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Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
21/06/2001 |
Data da última atualização: |
21/06/2001 |
Autoria: |
JAMES, E. K.; OLIVARES, F. L.; OLIVEIRA, A. L. M. de; REIS JÚNIOR, F. B. dos; SILVA, L. G. da; REIS, V. M. |
Título: |
Further observations on the interaction between sugar can and Gluconacetobacter diazotrophicus under laboratory and greenhouse conditions. |
Ano de publicação: |
2001 |
Fonte/Imprenta: |
Journal of Experimental Botany, London, v. 52, n. 357, p. 747-760, apr. 2001. |
Idioma: |
Inglês |
Conteúdo: |
Sugar cane (Saccharum spp.) variety SP 70-1143 was inoculated with Gluconacetobacter diazotrophicus strain PAL5 (ATCC 49037) in two experiments. In experiment 1 the bacteria were inoculated into a modified, low sucrose MS medium within which micropropagated plantlets were rooted. After 10 d there was extensive anatomical evidence of endophytic colonization by G. diazotrophicus, particularly in lower stems, where high numbers of bacteria were visible within some of the xylem vessels. The identity of the bacteria was confirmed by immunogold label- ling with an antibody raised against G. diazotrophicus. On the lower stems there were breaks caused by the separation of the plantlets into individuais, and at these "wounds' bacteria were seen colonizing the xylem and intercellular spaces. Bacteria were also occasionally seen entering leaves via damaged stomata, and subsequently colonizing sub-stomatal cavities and intercellular spaces. A localized host defence response in the form of fibrillar material surrounding the bacteria was associated with both the stem and leaf invasion. In experiment 2, stems of 5-week-old greenhouse-grown plants were inoculated by injection with a suspension of G. diazotrophicus containing 108 bacteria ml-1. No hypersensitive response (HR) was observed, and no symptoms were visible on the leaves and stems for the duration of the experiment (7 d). Close to the point of inoculation, G. diazotrophicus cells were observed within the protoxylem and the xylem parenchyma, where they were surrounded by fibrillar material that stained light-green with toluidine blue. In leaf samples taken up to 4 cm from the inoculation points, G. diazotrophicus cells were mainly found within the metaxylem, where they were surrounded by a light green-staining material. The bacteria were growing in relatively low numbers adjacent to the xylem cell walls, and they were separated from the host-derived material by electron-transparent 'haloes' that contained material that reacted with the G. diazotrophicus antibody. MenosSugar cane (Saccharum spp.) variety SP 70-1143 was inoculated with Gluconacetobacter diazotrophicus strain PAL5 (ATCC 49037) in two experiments. In experiment 1 the bacteria were inoculated into a modified, low sucrose MS medium within which micropropagated plantlets were rooted. After 10 d there was extensive anatomical evidence of endophytic colonization by G. diazotrophicus, particularly in lower stems, where high numbers of bacteria were visible within some of the xylem vessels. The identity of the bacteria was confirmed by immunogold label- ling with an antibody raised against G. diazotrophicus. On the lower stems there were breaks caused by the separation of the plantlets into individuais, and at these "wounds' bacteria were seen colonizing the xylem and intercellular spaces. Bacteria were also occasionally seen entering leaves via damaged stomata, and subsequently colonizing sub-stomatal cavities and intercellular spaces. A localized host defence response in the form of fibrillar material surrounding the bacteria was associated with both the stem and leaf invasion. In experiment 2, stems of 5-week-old greenhouse-grown plants were inoculated by injection with a suspension of G. diazotrophicus containing 108 bacteria ml-1. No hypersensitive response (HR) was observed, and no symptoms were visible on the leaves and stems for the duration of the experiment (7 d). Close to the point of inoculation, G. diazotrophicus cells were observed within the protoxylem and the xylem p... Mostrar Tudo |
Palavras-Chave: |
Bactéria endofítica; Biological nitrogen fixation; BNF; Cana-de-açúcar; Casa de vegetação; Endophytic bacteria; FBN; Fixação bológica de nitrogênio; Greenhouse; Saccharum spp. |
Thesaurus Nal: |
