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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
21/11/2012 |
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Data da última atualização: |
14/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
D'AFONSECA, V.; SOARES, S. C.; ALI, A.; SANTOS, A. R.; PINTO, A. C.; MAGALHÃES, A. A. C.; FARIA, C. de J.; BARBOSA, E.; GUIMARÃES, L. C.; ESLABÃO, M.; ALMEIDA, S. S.; ABREU, V. A. C.; ZERLOTINI, A.; CARNEIRO, A. R.; CERDEIRA, L. T.; RAMOS, R. T. J.; HIRATA JÚNIOR, R.; MATTOS-GUARALDI, A. L.; TROST, E.; TAUCH, A.; SILVA, A.; SCHNEIDER, M. P.; MIYOSHI, A.; AZEVEDO, V. |
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Afiliação: |
VÍVIAN D’AFONSECA, UFMG; SIOMAR C. SOARES, UFMG; AMJAD ALI, UFMG; ANDERSON R. SANTOS, UFMG; ANNE C. PINTO, UFMG; ARYANE A. C. MAGALHÃES, UFMG; CÁSSIO DE JESUS FARIA, UFMG; EUDES BARBOSA, UFMG; LUIS C. GUIMARÃES, UFMG; MARCUS ESLABÃO, UFPel; SINTIA S. ALMEIDA, UFMG; VINICIUS A. C. ABREU, UFMG; ADHEMAR ZERLOTINI NETO, CNPTIA; ADRIANA R. CARNEIRO, UFPA; LOUISE T. CERDEIRA, UFPA; ROMMEL T. J. RAMOS, UFPA; RAPHAEL HIRATA JÚNIOR, UERJ; ANA L. MATTOS-GUARALDI, UERJ; EVA TROST, Bielefeld University; ANDREAS TAUCH, Bielefeld University; ARTUR SILVA, UFPA; MARIA P. SCHNEIDER, UFPA; ANDERSON MIYOSHI, UFMG; VASCO AZEVEDO, UFMG. |
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Título: |
Reannotation of the Corynebacterium diphtheriae NCTC13129 genome as a new approach to studying gene targets connected to virulence and pathogenicity in diphtheria. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Open Access Bioinformatics, v. 4, p. 1-13, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Background: The reannotation of genomes already on file is a new approach to discovering new genetic elements and to make the genomes more descriptive and current with relevant features regarding the organism?s lifestyle. Within this approach, the present study aimed to reannotate the genome of the Gram-positive human pathogen Corynebacterium diphtheriae, which causes diphtheria. The deposit of massive amounts of information linked to other species of the genus Corynebacterium has facilitated the updating of the genomic interpretation of this microorganism. Additionally, the emergence of invasive disease by nontoxigenic strains of C. diphtheriae and the reemergence of diphtheria in partially immunized populations have given impetus to new studies in relation to its structural and functional genome. Results: In relation to structural genomics, 23 coding regions (coding sequences) were deleted and 71 new genes were added to the genome annotation. Nevertheless, all the pseudogenes were validated and ten new pseudogenes were created. In relation to functional genomics, about 57% of the genome annotation was updated and became functionally more informative. The product descriptions of 41% (973 proteins) were updated. Among them, 370 that were previously annotated as ?hypothetical proteins,? now have more informative descriptions. With the new annotation, the plasticity of the genome became evident, which shows improvements in the annotation of 13 pathogenicity islands already described in the literature. In addition, the large number of transposases and the presence of structural genes of bacteriophages make their genomic versatility evident. Contrasting with this reality, it also allowed the clarification of some aspects concerned with mechanisms used by C. diphtheriae to stop the invasion of the genome by bacteriophages, mediated by the clustered regularly interspaced short palindromic repeats region. Conclusion: The reannotation of the C. diphtheriae genome provided an improvement in annotation of the C. diphtheriae genome in several aspects, such as virulence characteristics and plasticity events. Moreover, the protocol used here can be extended to various other pathogens in order to improve the genomic information already on file in public databases and to minimize propagating errors. The reannotated archive and updated archive are available at: http://lgcm.icb.ufmg.br/pub/C_diphtheriae_reannotation.embl. MenosBackground: The reannotation of genomes already on file is a new approach to discovering new genetic elements and to make the genomes more descriptive and current with relevant features regarding the organism?s lifestyle. Within this approach, the present study aimed to reannotate the genome of the Gram-positive human pathogen