was suggested to play important roles against dehydration during seed development. However, this hypothesis has not yet been evaluated. Within this context, several experiments were performed to functionally characterize MdoDHN11. In situ hybridization analysis during apple seed development showed that MdoDHN11 expression is confined to a maternal tissue called nucellus, a central mass of parenchyma between the endosperm and the testa. The MdoDHN11 protein was local-ized in the cytosol and nucleus. Finally, transgenic Arabidopsis plants expressing MdoDHN11 were generated and exposed to a severe water-deficit stress, aiming to mimic a situation that can occurs during seed development. All transgenic lines showed increased tolerance to water deficit in relation to wild-type plants. Taken together, our results provide evidences that MdoDHN11 plays important roles during apple seed development by protecting the embryo and the endosperm from limited water availability, and the mechanism of action probably involves the interaction of MdoDHN11 with proteins and other components in the cell.Keywor... Mostrar Tudo |
|
Palavras-Chave: |
Transgenic expression; Water-deficit stress. |
|
Thesaurus Nal: |
Apples; Dehydrins; Seed development. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198444/1/Falavigna2019-Article-CharacterizationOfTheNucellus-.pdf
|
|
Marc: |
LEADER 02425naa a2200277 a 4500
001 2109788
005 2020-01-06
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1007/s00299-019-02428-8$2DOI
100 1 $aFALAVIGNA, V. da S.
245 $aCharacterization of the nucellus-specific dehydrin MdoDHN11 demonstrates its involvement in the tolerance to water deficit.$h[electronic resource]
260 $c2019
520 $aAbstract Key message MdoDHN11 acts in the nucellus layer to protect the embryo and the endosperm from limited water availability during apple seed development.AbstractDehydrins (DHNs) are protective proteins related to several plant developmental responses that involve dehy-dration such as seed desiccation and abiotic stresses. In apple (Malus × domestica Borkh.), the seed-specific MdoDHN11was suggested to play important roles against dehydration during seed development. However, this hypothesis has not yet been evaluated. Within this context, several experiments were performed to functionally characterize MdoDHN11. In situ hybridization analysis during apple seed development showed that MdoDHN11 expression is confined to a maternal tissue called nucellus, a central mass of parenchyma between the endosperm and the testa. The MdoDHN11 protein was local-ized in the cytosol and nucleus. Finally, transgenic Arabidopsis plants expressing MdoDHN11 were generated and exposed to a severe water-deficit stress, aiming to mimic a situation that can occurs during seed development. All transgenic lines showed increased tolerance to water deficit in relation to wild-type plants. Taken together, our results provide evidences that MdoDHN11 plays important roles during apple seed development by protecting the embryo and the endosperm from limited water availability, and the mechanism of action probably involves the interaction of MdoDHN11 with proteins and other components in the cell.Keywords Apple · Dehydrin · Seed development · Transgenic expression · Water-deficit stress
650 $aApples
650 $aDehydrins
650 $aSeed development
653 $aTransgenic expression
653 $aWater-deficit stress
700 1 $aMALABARBA, J.
700 1 $aSILVEIRA, C. P.
700 1 $aBUFFON, V.
700 1 $aMARIATH, J. E. de A.
700 1 $aPASQUALI, G.
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aREVERS, L. F.
773 $tPlant Cell Reports [online], 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Uva e Vinho (CNPUV) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 174 | |
| 121. |  | RITSCHEL, P. S.; REVERS, L. F.; LEITE, G. B.; FERREIRA, M. E.; OLIVEIRA, P. R. D. de. Genetic analysis of accessions in a pear germplasm bank. In: INTERNATIONAL PEAR SYMPOSIUM, 10., 2007, Peniche. Programme and abstracts. [Alcobaça, Portugal: ISHS], 2007. p. 40. Resumo PO 37.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
|    |
| 122. | | MALABARBA, J.; BUFFON, V.; WAIRICH, A.; GAETA, M. L.; MARIATH, J. E. A.; DORNELAS, M. C.; REVERS, L. F. Gene expression analysis of VVAG3 in grapevine reproductive tissues by in situ hybridization. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: Sociedade Brasileira de Genética, 2013. p. 3. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 124. | | MALABARBA, J.; BUFFON, V.; MARIATH, J. E. A.; MARASHIN, F. S.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. Manipulation of VviAGL11 expression changes the seed content in grapevine (Vitis vinifera L.). Plant Science, v. 269, p. 126-135, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 125. | | GASPERIN, A. C.; ANZANELLO, R.; PERINI, P.; REVERS, L. F.; FIALHO, F. B.; SANTOS, H. P. dos. Influência das gemas laterais sobre a gema apical em ramos de macieira da cv. Castel Gala. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 8.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 4., 2010, Bento Gonçalves. Resumos. Bento Gonçalves: Embrapa Uva e Vinho, 2010. p. p. 41. