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Registro Completo |
Biblioteca(s): |
Embrapa Agropecuária Oeste; Embrapa Clima Temperado. |
Data corrente: |
16/12/1997 |
Data da última atualização: |
09/08/2022 |
Tipo da produção científica: |
Circular Técnica |
Autoria: |
SACHS, S.; HERTER, F. G.; NAKASU, B. H.; RASEIRA, M. do C. B.; FELICIANO, A. J.; CAMELATTO, D.; MEDEIROS, A. R. M. de; RASEIRA, A.; FONSECA, V. O. da; PEREIRA, J. F. M.; FINARDI, N. L.; MAGNANI, M.; FEHN, L. M.; SALLES, L. A. B. de; FELICIANO, A.; FLORES CANTILLANO, R. F.; SPERRY, S. |
Afiliação: |
SERGIO SACHS, CPACT; FLAVIO GILBERTO HERTER, CPACT; BONIFACIO HIDEYUKI NAKASU, CPACT; MARIA DO CARMO BASSOLS RASEIRA, CPACT; ASCUNIA JIMENEZ FELICIANO, CPACT; DARCY CAMELATTO, CPACT; ANTONIO ROBERTO M DE MEDEIROS, CPACT; AILTON RASEIRA, CPACT; VERA OSORIO DA FONSECA; JOSE FRANCISCO MARTINS PEREIRA, CPACT; NELSON LUIZ FINARDI, CPACT; MARCIO MAGNANI, CPACT; LICELMA MARTINS FEHN, CPACT; LUIZ ANTONIO BENINCA DE SALLES, CPACT; ALBERTO FELICIANO, CPACT; RUFINO FERNANDO FLORES CANTILLANO, CPACT; SUZANA SPERRY, CPAC. |
Título: |
A cultura do pessegueiro. |
Ano de publicação: |
1984 |
Fonte/Imprenta: |
Pelotas: EMBRAPA-CNPFT, 1984. |
Páginas: |
156 p. |
Série: |
(EMBRAPA-CNPFT. Circular Técnica, 10). |
ISSN: |
0100-8110 |
Idioma: |
Português |
Conteúdo: |
Apresentação; Introdução; Localização do pomar; Cultivares; Propagação; Instalação do pomar; Tratos culturais; Poda; Raleio; Adubação e correção do solo; Pragas; Doenças; Guias de tratamentos fitossanitários; Colheita, classificação, embalagem e armazenagem; Bibliografia brasileira do pêssego. |
Palavras-Chave: |
Centro Pesquisa Agropecuaria de Clima Temperado; Controle; Cultivar; EMBRAPA; Fertilizacao; Instalacao; Peach; Propagacao. |
Thesagro: |
Armazenamento; Colheita; Doença; Embalagem; Pêssego; Poda; Pomar; Praga; Prunus Persica; Raleio. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01677nam a2200553 a 4500 001 1742994 005 2022-08-09 008 1984 bl uuuu 00u1 u #d 022 $a0100-8110 100 1 $aSACHS, S. 245 $aA cultura do pessegueiro. 260 $aPelotas: EMBRAPA-CNPFT$c1984 300 $a156 p. 490 $a(EMBRAPA-CNPFT. Circular Técnica, 10). 520 $aApresentação; Introdução; Localização do pomar; Cultivares; Propagação; Instalação do pomar; Tratos culturais; Poda; Raleio; Adubação e correção do solo; Pragas; Doenças; Guias de tratamentos fitossanitários; Colheita, classificação, embalagem e armazenagem; Bibliografia brasileira do pêssego. 650 $aArmazenamento 650 $aColheita 650 $aDoença 650 $aEmbalagem 650 $aPêssego 650 $aPoda 650 $aPomar 650 $aPraga 650 $aPrunus Persica 650 $aRaleio 653 $aCentro Pesquisa Agropecuaria de Clima Temperado 653 $aControle 653 $aCultivar 653 $aEMBRAPA 653 $aFertilizacao 653 $aInstalacao 653 $aPeach 653 $aPropagacao 700 1 $aHERTER, F. G. 700 1 $aNAKASU, B. H. 700 1 $aRASEIRA, M. do C. B. 700 1 $aFELICIANO, A. J. 700 1 $aCAMELATTO, D. 700 1 $aMEDEIROS, A. R. M. de 700 1 $aRASEIRA, A. 700 1 $aFONSECA, V. O. da 700 1 $aPEREIRA, J. F. M. 700 1 $aFINARDI, N. L. 700 1 $aMAGNANI, M. 700 1 $aFEHN, L. M. 700 1 $aSALLES, L. A. B. de 700 1 $aFELICIANO, A. 700 1 $aFLORES CANTILLANO, R. F. 700 1 $aSPERRY, S.