Corynebacterium diphtheriae, which causes diphtheria. The deposit of massive amounts of information linked to other species of the genus Corynebacterium has facilitated the updating of the genomic interpretation of this microorganism. Additionally, the emergence of invasive disease by nontoxigenic strains of C. diphtheriae and the reemergence of diphtheria in partially immunized populations have given impetus to new studies in relation to its structural and functional genome. Results: In relation to structural genomics, 23 coding regions (coding sequences) were deleted and 71 new genes were added to the genome annotation. Nevertheless, all the pseudogenes were validated and ten new pseudogenes were created. In relation to functional genomics, about 57% of the genome annotation was updated and became functionally more informative. The product descriptions of 41% (973 proteins) were updated. Among them, 370 that were previously annotated as ?hypothetical proteins,? now have more informative descriptions. With the new annotation, the plasticity of the genome became evident, which shows improvements in the annotation of 13 pathogenicity islands already des... Mostrar Tudo |
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Palavras-Chave: |
Bioinformática. |
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Thesagro: |
Genoma; Patogenicidade. |
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Thesaurus Nal: |
Bioinformatics; Corynebacterium diphtheriae; Genome; Pathogenicity islands. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/70533/1/OAB-25500-re-annotation-of-the-corynebacterium-diphtheriae-nctc13129-g-022412.pdf
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Marc: |
LEADER 03832naa a2200481 a 4500
001 1940203
005 2020-04-14
008 2012 bl uuuu u00u1 u #d
100 1 $aD'AFONSECA, V.
245 $aReannotation of the Corynebacterium diphtheriae NCTC13129 genome as a new approach to studying gene targets connected to virulence and pathogenicity in diphtheria.$h[electronic resource]
260 $c2012
520 $aBackground: The reannotation of genomes already on file is a new approach to discovering new genetic elements and to make the genomes more descriptive and current with relevant features regarding the organism?s lifestyle. Within this approach, the present study aimed to reannotate the genome of the Gram-positive human pathogen Corynebacterium diphtheriae, which causes diphtheria. The deposit of massive amounts of information linked to other species of the genus Corynebacterium has facilitated the updating of the genomic interpretation of this microorganism. Additionally, the emergence of invasive disease by nontoxigenic strains of C. diphtheriae and the reemergence of diphtheria in partially immunized populations have given impetus to new studies in relation to its structural and functional genome. Results: In relation to structural genomics, 23 coding regions (coding sequences) were deleted and 71 new genes were added to the genome annotation. Nevertheless, all the pseudogenes were validated and ten new pseudogenes were created. In relation to functional genomics, about 57% of the genome annotation was updated and became functionally more informative. The product descriptions of 41% (973 proteins) were updated. Among them, 370 that were previously annotated as ?hypothetical proteins,? now have more informative descriptions. With the new annotation, the plasticity of the genome became evident, which shows improvements in the annotation of 13 pathogenicity islands already described in the literature. In addition, the large number of transposases and the presence of structural genes of bacteriophages make their genomic versatility evident. Contrasting with this reality, it also allowed the clarification of some aspects concerned with mechanisms used by C. diphtheriae to stop the invasion of the genome by bacteriophages, mediated by the clustered regularly interspaced short palindromic repeats region. Conclusion: The reannotation of the C. diphtheriae genome provided an improvement in annotation of the C. diphtheriae genome in several aspects, such as virulence characteristics and plasticity events. Moreover, the protocol used here can be extended to various other pathogens in order to improve the genomic information already on file in public databases and to minimize propagating errors. The reannotated archive and updated archive are available at: http://lgcm.icb.ufmg.br/pub/C_diphtheriae_reannotation.embl.
