Data/hora: 24/06/2024 22:20:41

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 27/12/2000
Data da última atualização: | 27/12/2000
Autoria: | REIS, V. M.; REIS JÚNIOR, F. B. dos; SALLES, J. F.; SCHLOTER, M.
Título: | Characterisation of different polyclonal antisera to quantify Herbaspirillum spp. in elephant grass (Pennisetum purpureum Schun.).
Ano de publicação: | 2000
Fonte/Imprenta: | Symbiosis, Rehovot, v. 29, p. 139-150, 2000.
Idioma: | Inglês
Conteúdo: | Immunological techniques can be applied to detect micro-organisms in different ecosystems. To follow the fate of Herbaspirillum seropedicae strain Z67 and H . rubrisubalbicans strain M4 in the rhizosphere of elephant grass, Pennisetum purpureum Schun. var. Capim Cana D'Africa, polyclonal antisera were raised against both strains. Both antisera were purified with protein-A, followed by a primary characterization for cross-reactivity. To reduce cross-reactivity for both sera, affinity purification was used for improvement. After that, both sera could be considered as species specific. The detection limit was 105 cells ml-l for the anti H. rubrisubalbicans serum and 1Q6 cells ml-l for the anti H. seropedicae serum. It was shown that two months after inoculation, both strains could be detected in the rhizoplane of elephant grass in high densities. The estimated numbers by ELISA were 107 cells g-1 fresh weight.
Palavras-Chave: | Bactéira diazotrófica; Diazotrophic bacteria; Elephant grass.
Thesagro: | Capim Elefante; Gramínea Forrageira; Pennisetum Purpureum.
Thesaurus Nal: | forage grasses; Herbaspirillum.
Categoria do assunto: | --
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (899-1) - UPC - SP - -- -  -  - 0022524 - DIS