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Registro original: |
Embrapa Clima Temperado (CPACT) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
09/12/2019 |
Data da última atualização: |
09/12/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SANTOS, J. M. L. dos; VASCONCELOS, J. F.; FROTA, G. A.; FREITAS, E. P. de; TEIXEIRA, M.; VIEIRA, L. da S.; BEVILAQUA, C. M. L.; MONTEIRO, J. P. |
Afiliação: |
JESSICA MARIA LEITE DOS SANTOS, Pós-graduação - INTA / UNINTA - Sobral, CE, Brazil; JANAÉLIA FERREIRA VASCONCELOS, Pós-graduação - INTA / UNINTA - Sobral, CE, Brazil; GRACIELLE ARAÚJO FROTA, Pós-graduação - INTA / UNINTA - Sobral, CE, Brazil; EDILSON PEREIRA DE FREITAS, Pós-graduação - INTA / UNINTA - Sobral, CE, Brazil; MARCEL TEIXEIRA, CNPC; LUIZ DA SILVA VIEIRA, CNPC; CLAUDIA MARIA LEAL BEVILAQUA, Pós-graduação - Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brazil; JOMAR PATRICIO MONTEIRO, CNPC. |
Título: |
Quantitative molecular diagnosis of levamisole resistance in populations of Haemonchus contortus. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Experimental Parasitology, v. 205, article 107734, Aug. 2019. |
DOI: |
http://dx.doi.org/10.1016/j.exppara.2019.107734 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Parasitism by Haemonchus contortus is one of the main limiting factors in small ruminant production around the globe. Although several studies suggest the use of integrated management practices, these parasites have been controlled essentially with synthetic anthelmintic drugs. The resistance mechanism against the imidazothiazole derivative levamisole in Haemonchus contortus has not been fully described. Recently, resistance was associated with a 63bp deletion in the Hco-acr-8b gene that encodes a subunit for a nicotinic acetylcholine receptor. This study aimed to standardize a real time PCR (qPCR) protocol for levamisole resistance diagnosis in H. contortus populations based on this polymorphism and use it to characterize 23 field H. contortus populations obtained from different localities of Ceará State, Northeast Brazil. In addition, two populations of H. contortus were used as a standard of susceptibility and resistance, Inbred Strain Edinburgh (ISE) and Kokstad, respectively. Larval development tests (LDT) were performed on five field isolates and both EC50 and EC95 were estimated. LDT EC95 values provided a wider interval between susceptible and resistant populations than EC50 values (EC95?=?1.96-57.93??M; EC50?=?0.05-0.39??M), and were found to be more appropriate for differentiating them. Real time PCR results showed resistance allele frequencies ranged from 20.9 to 76.7%. Our results suggest that levamisole resistance may be present in field populations but it is not as widespread as benzimidazole resistance. This methodology may be useful to monitor levamisole resistance in field populations of H. contortus. MenosAbstract: Parasitism by Haemonchus contortus is one of the main limiting factors in small ruminant production around the globe. Although several studies suggest the use of integrated management practices, these parasites have been controlled essentially with synthetic anthelmintic drugs. The resistance mechanism against the imidazothiazole derivative levamisole in Haemonchus contortus has not been fully described. Recently, resistance was associated with a 63bp deletion in the Hco-acr-8b gene that encodes a subunit for a nicotinic acetylcholine receptor. This study aimed to standardize a real time PCR (qPCR) protocol for levamisole resistance diagnosis in H. contortus populations based on this polymorphism and use it to characterize 23 field H. contortus populations obtained from different localities of Ceará State, Northeast Brazil. In addition, two populations of H. contortus were used as a standard of susceptibility and resistance, Inbred Strain Edinburgh (ISE) and Kokstad, respectively. Larval development tests (LDT) were performed on five field isolates and both EC50 and EC95 were estimated. LDT EC95 values provided a wider interval between susceptible and resistant populations than EC50 values (EC95?