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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
20/01/2005 |
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Data da última atualização: |
25/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MEDEIROS, R. B.; RESENDE, R. de O.; AVILA, A. C. de. |
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Afiliação: |
RICARDO B. MEDEIROS, UNIVERSIDADE DE BRASÍLIA; RENATO DE OLIVEIRA RESENDE, UNIVERSIDADE DE BRASÍLIA; ANTONIO CARLOS DE AVILA, CNPH. |
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Título: |
The plant virus tomato spotted wilt tospovirus activates the immune system of its main insect vector, Frankliniella occidentalis. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Journal of Virology, v. 78, n. 10, p. 4976-4982, 2004. |
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ISSN: |
1098-5514 |
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Idioma: |
Inglês |
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Conteúdo: |
Tospoviruses have the ability to infect plants and their insect vectors. Tomato spotted wilt virus (TSWV), the type species in the Tospovirus genus, infects its most important insect vector, Frankliniella occidentalis, the western flower thrips (WFT). However, no detrimental effects on the life cycle or cytopathological changes have been reported in the WFT after TSWV infection, and relatively few viral particles can be observed even several days after infection. We hypothesized that TSWV infection triggers an immune response in the WFT. Using subtractive cDNA libraries to probe WFT DNA macroarrays, we found that the WFT's immune system is activated by TSWV infection. The activated genes included (i) those encoding antimicrobial peptides, such as defensin and cecropin; (ii) genes involved in pathogen recognition, such as those encoding lectins; (iii) those encoding receptors that activate the innate immune response, such as Toll-3; and (iv) those encoding members of signal transduction pathways activated by Toll-like receptors, such as JNK kinase. Transcriptional upregulation of these genes after TSWV infection was confirmed by Northern analysis, and the kinetics of the immune response was measured over time. Several of the detected genes were activated at the same time that viral replication was first detected by reverse transcription-PCR. To our knowledge, this is the first report of the activation of an insect vector immune response by a plant virus. The results may lead to a better understanding of insects' immune responses against viruses and may help in the future development of novel control strategies against plant viruses, as well as human and animal viruses transmitted by insect vectors. MenosTospoviruses have the ability to infect plants and their insect vectors. Tomato spotted wilt virus (TSWV), the type species in the Tospovirus genus, infects its most important insect vector, Frankliniella occidentalis, the western flower thrips (WFT). However, no detrimental effects on the life cycle or cytopathological changes have been reported in the WFT after TSWV infection, and relatively few viral particles can be observed even several days after infection. We hypothesized that TSWV infection triggers an immune response in the WFT. Using subtractive cDNA libraries to probe WFT DNA macroarrays, we found that the WFT's immune system is activated by TSWV infection. The activated genes included (i) those encoding antimicrobial peptides, such as defensin and cecropin; (ii) genes involved in pathogen recognition, such as those encoding lectins; (iii) those encoding receptors that activate the innate immune response, such as Toll-3; and (iv) those encoding members of signal transduction pathways activated by Toll-like receptors, such as JNK kinase. Transcriptional upregulation of these genes after TSWV infection was confirmed by Northern analysis, and the kinetics of the immune response was measured over time. Several of the detected genes were activated at the same time that viral replication was first detected by reverse transcription-PCR. To our knowledge, this is the first report of the activation of an insect vector immune response by a plant virus. The results may lead ... Mostrar Tudo |
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Palavras-Chave: |
Interação vírus vetor; TSWV. |
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Thesagro: |
Inseto; Lycopersicon Esculentum; Praga; Tomate; Vetor; Vírus. |
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Thesaurus Nal: |
Frankliniella occidentalis; Tospovirus. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 02535naa a2200277 a 4500
001 1777605
005 2024-01-25
008 2004 bl uuuu u00u1 u #d
022 $a1098-5514
100 1 $aMEDEIROS, R. B.
245 $aThe plant virus tomato spotted wilt tospovirus activates the immune system of its main insect vector, Frankliniella occidentalis.$h[electronic resource]
260 $c2004
520 $aTospoviruses have the ability to infect plants and their insect vectors. Tomato spotted wilt virus (TSWV), the type species in the Tospovirus genus, infects its most important insect vector, Frankliniella occidentalis, the western flower thrips (WFT). However, no detrimental effects on the life cycle or cytopathological changes have been reported in the WFT after TSWV infection, and relatively few viral particles can be observed even several days after infection. We hypothesized that TSWV infection triggers an immune response in the WFT. Using subtractive cDNA libraries to probe WFT DNA macroarrays, we found that the WFT's immune system is activated by TSWV infection. The activated genes included (i) those encoding antimicrobial peptides, such as defensin and cecropin; (ii) genes involved in pathogen recognition, such as those encoding lectins; (iii) those encoding receptors that activate the innate immune response, such as Toll-3; and (iv) those encoding members of signal transduction pathways activated by Toll-like receptors, such as JNK kinase. Transcriptional upregulation of these genes after TSWV infection was confirmed by Northern analysis, and the kinetics of the immune response was measured over time. Several of the detected genes were activated at the same time that viral replication was first detected by reverse transcription-PCR. To our knowledge, this is the first report of the activation of an insect vector immune response by a plant virus. The results may lead to a better understanding of insects' immune responses against viruses and may help in the future development of novel control strategies against plant viruses, as well as human and animal viruses transmitted by insect vectors.
