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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
15/06/2004 |
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Data da última atualização: |
25/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MEDEIROS, R. B.; RESENDE, R. de O.; AVILA, A. C. de. |
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Afiliação: |
RICARDO B. MEDEIROS, UNIVERSIDADE DE BRASÍLIA; RENATO DE O. RESENDE, UNIVERSIDADE DE BRASÍLIA; ANTONIO CARLOS DE AVILA, CNPH. |
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Título: |
The plant virus tomato spotted wilt tospovirus activates the immune system of its main insect vector, Frakliniella occidentalis. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Journal of Virology, v. 78, n. 10, p. 4976-4982, May 2004. |
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ISSN: |
1098-5514 |
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DOI: |
10.1128/JVI.78.10.4976–4982.2004 |
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Idioma: |
Inglês |
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Conteúdo: |
Tospoviruses have the ability to infect plants and their insect vectors. Tomato spotted wilt virus (TSWV), the type species in the Tospovirus genus, infects its most important insect vector, Frankliniella occidentalis, the western flower thrips (WFT). However, no detrimental effects on the life cycle or cytopathological changes have been reported in the WFT after TSWV infection, and relatively few viral particles can be observed even several days after infection. We hypothesized that TSWV infection triggers an immune response in the WFT. Using subtractive cDNA libraries to probe WFT DNA macroarrays, we found that the WFT’s immune system is activated by TSWV infection. The activated genes included (i) those encoding antimicrobial peptides, such as defensin and cecropin; (ii) genes involved in pathogen recognition, such as those encoding lectins; (iii) those encoding receptors that activate the innate immune response, such as Toll-3; and (iv) those encoding members of signal transduction pathways activated by Toll-like receptors, such as JNK kinase. Transcriptional upregulation of these genes after TSWV infection was confirmed by Northern analysis, and the kinetics of the immune response was measured over time. Several of the detected genes were activated at the same time that viral replication was first detected by reverse transcription-PCR. To our knowledge, this is the first report of the activation of an insect vector immune response by a plant virus. The results may lead to a better understanding of insects’ immune responses against viruses and may help in the future development of novel control strategies against plant viruses, as well as human and animal viruses transmitted by insect vectors. MenosTospoviruses have the ability to infect plants and their insect vectors. Tomato spotted wilt virus (TSWV), the type species in the Tospovirus genus, infects its most important insect vector, Frankliniella occidentalis, the western flower thrips (WFT). However, no detrimental effects on the life cycle or cytopathological changes have been reported in the WFT after TSWV infection, and relatively few viral particles can be observed even several days after infection. We hypothesized that TSWV infection triggers an immune response in the WFT. Using subtractive cDNA libraries to probe WFT DNA macroarrays, we found that the WFT’s immune system is activated by TSWV infection. The activated genes included (i) those encoding antimicrobial peptides, such as defensin and cecropin; (ii) genes involved in pathogen recognition, such as those encoding lectins; (iii) those encoding receptors that activate the innate immune response, such as Toll-3; and (iv) those encoding members of signal transduction pathways activated by Toll-like receptors, such as JNK kinase. Transcriptional upregulation of these genes after TSWV infection was confirmed by Northern analysis, and the kinetics of the immune response was measured over time. Several of the detected genes were activated at the same time that viral replication was first detected by reverse transcription-PCR. To our knowledge, this is the first report of the activation of an insect vector immune response by a plant virus. The results may lead ... Mostrar Tudo |
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Palavras-Chave: |
TSWV. |
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Thesagro: |
Inseto; Lycopersicon Esculentum; Praga; Tomate; Vetor; Vírus. |
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Thesaurus Nal: |
Frankliniella occidentalis. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 02530naa a2200265 a 4500
001 1776095
005 2024-01-25
008 2004 bl uuuu u00u1 u #d
022 $a1098-5514
024 7 $a10.1128/JVI.78.10.4976–4982.2004$2DOI
100 1 $aMEDEIROS, R. B.
