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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/08/2020 |
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Data da última atualização: |
25/08/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARENA, G. D.; RAMOS-GONZALEZ, P. L.; FALK, B. W.; CASTEEL, C. L.; ASTUA, J. de F.; MACHADO, M. A. |
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Afiliação: |
GABRIELLA D. ARENA, Centro de Citricultura Sylvio Moreira; PEDRO LUIS RAMOS-GONZALEZ, Instituto Biológico; BRYCE W. FALK, University of California; CLARE L. CASTEEL, CASTEEL; JULIANA DE FREITAS ASTUA, CNPMF; MARCOS A. MACHADO, Centro de Citricultura Sylvio Moreira. |
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Título: |
Plant immune system activation upon Citrus Leprosis Virus c infection is mimicked by the ectopic expression of the P61 viral protein. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Frontiers in Plant Science, August, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae) is an atypical virus that does not spread systemically in its plant hosts. Upon its inoculation by Brevipalpus mites, only localized lesions occur, and the infection remains limited to cells around mite feeding sites. Here, we aimed to gain insights into the putative causes of viral unfitness in plants by expanding the limited knowledge of the molecular mechanisms underlying plant/kitavirid interactions. Firstly, we quantified the CiLV-C viral RNAs during the infection in Arabidopsis thaliana plants using RT-qPCR and systematized it by defining three stages of distinguishing subgenomic and genomic RNA accumulation: i) 0–24 h after infestation, ii) 2–4 days after infestation (dai), and iii) 6–10 dai. Accordingly, the global plant response to CiLV-C infection was assessed by RNA-Seq at each period. Results indicated a progressive reprogramming of the plant transcriptome in parallel to the increasing viral loads. Gene ontology enrichment analysis revealed the induction of cell growth-related processes at the early stages of the infection and the triggering of the SA-mediated pathway, ROS burst and hypersensitive response (HR) at the presymptomatic stage. Conversely, infected plants downregulated JA/ET-mediated pathways and processes involved in the primary metabolism including photosynthesis. Marker genes of unfolded protein response were also induced, suggesting a contribution of the endoplasmic reticulum stress to the cell death caused by the viral infection. Finally, we transiently expressed CiLV-C proteins in Nicotiana benthamiana plants to undertake their roles in the elicited plant responses. Expression of the CiLV-C P61 protein consistently triggered ROS burst, upregulated SA- and HR-related genes, increased SA levels, reduced JA levels, and caused cell death. Mimicry of responses typically observed during CiLV-C–plant interaction indicates P61 as a putative viral effector causing the HR-like symptoms associated with the infection. Our data strengthen the hypothesis that symptoms of CiLV-C infection might be the outcome of a hypersensitive-like response during an incompatible interaction. Consequently, the locally restricted infection of CiLV-C, commonly observed across infections by kitavirids, supports the thesis that these viruses, likely arising from an ancestral arthropod-infecting virus, are unable to fully circumvent plant defenses. MenosCitrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae) is an atypical virus that does not spread systemically in its plant hosts. Upon its inoculation by Brevipalpus mites, only localized lesions occur, and the infection remains limited to cells around mite feeding sites. Here, we aimed to gain insights into the putative causes of viral unfitness in plants by expanding the limited knowledge of the molecular mechanisms underlying plant/kitavirid interactions. Firstly, we quantified the CiLV-C viral RNAs during the infection in Arabidopsis thaliana plants using RT-qPCR and systematized it by defining three stages of distinguishing subgenomic and genomic RNA accumulation: i) 0–24 h after infestation, ii) 2–4 days after infestation (dai), and iii) 6–10 dai. Accordingly, the global plant response to CiLV-C infection was assessed by RNA-Seq at each period. Results indicated a progressive reprogramming of the plant transcriptome in parallel to the increasing viral loads. Gene ontology enrichment analysis revealed the induction of cell growth-related processes at the early stages of the infection and the triggering of the SA-mediated pathway, ROS burst and hypersensitive response (HR) at the presymptomatic stage. Conversely, infected plants downregulated JA/ET-mediated pathways and processes involved in the primary metabolism including photosynthesis. Marker genes of unfolded protein response were also induced, suggesting a contribution of the endoplasmic reticulum st... Mostrar Tudo |
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LEADER 03074naa a2200193 a 4500
001 2124551
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008 2020 bl uuuu u00u1 u #d
100 1 $aARENA, G. D.
