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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
31/03/1993 |
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Data da última atualização: |
05/03/2024 |
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Autoria: |
KITAJIMA, E. W.; RESENDE, R. de O.; AVILA, A. C. de; GOLDBACH, R. W.; PETERS, D. |
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Afiliação: |
ANTONIO CARLOS DE AVILA, CNPH. |
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Título: |
Immuno-electron microscopical detection of tomato spotted wilt virus and its nucleocapsids in crude plant extracts. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Journal of Virological Methods, v. 38, n. 3, p. 313-322, Aug./Sept. 1992. |
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DOI: |
https://doi.org/10.1016/0166-0934(92)90076-P |
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Idioma: |
Inglês |
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Conteúdo: |
Tomato spotted wilt virus (TSWV) particles were identified in thin sections and in crude extracts of leaves from plants infected with different TSWV isolates, using gold labelled protein A and antibodies prepared against purified virus particles or against nucleocapsid preparations. In addition, both in thin sections and in dip preparations aggregates were detected using either goldlabelled antiserum. These aggregates were not detected using antisera against envelope proteins or against the non-structural protein NSs. They were the only detectably labelled material in preparations made from plants infected by morphologically defective isolates of TSWV, i.e. isolates that did not produce enveloped particles. The aggregates were discerned in dip preparations as cloudy amorphous structures having a cottonwool boll-like appearance. These ‘cotton bolls’ were interpreted as being the in vitro structures of the nucleocapsid aggregates occurring in plant cells infected by isolates maintained by mechanical inoculation and are the only structures in cells infected by morphologically defective isolates (Ie, 1982; Kitajima et al., 1992). The detection of the ‘cotton boll-like’ structures in crude extracts provides an adequate and rapid method to establish the presence of nucleocapsid aggregates in cells of infected plants, irrespective of whether they are infected by morphologically defective isolates or not. |
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Palavras-Chave: |
Deteccao; Immunogold; Imunoflorescencia; Labelling; TSWV; Vira-Cabeca. |
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Thesaurus Nal: |
detection; Tomato spotted wilt virus. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02285naa a2200277 a 4500
001 1749564
005 2024-03-05
008 1992 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/0166-0934(92)90076-P$2DOI
100 1 $aKITAJIMA, E. W.
245 $aImmuno-electron microscopical detection of tomato spotted wilt virus and its nucleocapsids in crude plant extracts.$h[electronic resource]
260 $c1992
520 $aTomato spotted wilt virus (TSWV) particles were identified in thin sections and in crude extracts of leaves from plants infected with different TSWV isolates, using gold labelled protein A and antibodies prepared against purified virus particles or against nucleocapsid preparations. In addition, both in thin sections and in dip preparations aggregates were detected using either goldlabelled antiserum. These aggregates were not detected using antisera against envelope proteins or against the non-structural protein NSs. They were the only detectably labelled material in preparations made from plants infected by morphologically defective isolates of TSWV, i.e. isolates that did not produce enveloped particles. The aggregates were discerned in dip preparations as cloudy amorphous structures having a cottonwool boll-like appearance. These ‘cotton bolls’ were interpreted as being the in vitro structures of the nucleocapsid aggregates occurring in plant cells infected by isolates maintained by mechanical inoculation and are the only structures in cells infected by morphologically defective isolates (Ie, 1982; Kitajima et al., 1992). The detection of the ‘cotton boll-like’ structures in crude extracts provides an adequate and rapid method to establish the presence of nucleocapsid aggregates in cells of infected plants, irrespective of whether they are infected by morphologically defective isolates or not.
650 $adetection
650 $aTomato spotted wilt virus
653 $aDeteccao
653 $aImmunogold
653 $aImunoflorescencia
653 $aLabelling
653 $aTSWV
653 $aVira-Cabeca
700 1 $aRESENDE, R. de O.
700 1 $aAVILA, A. C. de
700 1 $aGOLDBACH, R. W.
700 1 $aPETERS, D.
773 $tJournal of Virological Methods$gv. 38, n. 3, p. 313-322, Aug./Sept. 1992.