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 126. | | COSTENARO-DA-SILVA, D.; PASSAIA, G.; HENRIQUES, J. A. P.; MARGIS, R.; PASQUALI, G.; REVERS, L. F. Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine. Plant Science, Limerick, v. 179, n. 5, p. 510-519, nov. 2010. 179 510-519| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 127. | | COSTENARO-DA-SILVA, D.; PASSAIA, G.; HENRIQUES, J. A. P.; MARGIS, R.; PASQUALI, G.; REVERS, L. F. Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine. In: CONGRESSO BRASILEIRO DE GENÉTICA, 56., 2010, Guarujá. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2010. p. 206. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 128. | | MIOTTO, Y. E.; FALAVIGNA, V. da S.; PORTO, D. D.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. Identificação, classificação e caracterização transcricional das desidrinas de macieira. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, p. 620_623, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 129. | | SILVA, D. A. I. da; CZERMAINSKI, A. B. C.; REVERS, L. F.; VIEIRA, H. J.; MISZINSKI, J. Quantificação do frio hibernal e sua associação com a brotação em macieira (Malus pumila L. Borkh). In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 14. ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 10., 2016, Bento Gonçalves. Resumos...Bento Gonçalves, RS: Embrapa uva e Vinho, 2016. p. 33. (Embrapa Uva e Vinho. Documentos, 99)| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 130. | | ALENCAR; SILVA JUNIOR, O. B. da; TOGAWA, R. C.; COSTA, M.; REVERS, L. F.; PAPPAS JUNIOR, G. J. SNP discovery in apple cultivars using next generation sequencing. BMC Proceedings, v. 5, Suppl 7,p. P42, 2011. Edição dos resumos do IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration, 2011, Arraial d Ajuda, Bahia.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 131. | | DUBIELA, C. R.; FAJARDO, T. V. M.; SOUTO, E. R.; NICKEL, O.; EIRAS, M.; REVERS, L. F. Simultaneous detection of Brazilian isolates of grapevine viruses by TaqMan real-time RT-PCR. Tropical Plant Pathology, Viçosa, v. 38, n. 2, p. 158-165, mar./apr. 2013 Comunicação científica.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 132. | | GARIGHAN, J. de A.; PASQUALI, G.; REVERS, L. F.; PORTO, D. D.; SOUZA, D. A.; SANTOS, H. P. dos. Hormonal balance characterization of dormant buds in apple. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstracts. [S.l.]: International Society for Plant Molecular Biology, 2015. p. 683.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 133. | | FALAVIGNA, V. da S.; PORTO, D. D.; BUFFON, V.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. Perfil transcricional em gemas dormentes de duas cultivares de macieira com requerimento de frio contrastante. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 10.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 6., 2012, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2012. p. 13. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 134. | | CARDONE, J. M.; REVERS, L. F.; MACHADO, R. M.; BONATTO, D.; BRENDEL, M.; HENRIQUES, J. A. P. Psoralen-sensitive mutant pso9-1 of Saccharomyces cerevisiae contains a mutant allele of the DNA damage checkpoint gene MEC3. DNA Repair, Amsterdam, v. 5, n. 2, p. 163-171, 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 135. | | FALAVIGNA, V. da S.; MIOTTO, Y. E.; PORTO, D. D.; ANZANELLO, R.; FIALHO, F. B.; SANTOS, H. P. dos; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. Análise da expressão de genes codificadores de desidrinas durante a dormência de gemas em macieira. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 11.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 7., Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2013. p. 20. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 137. | | CATTANI, A. M.; SARTOR, T.; FALAVIGNA, V. da S.; PORTO, D. D.; SILVEIRA, C. P.; OLIVEIRA, P. R. D. de; REVERS, L. F. The control of bud break and flowering time in plants: contribution of epigenetic mechanisms and consequences in agriculture and breeding. In: MIROUZE, M.; BUCHER, E.; GALLUSCI, P. Advances in Botanical Research: Plant epigenetics coming of age ofr breeding applications. Elsevier: Academic Press, 2018. Cap. 9, p. 277-325.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 139. | | ALMEIDA, G. S. d'; BRBETON, M. C.; MARTINS, P. D.; FALAVIGNA, V. da S.; TURCHETTO-ZOLET, A. C.; REVERS, L. F.; PASQUALI, G. Characterization of eucalyptus grandis dof gene family of transcription factors. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: Sociedade Brasileira de Genética, 2013. p. 17. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| 140. | | ALMEIDA, G. S. d’; BRETON, M. C.; MARTINS, P. D.; FALAVIGNA, V. da S.; TURCHETTO-ZOLET, A. C.; REVERS, L. F.; PASQUALI, G. Characterization of Eucalyptus grandis' DOF gene family of transcription factors. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: Sociedade Brasileira de Genética, 2013. p. 17. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
| |
| Registros recuperados : 174 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
