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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
14/09/1995 |
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Data da última atualização: |
14/09/1995 |
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Autoria: |
ROBERTS, H. M.; REISENAUER, H. M. |
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Afiliação: |
Department of Land, Air and Water Resources. |
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Título: |
Determination of ammonium, amides, and soluble carboxylates in plants tissues. |
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Ano de publicação: |
1980 |
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Fonte/Imprenta: |
Journal of Plant Nutrition, v.2, n4, p.395-405, 1980. |
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Idioma: |
Inglês |
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Conteúdo: |
Methods for extraction and determination of ammonium, amides and soluble carboxylates in plant tissues are compared and discussed. The procedure recommended involves extraction of finely ground plant tissues with 0.2 M formic acid, determination of ammonium and amides in the extract by overnight alkaline hydrolysis and distillation in Conway microdiffusion dishes, measurement of extracted ammonium using Carlson's rapid diffusion-conductivity instrument, and estimation of soluble organic acids by titration after separation and purification with ion exhange resins. The method described is reasonably rapid. The final solution of organic acids is suitable for direct chromatographic analysis. |
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Palavras-Chave: |
Extraction methods; Organic acids. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01151naa a2200157 a 4500
001 1647082
005 1995-09-14
008 1980 bl --- 0-- u #d
100 1 $aROBERTS, H. M.
245 $aDetermination of ammonium, amides, and soluble carboxylates in plants tissues.
260 $c1980
520 $aMethods for extraction and determination of ammonium, amides and soluble carboxylates in plant tissues are compared and discussed. The procedure recommended involves extraction of finely ground plant tissues with 0.2 M formic acid, determination of ammonium and amides in the extract by overnight alkaline hydrolysis and distillation in Conway microdiffusion dishes, measurement of extracted ammonium using Carlson's rapid diffusion-conductivity instrument, and estimation of soluble organic acids by titration after separation and purification with ion exhange resins. The method described is reasonably rapid. The final solution of organic acids is suitable for direct chromatographic analysis.
653 $aExtraction methods
653 $aOrganic acids
700 1 $aREISENAUER, H. M.
773 $tJournal of Plant Nutrition$gv.2, n4, p.395-405, 1980.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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