Data/hora: 22/06/2024 17:27:54

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 20/06/2018
Data da última atualização: | 20/06/2018
Autoria: | OLIVEIRA, A. P. L.; RANGEL, J. P. P.; RAPOSO, V.; PIANCA, N. G.; CRUZ, E. P.; PEREIRA NETO, E.; FIORIO, W. A. B.; MONTEIRO, B. S.
Afiliação: | Alvaro P. L. Oliveira, Laboratório de Terapia Celular e Células Tronco/Departamento de Medicina Veterinária/Universidade Vila Velha - UVV; Julia P. P. Rangel, Laboratório de Terapia Celular e Células Tronco/Departamento de Medicina Veterinária/Universidade Vila Velha - UVV; Viviane Raposo, Laboratório de Terapia Celular e Células/Departamento de Medicina Veterinária - UVV; Noely G. Pianca, Iniciação Científica/Laboratório de Terapia Celular e Células/Departamento de Medicina Veterinária - UVV; Emanuelle P. Cruz, Iniciação Científica/Laboratório de Terapia Celular e Células/Departamento de Medicina Veterinária - UVV; Evandro Pereira Neto, Laboratório de Terapia Celular e Células Tronco/Departamento de Medicina Veterinária/Universidade Vila Velha - UVV; Wagner A. B. Fiorio, Laboratório de Terapia Celular e Células Tronco/Departamento de Medicina Veterinária/Universidade Vila Velha - UVV; Betânia S. Monteiro, Laboratório de Terapia Celular e Células/Departamento de Medicina Veterinária - UVV.
Título: | Allogenic mesenchymal stem cell intravenous infusion in reparation of mild intestinal ischemia/reperfusion injury in New Zealand rabbits.
Ano de publicação: | 2018
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 4, p. 710-721, abril 2018
Idioma: | Inglês
Notas: | Título em português: Infusão intravenosa de células tronco mesenquimais alógenas na reparação da lesão branda de isquemia/reperfusão intestinal em coelhos Nova Zelândia.
Conteúdo: | The present study aimed to evaluate the efficacy of mesenchymal stem cell (MSC) infusion, derived from adipose tissue, on reduction of local and remote tissue damage caused by the event of experimental intestinal I/R in New Zealand breed rabbits. For obtaining, characterization, and cultivation of MSC derived from adipose tissue (MSC-Adp), 3 juvenile animals (four months old) were used. The cells were considered to be viable for therapy after the fourth passage (in vitro phase). For the in vivo stage, 24 young adult animals (six months old) were used, weighing approximately 3.5 kg, in which were randomly divided into two groups, called: IR treated with MSC (I2H/R5H MSC 3D; I2H/R5H MSC 7D); IR treated with PBS (I2H/R5H PBS 3D; I2H/R5H PBS 7D). The animals were anesthetized and submitted to pre-retro-umbilical midline celiotomy. The extramural peri-intestinal marginal artery was located and clamped (predetermined and standardized region) with the aid of a vascular clip, promoting a 2 hour blood flow interruption. After this period, blood flow was reestablished, inhalatory anesthesia was suspended, and the animals awaken. After 5 hours of reperfusion, the treatments were performed by intravenous infusion according to the experimental groups. The animals were evaluated 72 hours and seven days after the treatment as for the macroscopic appearance (color and peristaltism) of the jejunal segment, and by histological evaluation of the ischemic segment for the presence or absence of destruction of the intestinal mucosa, edema, bleeding, dilation of lymph vessels, and presence of polymorphonuclear inflammatory cells, both in the mucosa and submucosa. The observed results revealed that the groups treated with MSC-Adp obtained smaller mucosal and submucosal lesions when compared to the groups treated with PBS. Also, MSC-Adp treated groups obtained controlled inflammatory response and higher mitotic rate, outcomes related to the therapeutic potential of MSC. Infusion of stem cells attenuated the lesions caused by intestinal I/R in both MSC groups when compared to the group treated with PBS.
Palavras-Chave: | Artéria marginal peri-intestinal extramural; Cell therapy; Células tronco mesenquimais; Clínica; Extramural peri-intestinal; Intestinal ischemia; Intravenous infusion; Isquemia intestinal; Lesão remota; New Zealand rabbit; Remote injury; Terapia celular.
Thesagro: | Coelho Nova Zelândia.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/178809/1/Allogenic-mesenchymal-stem-cell-intravenous-infusion.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (62736-1) - UPE - AP - DD -  -  -  - DIS