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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
25/08/2020 |
Data da última atualização: |
25/08/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ARENA, G. D.; RAMOS-GONZALEZ, P. L.; FALK, B. W.; CASTEEL, C. L.; ASTUA, J. de F.; MACHADO, M. A. |
Afiliação: |
GABRIELLA D. ARENA, Centro de Citricultura Sylvio Moreira; PEDRO LUIS RAMOS-GONZALEZ, Instituto Biológico; BRYCE W. FALK, University of California; CLARE L. CASTEEL, CASTEEL; JULIANA DE FREITAS ASTUA, CNPMF; MARCOS A. MACHADO, Centro de Citricultura Sylvio Moreira. |
Título: |
Plant immune system activation upon Citrus Leprosis Virus c infection is mimicked by the ectopic expression of the P61 viral protein. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Frontiers in Plant Science, August, 2020. |
Idioma: |
Inglês |
Conteúdo: |
Citrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae) is an atypical virus that does not spread systemically in its plant hosts. Upon its inoculation by Brevipalpus mites, only localized lesions occur, and the infection remains limited to cells around mite feeding sites. Here, we aimed to gain insights into the putative causes of viral unfitness in plants by expanding the limited knowledge of the molecular mechanisms underlying plant/kitavirid interactions. Firstly, we quantified the CiLV-C viral RNAs during the infection in Arabidopsis thaliana plants using RT-qPCR and systematized it by defining three stages of distinguishing subgenomic and genomic RNA accumulation: i) 0–24 h after infestation, ii) 2–4 days after infestation (dai), and iii) 6–10 dai. Accordingly, the global plant response to CiLV-C infection was assessed by RNA-Seq at each period. Results indicated a progressive reprogramming of the plant transcriptome in parallel to the increasing viral loads. Gene ontology enrichment analysis revealed the induction of cell growth-related processes at the early stages of the infection and the triggering of the SA-mediated pathway, ROS burst and hypersensitive response (HR) at the presymptomatic stage. Conversely, infected plants downregulated JA/ET-mediated pathways and processes involved in the primary metabolism including photosynthesis. Marker genes of unfolded protein response were also induced, suggesting a contribution of the endoplasmic reticulum stress to the cell death caused by the viral infection. Finally, we transiently expressed CiLV-C proteins in Nicotiana benthamiana plants to undertake their roles in the elicited plant responses. Expression of the CiLV-C P61 protein consistently triggered ROS burst, upregulated SA- and HR-related genes, increased SA levels, reduced JA levels, and caused cell death. Mimicry of responses typically observed during CiLV-C–plant interaction indicates P61 as a putative viral effector causing the HR-like symptoms associated with the infection. Our data strengthen the hypothesis that symptoms of CiLV-C infection might be the outcome of a hypersensitive-like response during an incompatible interaction. Consequently, the locally restricted infection of CiLV-C, commonly observed across infections by kitavirids, supports the thesis that these viruses, likely arising from an ancestral arthropod-infecting virus, are unable to fully circumvent plant defenses. MenosCitrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae) is an atypical virus that does not spread systemically in its plant hosts. Upon its inoculation by Brevipalpus mites, only localized lesions occur, and the infection remains limited to cells around mite feeding sites. Here, we aimed to gain insights into the putative causes of viral unfitness in plants by expanding the limited knowledge of the molecular mechanisms underlying plant/kitavirid interactions. Firstly, we quantified the CiLV-C viral RNAs during the infection in Arabidopsis thaliana plants using RT-qPCR and systematized it by defining three stages of distinguishing subgenomic and genomic RNA accumulation: i) 0–24 h after infestation, ii) 2–4 days after infestation (dai), and iii) 6–10 dai. Accordingly, the global plant response to CiLV-C infection was assessed by RNA-Seq at each period. Results indicated a progressive reprogramming of the plant transcriptome in parallel to the increasing viral loads. Gene ontology enrichment analysis revealed the induction of cell growth-related processes at the early stages of the infection and the triggering of the SA-mediated pathway, ROS burst and hypersensitive response (HR) at the presymptomatic stage. Conversely, infected plants downregulated JA/ET-mediated pathways and processes involved in the primary metabolism including photosynthesis. Marker genes of unfolded protein response were also induced, suggesting a contribution of the endoplasmic reticulum st... Mostrar Tudo |
Thesagro: |
Fruta Cítrica. |
Categoria do assunto: |
