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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
02/12/2020 |
Data da última atualização: |
18/12/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RECHENMACHER, C.; WIEBKE-STROHM, B.; OLIVEIRA-BUSATTO, L. A. de; WEBER, R. L. M.; CORSO, M. C. M.; LOPES-CAITAR, V. S.; SILVA, S. M. H; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C.; CARLINI, C. R.; BODANESE-ZANETTINI, M. H. |
Afiliação: |
Ciliana Rechenmacher, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS.; Beatriz Wiebke-Strohma, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS.; Luisa Abruzzi de Oliveira-Busatto, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS; Ricardo Luis Mayer Weber, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS.; Mariana Cristina Moraes Corso, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS; Valéria Stefania Lopes-Caitar, Department of Plant Sciences, University of Tennessee, Knoxville, TN, USA.; Suellen Mika Hishinuma Silva; WALDIR PEREIRA DIAS, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARA, CNPSO; Celia Regina Carlini, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS; Maria Helena Bodanese-Zanettini, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS. |
Título: |
Endogenous soybean peptide overexpression: an alternative to protect plants against root-knot nematodes |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Biotechnology Research & Innovation, v. 3, p. 10-18, 2020. |
DOI: |
10.1016/j.biori.2019.12.002. |
Idioma: |
Inglês |
Palavras-Chave: |
Agrobacterium rhizogenes; Resistência a nematóide; Soyuretox; Transformação genética. |
Thesagro: |
Glycine Max; Nematóide; Resistência; Soja. |
Thesaurus Nal: |
Genetic transformation. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 01092naa a2200349 a 4500 001 2127450 005 2020-12-18 008 2020 bl uuuu u00u1 u #d 024 7 $a10.1016/j.biori.2019.12.002.$2DOI 100 1 $aRECHENMACHER, C. 245 $aEndogenous soybean peptide overexpression$ban alternative to protect plants against root-knot nematodes$h[electronic resource] 260 $c2020 650 $aGenetic transformation 650 $aGlycine Max 650 $aNematóide 650 $aResistência 650 $aSoja 653 $aAgrobacterium rhizogenes 653 $aResistência a nematóide 653 $aSoyuretox 653 $aTransformação genética 700 1 $aWIEBKE-STROHM, B. 700 1 $aOLIVEIRA-BUSATTO, L. A. de 700 1 $aWEBER, R. L. M. 700 1 $aCORSO, M. C. M. 700 1 $aLOPES-CAITAR, V. S. 700 1 $aSILVA, S. M. H 700 1 $aDIAS, W. P. 700 1 $aMARCELINO-GUIMARÃES, F. C. 700 1 $aCARLINI, C. R. 700 1 $aBODANESE-ZANETTINI, M. H. 773 $tBiotechnology Research & Innovation$gv. 3, p. 10-18, 2020.
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Embrapa Soja (CNPSO) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Caprinos e Ovinos. |
Data corrente: |
27/09/2019 |
Data da última atualização: |
07/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
AZEVEDO, D. A. A. de; MONTEIRO, J. P.; PINHEIRO, R. R.; MUDADU, M. de A.; ANDRIOLI, A.; ARAÚJO, J. F.; SOUSA, A. L. M. de; SIDER, L. H.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. |
Afiliação: |
DALVA ALANA ARAGÃO DE AZEVEDO, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; JOMAR PATRICIO MONTEIRO, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; MAURICIO DE ALVARENGA MUDADU, CNPTIA; ALICE ANDRIOLI, CNPC; JUSCILÂNIA FURTADO ARAÚJO, Northeast Biotechnology Network, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; ANA LÍDIA MADEIRA DE SOUSA, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; LUCIA HELENA SIDER, CNPC; RENATO MESQUITA PEIXOTO, Universidade Federal do Acre (UFAC) - Rio Branco, AC, Brazil; MARIA FÁTIMA DA SILVA TEIXEIRA, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil. |
Título: |
Molecular characterization of circulating strains of small ruminant lentiviruses in Brazil based on complete gag and pol genes. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Small Ruminant Research, v. 177, p. 160-166, 2019. |
DOI: |
https://doi.org/10.1016/j.smallrumres.2019.06.011 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Small ruminant lentiviruses (SRLV) are widely distributed in the world and cause productive and economic losses. These pathogens have high genetic variability, which hinders diagnosis by molecular methods. Molecular characterization studies are good strategies for elaborating programs aimed to control or eradicate diseases. Hence, this study aimed to sequence, characterize and compare strains of SRLV, which were isolated from naturally infected goats from the states of Ceará, Rio Grande do Norte and Minas Gerais, Brazil, based on total nucleotide sequences of gag and pol genes. SRLV isolates from the microbiological collection of Sheep and Goat of Embrapa were cultured in goat synovial membrane. Supernatants containing cells were stored and viral particles were purified with sucrose density gradient centrifugation for RNA extraction. Then, sequencing in HiSeq 2500 platform (Illumina) was performed to obtain the total genome of the SRLV strains. The software MEGA version 7.0 was used for sequence editing and phylogeny analysis. A total of 17 isolates were multiplied in cellular culture and six were viable, which were two from Minas Gerais (MG), one from Rio Grande do Norte (RN) and three from Ceará (CE). Two sequences were obtained from the sequencing by synthesis, one from MG (BRMG CNPC) and one from RN (BRRN CNPC). From the total SRLV genome (9,200 bases), sample BRMG CNPC generated a