245 $aPlant immune system activation upon Citrus Leprosis Virus c infection is mimicked by the ectopic expression of the P61 viral protein.$h[electronic resource]
260 $c2020
520 $aCitrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae) is an atypical virus that does not spread systemically in its plant hosts. Upon its inoculation by Brevipalpus mites, only localized lesions occur, and the infection remains limited to cells around mite feeding sites. Here, we aimed to gain insights into the putative causes of viral unfitness in plants by expanding the limited knowledge of the molecular mechanisms underlying plant/kitavirid interactions. Firstly, we quantified the CiLV-C viral RNAs during the infection in Arabidopsis thaliana plants using RT-qPCR and systematized it by defining three stages of distinguishing subgenomic and genomic RNA accumulation: i) 0–24 h after infestation, ii) 2–4 days after infestation (dai), and iii) 6–10 dai. Accordingly, the global plant response to CiLV-C infection was assessed by RNA-Seq at each period. Results indicated a progressive reprogramming of the plant transcriptome in parallel to the increasing viral loads. Gene ontology enrichment analysis revealed the induction of cell growth-related processes at the early stages of the infection and the triggering of the SA-mediated pathway, ROS burst and hypersensitive response (HR) at the presymptomatic stage. Conversely, infected plants downregulated JA/ET-mediated pathways and processes involved in the primary metabolism including photosynthesis. Marker genes of unfolded protein response were also induced, suggesting a contribution of the endoplasmic reticulum stress to the cell death caused by the viral infection. Finally, we transiently expressed CiLV-C proteins in Nicotiana benthamiana plants to undertake their roles in the elicited plant responses. Expression of the CiLV-C P61 protein consistently triggered ROS burst, upregulated SA- and HR-related genes, increased SA levels, reduced JA levels, and caused cell death. Mimicry of responses typically observed during CiLV-C–plant interaction indicates P61 as a putative viral effector causing the HR-like symptoms associated with the infection. Our data strengthen the hypothesis that symptoms of CiLV-C infection might be the outcome of a hypersensitive-like response during an incompatible interaction. Consequently, the locally restricted infection of CiLV-C, commonly observed across infections by kitavirids, supports the thesis that these viruses, likely arising from an ancestral arthropod-infecting virus, are unable to fully circumvent plant defenses.
650 $aFruta Cítrica
700 1 $aRAMOS-GONZALEZ, P. L.
700 1 $aFALK, B. W.
700 1 $aCASTEEL, C. L.
700 1 $aASTUA, J. de F.
700 1 $aMACHADO, M. A.
773 $tFrontiers in Plant Science, August, 2020.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 51 | |
| 22. |  | ARENA, G. D.; RAMOS-GONZÁLEZ, P. L.; NUNES, M. A.; JESUS, C. C.; CALEGARIO, R. F.; KITAJIMA, E. W.; NOVELLI, V. M.; ASTUA, J. de F. Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses. Scientia Agricola, v.74, n.1, p.1-10X, 2017| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 23. | | RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; GUERRA-PERAZA, O.; TASSI, A. D.; KITAJIMA, E. W.; HARAKAVA, R.; SALAROLI, R. B.; ASTUA, J. de F. Citrus leprosis virus N: a new dichorhavirus causing citrus leprosis disease. Phytopathology, v.107, n.8, August 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 24. | | ASTUA, J. de F.; JESUS, C. C. de; RAMOS-GONZÁLEZ, P. L.; NOVELLI, V. M.; BASTIANEL, M.; NUNES, M. A.; GUERRA-PERAZA, O.; KITAJIMA, E. W. O complexo leprose dos citros: uma doença com muitos agentes causais. Citricultura Atual, Cordeiropolis-SP, v. 20, n. 109, p. 20-22, mai., 2016. 20-22| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 25. | | SIVIERO, A.; MATTAR, E. P. L.; CAMELO-GARCIA, V.; RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; SANDE, O. F. L.; ZERBINI, F. M.; KITAJIMA, E. W. Levantamento de vírus de plantas no Estado do Acre. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 51., 2109, Recife. Os avanços da Fitopatologia na Era Genômica: anais. Recife: SBF: UFPRE/PPGF, 2019. p. 776.