650 $aFrankliniella occidentalis
650 $aTospovirus
650 $aInseto
650 $aLycopersicon Esculentum
650 $aPraga
650 $aTomate
650 $aVetor
650 $aVírus
653 $aInteração vírus vetor
653 $aTSWV
700 1 $aRESENDE, R. de O.
700 1 $aAVILA, A. C. de
773 $tJournal of Virology$gv. 78, n. 10, p. 4976-4982, 2004.
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Embrapa Hortaliças (CNPH) |
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| Registros recuperados : 83 | |
| 22. |  | FAJARDO, T. V. M.; AVILA, A. C. de; BUSO, J. A.; RESENDE, R. de O. Identification of two potyviruses in mixed infection of garlic plants by molecular techniques. Fitopatologia Brasileira, Brasília, DF, v. 22, p. 334, ago. 1997. Resumo 584. Congresso Brasileiro de Fitopatologia, 30, 1997.| Biblioteca(s): Embrapa Hortaliças. |
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| 27. | | FAJARDO, T. V. M.; AVILA, A. C. de; BUSO, J. A.; RESENDE, R. de O. Molecular characterization of garlic common latent virus(GCLV), a carlavirus, in garlic viral complex. Fitopatologia Brasileira, Brasilia, v.22, p.334, 1997. Suplemento. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 28. | | FAJARDO, T. V. M.; AVILA, A. C. de; BUSO, J. A.; RESENDE, R. de O. Molecular characterization of garlic viral complex. Virus Reviews Research, Sao Paulo, v.2, n,1-2, p.191, nov. 1997. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 31. | | LOVATO, F. A.; BEZERRA, I. C.; RESENDE, R. de O.; FERREIRA, A. T.; TORRES, A. C. Transformacao genetica da alface, cultivar Veronica, via Agrobecterium tumefaciens. Revista Brasileira de Fisiologia Vegetal, Brasilia v.10, n.3, p.219-224, 1998.| Biblioteca(s): Embrapa Hortaliças. |
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| 32. | | AVILA, A. C. de; GIORDANO, B.; BEZERRA, C. I.; LOVATO, F. A.; RESENDE, R. de O. Strategies to control tospovirus on vegetable crops in Brazil. Virus Reviews and Research, Sao Paulo, v.2, n.1-2, p.134-135, nov. 1997. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 33. | | EIRAS, M.; MISSIAGGIA, A. A.; RESENDE, R. de O.; BEZERRA, I. C.; AVILA, A. C. de. Strategies for tospovirus direction by RT-PCR. Fitopatologia Brasileira, Brasília, DF, v. 22, p. 333, ago. 1997. Suplemento. Resumo 592. Congresso Brasileiro de Fitopatologia, 30., 1997.| Biblioteca(s): Embrapa Hortaliças. |
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| 34. | | SILVA, M. S.; MARTINS, C. R. F.; BEZERRA, M. I.; NAGATA, T.; ÁVILA, A. C.; RESENDE, R. de O. Analysis of conserved domais present in the movemet protein of tospoviruses. Virus Reviews & Research, Florianópolis, v. 5, n. 2, p. 193, 2000. Suplemento 1. Resumo. Trabalho apresentado no 11º National Meeting of Virology, São Lourenço, 2000.| Biblioteca(s): Embrapa Hortaliças. |
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| 36. | | AVILA, A. C. de; HAAN, P. de; KITAJIMA, E. W.; RESENDE, R. de O.; GOLDBACH, R. W.; PETERS, D. Characterization of a distinct isolate of tomato spotted wilt virus (TSWV) from impatiens in the Netherlands. Fitopatologia Brasileira, Brasilia, v.16, n.2, p.25, jun. 1991. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 37. | | POZZER, L.; BEZERRA, I. C.; KORMELINK, R.; PRINS, M.; PETERS, D.; RESENDE, R. de O.; AVILA, A. C. de. Characterization of a tospovirus isolate of iris yellow spot virus associated with a disease in onion fields in Brazil. Plant Diseases, v.83, n.4, p.345-350, Apr. 1999.| Biblioteca(s): Embrapa Hortaliças. |
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| 39. | | AVILA, A. C. de; RESENDE, R. de O.; BEZERRA, I. C.; POZZER, L.; NAGATA, T.; KITAJIMA, E. W. The potential use of Polymerase Chain Reaction on the detection of tospovirus species. Fitopatologia Brasileira, Brasilia, v.20, p.301, ago. 1995. Suplemento. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 40. | | TORRES, A. C.; FAJARDO, T. V. M.; DUSI, A. N.; RESENDE, R. de O.; BUSO, J. A. Shoot tip culture and thermotherapy of recovering virus-free plants of garlic. Horticultura Brasileira, Brasilia, v.18, n.3, p.192-195, nov. 2000.| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 83 | |
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