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Embrapa Hortaliças (CNPH) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
04/10/2011 |
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Data da última atualização: |
13/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
RAKOCEVIC, M.; COSTES, E.; ASSAD, E. D. |
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Afiliação: |
IAPAR; E. COSTES, INRA; EDUARDO DELGADO ASSAD, CNPTIA. |
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Título: |
Structural and physiological sexual dimorphism estimated from three-dimensional virtual trees of yerba-mate (Ilex paraguariensis) is modified by cultivation environment. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Annals of Applied Biology, Cambridge, v. 159, n. 2, p. 178-191, 2011. |
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DOI: |
10.1111/j.1744-7348.2011.00484.x |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract - Yerba-mate is a subtropical, evergreen, dioecious, South American tree. Sexual dimorphism in photosynthesis, leaf allometry and foliage distribution was hypothesised. Virtual trees (constructed in VPlants software from detailed measurements of plant morphogenesis) of the two genders were compared considering two contrasted cultivation environments and three developmental stages. The total crown volume, leaf area per plant (LA), leaf area index (LAI) and leaf area density (LAD) were calculated. The light interception and photosynthesis were computed from mock-ups in VegeSTAR. Structural sexual dimorphism concerned general plant form, internode length, leaf allometry, leaf surface, pattern of leaf area distribution and LAD. Cultivation environment and developmental stage acted strongly on sex expression of all observed structural parameters and physiological stages. Sexual differentiation in LA and light interception was related to leaves positioned in the lowest layers (150 cm above ground), whereas sexual specialisation in leaf and plant photosynthesis was related to early vegetative and reproductive stages. Several sexual responses strongly depended on the environment, especially light conditions, with opposite effects observed on female and male plants whether they were cultivated in monoculture or in forest understorey, under highlight condition or low-light condition, respectively. Optimised foliage structure and physiology in females may compensate for greater reproductive costs in early developmental stages, but females and males equalise in photosynthetic efficiency after 2-year regrowth. MenosAbstract - Yerba-mate is a subtropical, evergreen, dioecious, South American tree. Sexual dimorphism in photosynthesis, leaf allometry and foliage distribution was hypothesised. Virtual trees (constructed in VPlants software from detailed measurements of plant morphogenesis) of the two genders were compared considering two contrasted cultivation environments and three developmental stages. The total crown volume, leaf area per plant (LA), leaf area index (LAI) and leaf area density (LAD) were calculated. The light interception and photosynthesis were computed from mock-ups in VegeSTAR. Structural sexual dimorphism concerned general plant form, internode length, leaf allometry, leaf surface, pattern of leaf area distribution and LAD. Cultivation environment and developmental stage acted strongly on sex expression of all observed structural parameters and physiological stages. Sexual differentiation in LA and light interception was related to leaves positioned in the lowest layers (150 cm above ground), whereas sexual specialisation in leaf and plant photosynthesis was related to early vegetative and reproductive stages. Several sexual responses strongly depended on the environment, especially light conditions, with opposite effects observed on female and male plants whether they were cultivated in monoculture or in forest understorey, under highlight condition or low-light condition, respectively. Optimised foliage structure and physiology in females may compensate for greate... Mostrar Tudo |
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Palavras-Chave: |
Árvores virtuais; Dimorfismo sexual; Erva-mate. |
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Thesagro: |
Área Foliar; Fotossíntese; Ilex Paraguariensis. |
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Thesaurus NAL: |
Leaf area; Photosynthesis; Sexual dimorphism. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02508naa a2200265 a 4500
001 1902281
005 2020-01-13
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1111/j.1744-7348.2011.00484.x$2DOI
100 1 $aRAKOCEVIC, M.
245 $aStructural and physiological sexual dimorphism estimated from three-dimensional virtual trees of yerba-mate (Ilex paraguariensis) is modified by cultivation environment.$h[electronic resource]
260 $c2011
520 $aAbstract - Yerba-mate is a subtropical, evergreen, dioecious, South American tree. Sexual dimorphism in photosynthesis, leaf allometry and foliage distribution was hypothesised. Virtual trees (constructed in VPlants software from detailed measurements of plant morphogenesis) of the two genders were compared considering two contrasted cultivation environments and three developmental stages. The total crown volume, leaf area per plant (LA), leaf area index (LAI) and leaf area density (LAD) were calculated. The light interception and photosynthesis were computed from mock-ups in VegeSTAR. Structural sexual dimorphism concerned general plant form, internode length, leaf allometry, leaf surface, pattern of leaf area distribution and LAD. Cultivation environment and developmental stage acted strongly on sex expression of all observed structural parameters and physiological stages. Sexual differentiation in LA and light interception was related to leaves positioned in the lowest layers (150 cm above ground), whereas sexual specialisation in leaf and plant photosynthesis was related to early vegetative and reproductive stages. Several sexual responses strongly depended on the environment, especially light conditions, with opposite effects observed on female and male plants whether they were cultivated in monoculture or in forest understorey, under highlight condition or low-light condition, respectively. Optimised foliage structure and physiology in females may compensate for greater reproductive costs in early developmental stages, but females and males equalise in photosynthetic efficiency after 2-year regrowth.
650 $aLeaf area
650 $aPhotosynthesis
650 $aSexual dimorphism
650 $aÁrea Foliar
650 $aFotossíntese
650 $aIlex Paraguariensis
653 $aÁrvores virtuais
653 $aDimorfismo sexual
653 $aErva-mate
700 1 $aCOSTES, E.
700 1 $aASSAD, E. D.
773 $tAnnals of Applied Biology, Cambridge$gv. 159, n. 2, p. 178-191, 2011.
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Embrapa Agricultura Digital (CNPTIA) |
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