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| 26. | | ARENA, G. D.; RAMOS-GONZÁLEZ, P. L.; ROGERIO, L. A.; RIBEIRO-ALVES, M.; CASTEEL, C. L.; ASTUA, J. de F.; MACHADO, M. A. Making a better home: modulation of plant defensive response by brevipalpus mites. Frontiers in Plant Science, v.9, August, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 27. | | CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; TASSI, A. D.; GUERRA-PERAZA, O.; KITAJIMA, E. W.; HARAKAVA, R.; BESERRA, JR. J. E. A.; ASTUA, J. de F. Identification and characterization of citrus chlorotic spot virus, a new dichorhavirus associated with citrus leprosis-like symptoms. Plant Disease, v.102, p. 1588-1598, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 28. | | DIETZGEN, R. G.; ASTUA, J. de F.; CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; GOODIN, M. M.; KONDO, H.; TASSI, A. D.; KITAJIMA, E. W. Dichorhaviruses in their host plants and mite vectors. Advances in Virus Research, Elsevier, 2018. 30p. il.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 29. | | CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; TASSI, A. D.; BARGUIL, B. M.; BESERRA JUNIOR, J. E. A.; HARAKAVA, R.; KITAJIMA, E. W.; ASTUA, J. de F. First Report of Citrus Chlorotic Spot Virus Infecting the Succulent Plant Agave desmettiana. Plant Disease, v.103, n. 6, June 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 30. | | SAUVÊTRE, P.; VENIANT, E.; CROQ, G.; TASSI, A. D.; KITAJIMA, E. W.; CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; ASTUA, J. de F.; FERREIRA, D. N. M. First report of orchid fleck virus in the orchid collection of Jardin du Luxembourg, Paris, France. Plant Disease, v. 102, n. 12, p. 2670, 2018. Na publicação: J. Freitas-Astúa, D. Navia.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 31. | | COSTA-RODRIGUES, M. da; PEREIRA, L. R.; RAMOS-GONZÁLEZ, P. L.; TASSI, D.; LEÃO, I. G.; HARAKAVA, R.; KITAJIMA, E. W.; ASTUA, J. de F. First report of Passion fruit green spot virusinfecting hibiscus plants. New Disease Report, v.45, n.2, e12080, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 33. | | RODRIGUES, M. da C.; RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; HARAKAVA, R.; BARBOSA, C. de J.; COSTA, H.; ASTUA, J. de F. Primeiro relato de um higrevirus na área Continental Americana.first report of a Higrevirus in Mainland America. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 52., 2021. Anais... [Brasília, DF]: Sociedade Brasileira de Fitopatologia, 2021. p. 182. Evento online.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 34. | | CHABI-JESUS C.; RAMOS-GONZÁLEZ, P. L.; GUERRA-PERAZA, O.; HARAKAVA, R.; KITAJIMA, E. W.; BASTIANEL, M.; ASTUA, J. de F. Disclosing three citrus leprosis-associated viruses of the new genus Dichorhavirus, family rhabdoviridae. In: INTERNATIONAL CITRUS CONGRESS, 2016, Foz do Iguaçu. Abstract book. Campinas: IAC; Londrina: IAPAR, 2016. p.215. Resumo S15-372.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 38. | | KITAJIMA, E. W.; RAMOS-GONZÁLEZ, P. L.; ASTUA, J. de F.; ALEXANDRE, M. A. V.; PIRES, G. R. R.; CHABI-JESUS, C.; TASSI, A. D.; CALEGARIO, R. F. Novas espécies de plantas hospedeiras naturalmente infectadas por vírus transmitidos por Ácaros Brevipalpus no Brasil new plant host species naturally infected by brevipalpus-transmitted viruses in Brazil. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 52., 2021. Anais... [Brasília, DF]: Sociedade Brasileira de Fitopatologia, 2021. p. 174. Evento online.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 39. | | RAMOS-GONZÁLEZ, P. L.; CHABI-JESUS, C.; TASSI, A. D.; CALEGARIO, R. F.; HARAKAVA, R.; NOME, C F.; KITAJIMA, E. W.; ASTUA, J. de F. A Novel Lineage of Cile-Like Viruses Discloses the Phylogenetic Continuum Across the Family Kitaviridae. Frontiers in Microbiology, v.28, n.13, 836076, March 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 40. | | CHABI-JESUS, C.; NAJAR, A.; FONTENELE, R. S.; KUMARI, S. G.; RAMOS-GONZÁLEZ, P. L.; ASTUA, J. de F.; KRABERGER, S.; VARSANI, A. Viruses representing two new genomovirus species identified in citrus from Tunisia. Archives of Virology,Springer-Verlag GmbH Austria, March, 2020. 5 p. il.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 51 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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