=?1.96-57.93??M; EC50?=?0.05-0.39??M), and were found to be more appropriate for differentiating them. Real time PCR results showed resistance allele frequencies ranged from 20.9 to 76.7%. Our results suggest that levamisole resistance may be present in field populations bu... Mostrar Tudo |
Palavras-Chave: |
Anthelmintic resistance; Antinematodal Agents; Drug effects; Haemonchiasis; Nematode parasite; Real-time PCR. |
Thesaurus NAL: |
Benzimidazoles; Drug resistance; Gene frequency; Genetics; Helminths; Levamisole; Nematode larvae; Parasitology; Pharmacology; Sequence alignment; Sheep diseases; Tetramisole. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02921naa a2200433 a 4500 001 2116311 005 2019-12-09 008 2019 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.1016/j.exppara.2019.107734$2DOI 100 1 $aSANTOS, J. M. L. dos 245 $aQuantitative molecular diagnosis of levamisole resistance in populations of Haemonchus contortus.$h[electronic resource] 260 $c2019 520 $aAbstract: Parasitism by Haemonchus contortus is one of the main limiting factors in small ruminant production around the globe. Although several studies suggest the use of integrated management practices, these parasites have been controlled essentially with synthetic anthelmintic drugs. The resistance mechanism against the imidazothiazole derivative levamisole in Haemonchus contortus has not been fully described. Recently, resistance was associated with a 63bp deletion in the Hco-acr-8b gene that encodes a subunit for a nicotinic acetylcholine receptor. This study aimed to standardize a real time PCR (qPCR) protocol for levamisole resistance diagnosis in H. contortus populations based on this polymorphism and use it to characterize 23 field H. contortus populations obtained from different localities of Ceará State, Northeast Brazil. In addition, two populations of H. contortus were used as a standard of susceptibility and resistance, Inbred Strain Edinburgh (ISE) and Kokstad, respectively. Larval development tests (LDT) were performed on five field isolates and both EC50 and EC95 were estimated. LDT EC95 values provided a wider interval between susceptible and resistant populations than EC50 values (EC95?=?1.96-57.93??M; EC50?=?0.05-0.39??M), and were found to be more appropriate for differentiating them. Real time PCR results showed resistance allele frequencies ranged from 20.9 to 76.7%. Our results suggest that levamisole resistance may be present in field populations but it is not as widespread as benzimidazole resistance. This methodology may be useful to monitor levamisole resistance in field populations of H. contortus. 650 $aBenzimidazoles 650 $aDrug resistance 650 $aGene frequency 650 $aGenetics 650 $aHelminths 650 $aLevamisole 650 $aNematode larvae 650 $aParasitology 650 $aPharmacology 650 $aSequence alignment 650 $aSheep diseases 650 $aTetramisole 653 $aAnthelmintic resistance 653 $aAntinematodal Agents 653 $aDrug effects 653 $aHaemonchiasis 653 $aNematode parasite 653 $aReal-time PCR 700 1 $aVASCONCELOS, J. F. 700 1 $aFROTA, G. A. 700 1 $aFREITAS, E. P. de 700 1 $aTEIXEIRA, M. 700 1 $aVIEIRA, L. da S. 700 1 $aBEVILAQUA, C. M. L. 700 1 $aMONTEIRO, J. P. 773 $tExperimental Parasitology$gv. 205, article 107734, Aug. 2019.
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