650 $aBioinformatics
650 $aCorynebacterium diphtheriae
650 $aGenome
650 $aPathogenicity islands
650 $aGenoma
650 $aPatogenicidade
653 $aBioinformática
700 1 $aSOARES, S. C.
700 1 $aALI, A.
700 1 $aSANTOS, A. R.
700 1 $aPINTO, A. C.
700 1 $aMAGALHÃES, A. A. C.
700 1 $aFARIA, C. de J.
700 1 $aBARBOSA, E.
700 1 $aGUIMARÃES, L. C.
700 1 $aESLABÃO, M.
700 1 $aALMEIDA, S. S.
700 1 $aABREU, V. A. C.
700 1 $aZERLOTINI, A.
700 1 $aCARNEIRO, A. R.
700 1 $aCERDEIRA, L. T.
700 1 $aRAMOS, R. T. J.
700 1 $aHIRATA JÚNIOR, R.
700 1 $aMATTOS-GUARALDI, A. L.
700 1 $aTROST, E.
700 1 $aTAUCH, A.
700 1 $aSILVA, A.
700 1 $aSCHNEIDER, M. P.
700 1 $aMIYOSHI, A.
700 1 $aAZEVEDO, V.
773 $tOpen Access Bioinformatics$gv. 4, p. 1-13, 2012.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
22/06/2020 |
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Data da última atualização: |
22/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SANTANA, S. R. A.; VOLTOLINI, T. V.; ANTUNES, G. dos R.; SILVA, V. M. da; SIMOES, W. L.; MORGANTE, C. V.; FREITAS, A. D. S. de; CHAVES, A. R. de M.; AIDAR, S. de T.; FERNANDES JUNIOR, P. I. |
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Afiliação: |
Sheilla Rios Assis Santana; TADEU VINHAS VOLTOLINI, CPATSA; Gabiane dos Reis Antunes; Valterlina Moreira da Silva; WELSON LIMA SIMOES, CPATSA; CAROLINA VIANNA MORGANTE, CPATSA; Ana Dolores Santiago de Freitas; AGNALDO RODRIGUES DE MELO CHAVES, CPATSA; SAULO DE TARSO AIDAR, CPATSA; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
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Título: |
Inoculation of plant growth promoting bacteria attenuates the negative efects of drought on sorghum. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Archives of Microbiology, v. 202, p. 1015-1024, 2020. |
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DOI: |
https://doi.org/10.1007/s00203-020-01810-5 |
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Idioma: |
Inglês |
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Conteúdo: |
Microbial inoculants are suitable cost-efective technology to help plants endure drought. For the development of commercial inoculants, screening of efcient plant growth-promoting bacteria (PGPB) is a crucial step. The aim of this study was to evaluate the performance of PGPB to modulate drought resistance in Sorghum bicolor. A pot experiment with sorghum was conducted to access the role of previously selected PGPB strains. In addition, two non-inoculated control treatments (with and without urea fertilization) were also evaluated. For comparison, a fully irrigated treatment (FIT) was also assessed. All plants were fully irrigated for 47 days when the water supply was completely suspended for the drought-stressed treatments. When the soil moisture was close to zero, the irrigation was resumed. During dehydration and rehydration process, the leaf gas exchange (LGE) was evaluated. The parameters of plant growth and nitrogen nutrition were assessed 8 days after reirrigation. Comparing to the FIT, all treatments reduced the LGE rates, but in the presence of Bacillus sp. ESA 402 photosynthesis rate was less reduced. Some inoculation treatments promoted better recovery of photosynthesis, comparable to the FIT, 6 days after rehydration. The plant growth and nitrogen nutrition were negatively afected by the drought, but the inoculation of diferent bacteria reduced some negative efects. The nitrogen accumulation in the shoots was increased by all strains, suggesting their diazotrophic ability even under drought. Overall, the inoculation of Bacillus sp. ESA 402 was the best bacterium with potential for future feld trials. MenosMicrobial inoculants are suitable cost-efective technology to help plants endure drought. For the development of commercial inoculants, screening of efcient plant growth-promoting bacteria (PGPB) is a crucial step. The aim of this study was to evaluate the performance of PGPB to modulate drought resistance in Sorghum bicolor. A pot experiment