consensus of 6,378 bases, approximately 69,34% of the viral genome and sample BRRN CNPC generated a consensus of 5,351 bases, which comprised 58.16% of the total genome. Samples in this study demonstrated closer phylogenetic relation with genotype B (B1 group). The remaining strains sequenced in Brazil are also present in the same group with the exception of three samples from genotype A. Immunodominant regions of gag gene and motifs of pol gene were similar to the standard CAEV Cork strain. For the first time, the complete gag and pol genes were sequenced in Brazil from the strain BRMG CNPC isolated in Minas Gerais state. In addition, 2490 bases from the total of 3316 bases were sequenced from the pol gene of the BRRN CNPC sample from Rio Grande do Norte. MenosAbstract: Small ruminant lentiviruses (SRLV) are widely distributed in the world and cause productive and economic losses. These pathogens have high genetic variability, which hinders diagnosis by molecular methods. Molecular characterization studies are good strategies for elaborating programs aimed to control or eradicate diseases. Hence, this study aimed to sequence, characterize and compare strains of SRLV, which were isolated from naturally infected goats from the states of Ceará, Rio Grande do Norte and Minas Gerais, Brazil, based on total nucleotide sequences of gag and pol genes. SRLV isolates from the microbiological collection of Sheep and Goat of Embrapa were cultured in goat synovial membrane. Supernatants containing cells were stored and viral particles were purified with sucrose density gradient centrifugation for RNA extraction. Then, sequencing in HiSeq 2500 platform (Illumina) was performed to obtain the total genome of the SRLV strains. The software MEGA version 7.0 was used for sequence editing and phylogeny analysis. A total of 17 isolates were multiplied in cellular culture and six were viable, which were two from Minas Gerais (MG), one from Rio Grande do Norte (RN) and three from Ceará (CE). Two sequences were obtained from the sequencing by synthesis, one from MG (BRMG CNPC) and one from RN (BRRN CNPC). From the total SRLV genome (9,200 bases), sample BRMG CNPC generated a consensus of 6,378 bases, approximately 69,34% of the viral genome and sample BR... Mostrar Tudo |
Palavras-Chave: |
Caracterização molecular; Molecular characterization; SRLV. |
Thesaurus NAL: |
Genome; Goats; Lentivirus; Phylogeny; Sheep. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 03206naa a2200337 a 4500 001 2112529 005 2019-11-07 008 2019 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.smallrumres.2019.06.011$2DOI 100 1 $aAZEVEDO, D. A. A. de 245 $aMolecular characterization of circulating strains of small ruminant lentiviruses in Brazil based on complete gag and pol genes.$h[electronic resource] 260 $c2019 520 $aAbstract: Small ruminant lentiviruses (SRLV) are widely distributed in the world and cause productive and economic losses. These pathogens have high genetic variability, which hinders diagnosis by molecular methods. Molecular characterization studies are good strategies for elaborating programs aimed to control or eradicate diseases. Hence, this study aimed to sequence, characterize and compare strains of SRLV, which were isolated from naturally infected goats from the states of Ceará, Rio Grande do Norte and Minas Gerais, Brazil, based on total nucleotide sequences of gag and pol genes. SRLV isolates from the microbiological collection of Sheep and Goat of Embrapa were cultured in goat synovial membrane. Supernatants containing cells were stored and viral particles were purified with sucrose density gradient centrifugation for RNA extraction. Then, sequencing in HiSeq 2500 platform (Illumina) was performed to obtain the total genome of the SRLV strains. The software MEGA version 7.0 was used for sequence editing and phylogeny analysis. A total of 17 isolates were multiplied in cellular culture and six were viable, which were two from Minas Gerais (MG), one from Rio Grande do Norte (RN) and three from Ceará (CE). Two sequences were obtained from the sequencing by synthesis, one from MG (BRMG CNPC) and one from RN (BRRN CNPC). From the total SRLV genome (9,200 bases), sample BRMG CNPC generated a consensus of 6,378 bases, approximately 69,34% of the viral genome and sample BRRN CNPC generated a consensus of 5,351 bases, which comprised 58.16% of the total genome. Samples in this study demonstrated closer phylogenetic relation with genotype B (B1 group). The remaining strains sequenced in Brazil are also present in the same group with the exception of three samples from genotype A. Immunodominant regions of gag gene and motifs of pol gene were similar to the standard CAEV Cork strain. For the first time, the complete gag and pol genes were sequenced in Brazil from the strain BRMG CNPC isolated in Minas Gerais state. In addition, 2490 bases from the total of 3316 bases were sequenced from the pol gene of the BRRN CNPC sample from Rio Grande do Norte. 650 $aGenome 650 $aGoats 650 $aLentivirus 650 $aPhylogeny 650 $aSheep 653 $aCaracterização molecular 653 $aMolecular characterization 653 $aSRLV 700 1 $aMONTEIRO, J. P. 700 1 $aPINHEIRO, R. R. 700 1 $aMUDADU, M. de A. 700 1 $aANDRIOLI, A. 700 1 $aARAÚJO, J. F. 700 1 $aSOUSA, A. L. M. de 700 1 $aSIDER, L. H. 700 1 $aPEIXOTO, R. M. 700 1 $aTEIXEIRA, M. F. da S. 773 $tSmall Ruminant Research$gv. 177, p. 160-166, 2019.
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