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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
21/01/2004 |
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Data da última atualização: |
21/01/2004 |
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Autoria: |
ANDRÉO, Y.; ZCARELI, C.; RAMOS JÚNIOR, E. U.; NAKAGAWA, J.; RODRIGUES, J. D.; ONO, E. O. |
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Título: |
Citocinina e potencial fisiológico de sementes de milho doce. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Informativo Abrates, Londrina, v. 13, n. 3, p. 307, set. 2003. |
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Idioma: |
Português |
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Notas: |
Trabalho apresentado no 13º Congresso Brasileiro de Sementes, 2003, Londrina. Resumo. |
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Palavras-Chave: |
Citocinina; Cltivar BR401. |
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Thesagro: |
Deterioração; Milho Doce; Semente; Zea Mays. |
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Thesaurus Nal: |
vigor. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00772naa a2200265 a 4500
001 1775210
005 2004-01-21
008 2003 bl uuuu u00u1 u #d
100 1 $aANDRÉO, Y.
245 $aCitocinina e potencial fisiológico de sementes de milho doce.
260 $c2003
500 $aTrabalho apresentado no 13º Congresso Brasileiro de Sementes, 2003, Londrina. Resumo.
650 $avigor
650 $aDeterioração
650 $aMilho Doce
650 $aSemente
650 $aZea Mays
653 $aCitocinina
653 $aCltivar BR401
700 1 $aZCARELI, C.
700 1 $aRAMOS JÚNIOR, E. U.
700 1 $aNAKAGAWA, J.
700 1 $aRODRIGUES, J. D.
700 1 $aONO, E. O.
773 $tInformativo Abrates, Londrina$gv. 13, n. 3, p. 307, set. 2003.
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Registro original: |
Embrapa Hortaliças (CNPH) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
04/03/2016 |
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Data da última atualização: |
04/03/2016 |
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Autoria: |
LEMOS, V. F.; GUARANÁ, E. L. S.; AFONSO, J. A. B.; FAGLIARI, J. J.; SILVA, P. C.; SOARES P. C.; MENDONÇA, C. L. de. |
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Afiliação: |
VÂNIA F. LEMOS, UFRPE; EDUARDO L. S. GUARANÁ, UFRPE; JOSÉ A. B. AFONSO, UFRPE; JOSÉ J. FAGLIARI, UNESP; PAULO C. SILVA, UNESP; PIERRE C. SOARES, UFRPE; CARLA LOPES DE MENDONÇA, UFRPE. |
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Título: |
Effects of intramammary infection on whey proteinograms os sheep during lactation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 3, p. 230-236, mar. 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
The study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), ?-lactalbumin, ?-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and ?-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for IgG LC and ?-lactoalbumin. The most significant changes were observed in ?-1- acid glycoprotein, lactoferrin and haptoglobin, which showed three-, five-, and seven-fold increases in secretion, respectively. This study showed that haptoglobin, ?-1-acid glycoprotein, lactoferrin, albumin, and the IgA and IgG immunoglobulins may serve as potential biomarkers for mammary gland infection in sheep. MenosThe study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), ?-lactalbumin, ?-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and ?-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for ... Mostrar Tudo |
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Palavras-Chave: |
Marcador biológico; Mastite subclínica; Proteína de fase aguda; Subclinical mastitis. |
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Thesagro: |
Leite; Ovelha. |
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Thesaurus NAL: |
Acute phase proteins; Biomarkers; Ewes; Milk. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140638/1/Effects-of-intramammary-infection.pdf
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Marc: |
LEADER 02780naa a2200313 a 4500
001 2039647
005 2016-03-04
008 2015 bl uuuu u00u1 u #d
100 1 $aLEMOS, V. F.
245 $aEffects of intramammary infection on whey proteinograms os sheep during lactation.
260 $c2015
520 $aThe study aimed to identify potential biomarkers of mammary gland infection in Santa Inês sheep. Commercial flocks of sheep provided the same hygiene, sanitary, and nutritional management under semi-intensive production systems were monitored during the lactation stage-and assessed 15, 30, 60, and 90 days after delivery (through the end of lactation and weaning). The California Mastitis Test (CMT) was performed on the mammary glands. Milk was collected for bacterial examination and protein analysis. Bacterial culture and biochemical characterization of the samples were performed. Forty-two milk samples from healthy glands (negative CMT and bacterial testing) and 43 milk samples from infected glands (positive CMT and bacterial testing) taken at the predefined time points were assessed. A rennin solution was used to obtain the whey. The proteins analysis was performed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which allowed for the quantification of nine whey proteins produced in healthy glands: serum albumin, lactoferrin, IgA, IgG heavy-chain (IgG HC), IgG light-chain (IgG LC), total IgG (IgG HC + IgG LC), ?-lactalbumin, ?-lactoglobulin, protein with MW 15.000 Da, protein with MW 29.000 Da and eleven whey proteins secreted by infected glands, including haptoglobin and ?-1-acid glycoprotein. A comparison of whey proteins between healthy and infected glands showed increases (P<0.05) in the secreted and total contents of all proteins, except for IgG LC and ?-lactoalbumin. The most significant changes were observed in ?-1- acid glycoprotein, lactoferrin and haptoglobin, which showed three-, five-, and seven-fold increases in secretion, respectively. This study showed that haptoglobin, ?-1-acid glycoprotein, lactoferrin, albumin, and the IgA and IgG immunoglobulins may serve as potential biomarkers for mammary gland infection in sheep.
650 $aAcute phase proteins
650 $aBiomarkers
650 $aEwes
650 $aMilk
650 $aLeite
650 $aOvelha
653 $aMarcador biológico
653 $aMastite subclínica
653 $aProteína de fase aguda
653 $aSubclinical mastitis
700 1 $aGUARANÁ, E. L. S.
700 1 $aAFONSO, J. A. B.
700 1 $aFAGLIARI, J. J.
700 1 $aSILVA, P. C.
700 1 $aSOARES P. C.
700 1 $aMENDONÇA, C. L. de
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n. 3, p. 230-236, mar. 2015.
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