with sorghum was conducted to access the role of previously selected PGPB strains. In addition, two non-inoculated control treatments (with and without urea fertilization) were also evaluated. For comparison, a fully irrigated treatment (FIT) was also assessed. All plants were fully irrigated for 47 days when the water supply was completely suspended for the drought-stressed treatments. When the soil moisture was close to zero, the irrigation was resumed. During dehydration and rehydration process, the leaf gas exchange (LGE) was evaluated. The parameters of plant growth and nitrogen nutrition were assessed 8 days after reirrigation. Comparing to the FIT, all treatments reduced the LGE rates, but in the presence of Bacillus sp. ESA 402 photosynthesis rate was less reduced. Some inoculation treatments promoted better recovery of photosynthesis, comparable to the FIT, 6 days after rehydration. The plant growth and nitrogen nutrition were negatively afected by the drought, but the inoculation of diferent bacteria reduced some negative efects. The nitrogen accumulation in the shoots was increased by all strains, suggesting their diazotrophi... Mostrar Tudo |
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Palavras-Chave: |
Bactérias diazotróficas; Déficit hídrico; Inoculantes; Semiárido; Trocas de gases foliares. |
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Thesagro: |
Bactéria; Sorgo. |
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Thesaurus NAL: |
Bacillus (bacteria). |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02670naa a2200337 a 4500
001 2123414
005 2020-06-22
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00203-020-01810-5$2DOI
100 1 $aSANTANA, S. R. A.
245 $aInoculation of plant growth promoting bacteria attenuates the negative efects of drought on sorghum.$h[electronic resource]
260 $c2020
520 $aMicrobial inoculants are suitable cost-efective technology to help plants endure drought. For the development of commercial inoculants, screening of efcient plant growth-promoting bacteria (PGPB) is a crucial step. The aim of this study was to evaluate the performance of PGPB to modulate drought resistance in Sorghum bicolor. A pot experiment with sorghum was conducted to access the role of previously selected PGPB strains. In addition, two non-inoculated control treatments (with and without urea fertilization) were also evaluated. For comparison, a fully irrigated treatment (FIT) was also assessed. All plants were fully irrigated for 47 days when the water supply was completely suspended for the drought-stressed treatments. When the soil moisture was close to zero, the irrigation was resumed. During dehydration and rehydration process, the leaf gas exchange (LGE) was evaluated. The parameters of plant growth and nitrogen nutrition were assessed 8 days after reirrigation. Comparing to the FIT, all treatments reduced the LGE rates, but in the presence of Bacillus sp. ESA 402 photosynthesis rate was less reduced. Some inoculation treatments promoted better recovery of photosynthesis, comparable to the FIT, 6 days after rehydration. The plant growth and nitrogen nutrition were negatively afected by the drought, but the inoculation of diferent bacteria reduced some negative efects. The nitrogen accumulation in the shoots was increased by all strains, suggesting their diazotrophic ability even under drought. Overall, the inoculation of Bacillus sp. ESA 402 was the best bacterium with potential for future feld trials.
650 $aBacillus (bacteria)
650 $aBactéria
650 $aSorgo
653 $aBactérias diazotróficas
653 $aDéficit hídrico
653 $aInoculantes
653 $aSemiárido
653 $aTrocas de gases foliares
700 1 $aVOLTOLINI, T. V.
700 1 $aANTUNES, G. dos R.
700 1 $aSILVA, V. M. da
700 1 $aSIMOES, W. L.
700 1 $aMORGANTE, C. V.
700 1 $aFREITAS, A. D. S. de
700 1 $aCHAVES, A. R. de M.
700 1 $aAIDAR, S. de T.
700 1 $aFERNANDES JUNIOR, P. I.
773 $tArchives of Microbiology$gv. 202, p. 1015-1024, 2020.
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