Gluconacetobacter diazotrophicus; sugarcane. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03060naa a2200325 a 4500 001 1598609 005 2001-06-21 008 2001 bl --- 0-- u #d 100 1 $aJAMES, E. K. 245 $aFurther observations on the interaction between sugar can and Gluconacetobacter diazotrophicus under laboratory and greenhouse conditions. 260 $c2001 520 $aSugar cane (Saccharum spp.) variety SP 70-1143 was inoculated with Gluconacetobacter diazotrophicus strain PAL5 (ATCC 49037) in two experiments. In experiment 1 the bacteria were inoculated into a modified, low sucrose MS medium within which micropropagated plantlets were rooted. After 10 d there was extensive anatomical evidence of endophytic colonization by G. diazotrophicus, particularly in lower stems, where high numbers of bacteria were visible within some of the xylem vessels. The identity of the bacteria was confirmed by immunogold label- ling with an antibody raised against G. diazotrophicus. On the lower stems there were breaks caused by the separation of the plantlets into individuais, and at these "wounds' bacteria were seen colonizing the xylem and intercellular spaces. Bacteria were also occasionally seen entering leaves via damaged stomata, and subsequently colonizing sub-stomatal cavities and intercellular spaces. A localized host defence response in the form of fibrillar material surrounding the bacteria was associated with both the stem and leaf invasion. In experiment 2, stems of 5-week-old greenhouse-grown plants were inoculated by injection with a suspension of G. diazotrophicus containing 108 bacteria ml-1. No hypersensitive response (HR) was observed, and no symptoms were visible on the leaves and stems for the duration of the experiment (7 d). Close to the point of inoculation, G. diazotrophicus cells were observed within the protoxylem and the xylem parenchyma, where they were surrounded by fibrillar material that stained light-green with toluidine blue. In leaf samples taken up to 4 cm from the inoculation points, G. diazotrophicus cells were mainly found within the metaxylem, where they were surrounded by a light green-staining material. The bacteria were growing in relatively low numbers adjacent to the xylem cell walls, and they were separated from the host-derived material by electron-transparent 'haloes' that contained material that reacted with the G. diazotrophicus antibody. 650 $aGluconacetobacter diazotrophicus 650 $asugarcane 653 $aBactéria endofítica 653 $aBiological nitrogen fixation 653 $aBNF 653 $aCana-de-açúcar 653 $aCasa de vegetação 653 $aEndophytic bacteria 653 $aFBN 653 $aFixação bológica de nitrogênio 653 $aGreenhouse 653 $aSaccharum spp 700 1 $aOLIVARES, F. L. 700 1 $aOLIVEIRA, A. L. M. de 700 1 $aREIS JÚNIOR, F. B. dos 700 1 $aSILVA, L. G. da 700 1 $aREIS, V. M. 773 $tJournal of Experimental Botany, London$gv. 52, n. 357, p. 747-760, apr. 2001.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro Completo
Biblioteca(s): |
Embrapa Amapá. |
Data corrente: |
02/12/2008 |
Data da última atualização: |
13/10/2022 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SOARES, A. L. P.; GUEDES, M. C. |
Afiliação: |
ANA LAURA PINTO SOARES, UNIFAP; MARCELINO CARNEIRO GUEDES, CPAF-AP. |
Título: |
Aspectos da biologia do solo em áreas preparadas para plantio com e sem uso do fogo. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA UNIFAP, 4.; MOSTRA DE TCC, 3.; JORNADA DE INICIAÇÃO CIENTÍFICA DA SETEC, 4., 2008, Macapá. Livro de resumos... Macapá: SETEC: UNIFAP, 2008. |
Páginas: |
p. 107. |
Idioma: |
Português |
Thesagro: |
Capoeira; Queimada. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/124857/1/CPSF-AP-2008-ASPECTOS-BIOLOGIA-SOLO.pdf
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Marc: |
LEADER 00566nam a2200145 a 4500 001 1353048 005 2022-10-13 008 2008 bl uuuu u00u1 u #d 100 1 $aSOARES, A. L. P. 245 $aAspectos da biologia do solo em áreas preparadas para plantio com e sem uso do fogo. 260 $aIn: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA UNIFAP, 4.; MOSTRA DE TCC, 3.; JORNADA DE INICIAÇÃO CIENTÍFICA DA SETEC, 4., 2008, Macapá. Livro de resumos... Macapá: SETEC: UNIFAP$c2008 300 $ap. 107. 650 $aCapoeira 650 $aQueimada 700 1 $aGUEDES, M. C.
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Embrapa Amapá (CPAF-AP) |
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