245 $aThe plant virus tomato spotted wilt tospovirus activates the immune system of its main insect vector, Frakliniella occidentalis.$h[electronic resource]
260 $c2004
520 $aTospoviruses have the ability to infect plants and their insect vectors. Tomato spotted wilt virus (TSWV), the type species in the Tospovirus genus, infects its most important insect vector, Frankliniella occidentalis, the western flower thrips (WFT). However, no detrimental effects on the life cycle or cytopathological changes have been reported in the WFT after TSWV infection, and relatively few viral particles can be observed even several days after infection. We hypothesized that TSWV infection triggers an immune response in the WFT. Using subtractive cDNA libraries to probe WFT DNA macroarrays, we found that the WFT’s immune system is activated by TSWV infection. The activated genes included (i) those encoding antimicrobial peptides, such as defensin and cecropin; (ii) genes involved in pathogen recognition, such as those encoding lectins; (iii) those encoding receptors that activate the innate immune response, such as Toll-3; and (iv) those encoding members of signal transduction pathways activated by Toll-like receptors, such as JNK kinase. Transcriptional upregulation of these genes after TSWV infection was confirmed by Northern analysis, and the kinetics of the immune response was measured over time. Several of the detected genes were activated at the same time that viral replication was first detected by reverse transcription-PCR. To our knowledge, this is the first report of the activation of an insect vector immune response by a plant virus. The results may lead to a better understanding of insects’ immune responses against viruses and may help in the future development of novel control strategies against plant viruses, as well as human and animal viruses transmitted by insect vectors.
650 $aFrankliniella occidentalis
650 $aInseto
650 $aLycopersicon Esculentum
650 $aPraga
650 $aTomate
650 $aVetor
650 $aVírus
653 $aTSWV
700 1 $aRESENDE, R. de O.
700 1 $aAVILA, A. C. de
773 $tJournal of Virology$gv. 78, n. 10, p. 4976-4982, May 2004.
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Embrapa Hortaliças (CNPH) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
20/10/2014 |
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Data da última atualização: |
11/01/2016 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
GOMES, A. da S.; GONÇALVES, F. M.; SENTO-SÉ, G. V. T.; FERREIRA, T. D.; COSTA, N. D.; YURI, J. E.; RESENDE, G. M. de. |
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Afiliação: |
ADRIANO DA SILVA GOMES; FERNANDA MATIAS GONÇALVES; GEORGGE V. T. SENTO-SÉ; TAINÁ DOURADO FERREIRA; NIVALDO DUARTE COSTA, CPATSA; JONY EISHI YURI, CPATSA; GERALDO MILANEZ DE RESENDE, CPATSA. |
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Título: |
Competição de cultivares de tomate tipo salada No Submédio do Vale do São Francisco nas condições de verão. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Horticultura Brasileira, Brasília, DF, v. 31, p. S0771-S0776, 2014. |
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Idioma: |
Português |
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Notas: |
Suplemento. Edição dos Anais do 53 Congresso Brasileiro de Olericultura, jul. 2014. |
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Conteúdo: |
O presente trabalho teve por objetivo avaliar o comportamento produtivo de cultivares de tomate do tipo salada, com hábito de crescimento determinado, nas condições de verão do Submédio do Vale do São Francisco. |
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Palavras-Chave: |
Cultivo; Tomate para salada; Tomato. |
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Thesagro: |
Tomate. |
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Thesaurus NAL: |
Solanum lycopersicum. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/110192/1/Jony-3.pdf
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Marc: |
LEADER 01063nam a2200253 a 4500
001 1997715
005 2016-01-11
008 2014 bl uuuu u00u1 u #d
100 1 $aGOMES, A. da S.
245 $aCompetição de cultivares de tomate tipo salada No Submédio do Vale do São Francisco nas condições de verão.$h[electronic resource]
260 $aHorticultura Brasileira, Brasília, DF, v. 31, p. S0771-S0776$c2014
500 $aSuplemento. Edição dos Anais do 53 Congresso Brasileiro de Olericultura, jul. 2014.
520 $aO presente trabalho teve por objetivo avaliar o comportamento produtivo de cultivares de tomate do tipo salada, com hábito de crescimento determinado, nas condições de verão do Submédio do Vale do São Francisco.
650 $aSolanum lycopersicum
650 $aTomate
653 $aCultivo
653 $aTomate para salada
653 $aTomato
700 1 $aGONÇALVES, F. M.
700 1 $aSENTO-SÉ, G. V. T.
700 1 $aFERREIRA, T. D.
700 1 $aCOSTA, N. D.
700 1 $aYURI, J. E.
700 1 $aRESENDE, G. M. de
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