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LEADER 03074naa a2200193 a 4500 001 2124551 005 2020-08-25 008 2020 bl uuuu u00u1 u #d 100 1 $aARENA, G. D. 245 $aPlant immune system activation upon Citrus Leprosis Virus c infection is mimicked by the ectopic expression of the P61 viral protein.$h[electronic resource] 260 $c2020 520 $aCitrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae) is an atypical virus that does not spread systemically in its plant hosts. Upon its inoculation by Brevipalpus mites, only localized lesions occur, and the infection remains limited to cells around mite feeding sites. Here, we aimed to gain insights into the putative causes of viral unfitness in plants by expanding the limited knowledge of the molecular mechanisms underlying plant/kitavirid interactions. Firstly, we quantified the CiLV-C viral RNAs during the infection in Arabidopsis thaliana plants using RT-qPCR and systematized it by defining three stages of distinguishing subgenomic and genomic RNA accumulation: i) 0–24 h after infestation, ii) 2–4 days after infestation (dai), and iii) 6–10 dai. Accordingly, the global plant response to CiLV-C infection was assessed by RNA-Seq at each period. Results indicated a progressive reprogramming of the plant transcriptome in parallel to the increasing viral loads. Gene ontology enrichment analysis revealed the induction of cell growth-related processes at the early stages of the infection and the triggering of the SA-mediated pathway, ROS burst and hypersensitive response (HR) at the presymptomatic stage. Conversely, infected plants downregulated JA/ET-mediated pathways and processes involved in the primary metabolism including photosynthesis. Marker genes of unfolded protein response were also induced, suggesting a contribution of the endoplasmic reticulum stress to the cell death caused by the viral infection. Finally, we transiently expressed CiLV-C proteins in Nicotiana benthamiana plants to undertake their roles in the elicited plant responses. Expression of the CiLV-C P61 protein consistently triggered ROS burst, upregulated SA- and HR-related genes, increased SA levels, reduced JA levels, and caused cell death. Mimicry of responses typically observed during CiLV-C–plant interaction indicates P61 as a putative viral effector causing the HR-like symptoms associated with the infection. Our data strengthen the hypothesis that symptoms of CiLV-C infection might be the outcome of a hypersensitive-like response during an incompatible interaction. Consequently, the locally restricted infection of CiLV-C, commonly observed across infections by kitavirids, supports the thesis that these viruses, likely arising from an ancestral arthropod-infecting virus, are unable to fully circumvent plant defenses. 650 $aFruta Cítrica 700 1 $aRAMOS-GONZALEZ, P. L. 700 1 $aFALK, B. W. 700 1 $aCASTEEL, C. L. 700 1 $aASTUA, J. de F. 700 1 $aMACHADO, M. A. 773 $tFrontiers in Plant Science, August, 2020.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
15/10/2007 |
Data da última atualização: |
21/10/2009 |
Autoria: |
REATTO, A.; MARTINS, E. de S.; SILVA, A. V. da; CARVALHO JÚNIOR, O. A. de. |
Título: |
Análise da informação pedológica da região de Araguaína e Palmeirante-TO para fins de zoneamento agrícola. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
Planaltina, DF: Embrapa Cerrados, 2005. |
Páginas: |
18 p. |
Série: |
(Embrapa Cerrados. Documentos, 149). |
Idioma: |
Português |
Conteúdo: |
The purpose of this study was report of Research and Development of Embrapa Cerrados about Agricultural Zoning of Tocantins in relation to agricultural expansion in the Araguaína and Palmeirante Area of the State of Tocantins. In general the north area of the State of Tocantins have around 40% of sandy soils with low agricultural potential. These soils request in the minimum cultivation and direct planting. |
Palavras-Chave: |
Mapeamento; Potencial agrícola; Soil sciences; Zoneamento. |
Thesagro: |
Pedologia; Reconhecimento do Solo; Zoneamento Agrícola. |
Thesaurus NAL: |
cartography; soil surveys; zoning. |
Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAC-2009/28005/1/doc_149.pdf
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Marc: |
LEADER 01248nam a2200289 a 4500 001 1570356 005 2009-10-21 008 2005 bl uuuu u0uu1 u #d 100 1 $aREATTO, A. 245 $aAnálise da informação pedológica da região de Araguaína e Palmeirante-TO para fins de zoneamento agrícola. 260 $aPlanaltina, DF: Embrapa Cerrados$c2005 300 $a18 p. 490 $a(Embrapa Cerrados. Documentos, 149). 520 $aThe purpose of this study was report of Research and Development of Embrapa Cerrados about Agricultural Zoning of Tocantins in relation to agricultural expansion in the Araguaína and Palmeirante Area of the State of Tocantins. In general the north area of the State of Tocantins have around 40% of sandy soils with low agricultural potential. These soils request in the minimum cultivation and direct planting. 650 $acartography 650 $asoil surveys 650 $azoning 650 $aPedologia 650 $aReconhecimento do Solo 650 $aZoneamento Agrícola 653 $aMapeamento 653 $aPotencial agrícola 653 $aSoil sciences 653 $aZoneamento 700 1 $aMARTINS, E. de S. 700 1 $aSILVA, A. V. da 700 1 $aCARVALHO JÚNIOR, O. A. de
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