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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
08/08/2011 |
Data da última atualização: |
08/08/2011 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RAMOS, C. A. N.; ARAUJO, F. R.; SOUZA, I. I. F.; BACANELLI, G.; LUIZ, H. L.; RUSSI, L. S.; OLIVEIRA, R. H. M. de; SOARES, C. O.; ROSINHA, G. M. S.; ALVES, L. C. |
Afiliação: |
Carlos Alberto do Nascimento Ramos, Bolsista; FLABIO RIBEIRO ARAUJO, CNPGC; Ingrid I.F. Souza, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco; G. Bacanelli, BOLSISTA; Hera L. Luiz, Bolsista; Lívia S. Russi, Bolsista; RENATO HENRIQUE MARCAL DE OLIVEIRA, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; GRACIA MARIA SOARES ROSINHA, CNPGC; Leucio C. Alves, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco. |
Título: |
Real-time polymerase chain reaction based on msa2c gene for detection of Babesia bovis. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Veterinary Parasitology, v.176, p.79-83, Feb. 2011. Issue 1. |
Idioma: |
Inglês |
Conteúdo: |
This paper reports a quantitative real-time polymerase chain reaction (q-PCR) based on the msa2c gene and standardized with Platinum SYBR Green/ROX for the detection of Babesia bovis in cattle. The msa2c q-PCR amplified a DNA fragment with average dissociation temperature of 77.41 ◦C (±0.25 ◦C). No amplification was detected when DNA from B. bigemina, A. marginale or Bos taurus was used as the template. The detection limit of the msa2c q-PCR was 1000 copies per ml of blood sample, with a linear correlation between the number of msa2c copies and threshold cycle. The comparison between msa2c q-PCR and conventional PCR for cytochrome b revealed 88.8% agreement, with a Kappa index of 0.75. In the comparison between msa2c q-PCR and an enzyme-linked immunosorbent assay (ELISA) with semi-purified B. bovis antigen, agreement was 96.3% and the Kappa index was 0.91. The agreement between three tests was 85.8%. The msa2c q-PCR detected a higher number of positive cattle than conventional PCR in an enzootically stable area, but did not differ significantly from ELISA. No significant differences were detected between the three diagnostic tests with cattle from an enzootically unstable area. All animals raised on a tick-free facility were negative for B. bovis in the three tests. These results suggest that msa2c q-PCR is a useful test for the detection of B. bovis infection. |
Thesagro: |
Babesia Bovis; Babesiose; Sanidade Animal. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02160naa a2200265 a 4500 001 1897432 005 2011-08-08 008 2011 bl uuuu u00u1 u #d 100 1 $aRAMOS, C. A. N. 245 $aReal-time polymerase chain reaction based on msa2c gene for detection of Babesia bovis.$h[electronic resource] 260 $c2011 520 $aThis paper reports a quantitative real-time polymerase chain reaction (q-PCR) based on the msa2c gene and standardized with Platinum SYBR Green/ROX for the detection of Babesia bovis in cattle. The msa2c q-PCR amplified a DNA fragment with average dissociation temperature of 77.41 ◦C (±0.25 ◦C). No amplification was detected when DNA from B. bigemina, A. marginale or Bos taurus was used as the template. The detection limit of the msa2c q-PCR was 1000 copies per ml of blood sample, with a linear correlation between the number of msa2c copies and threshold cycle. The comparison between msa2c q-PCR and conventional PCR for cytochrome b revealed 88.8% agreement, with a Kappa index of 0.75. In the comparison between msa2c q-PCR and an enzyme-linked immunosorbent assay (ELISA) with semi-purified B. bovis antigen, agreement was 96.3% and the Kappa index was 0.91. The agreement between three tests was 85.8%. The msa2c q-PCR detected a higher number of positive cattle than conventional PCR in an enzootically stable area, but did not differ significantly from ELISA. No significant differences were detected between the three diagnostic tests with cattle from an enzootically unstable area. All animals raised on a tick-free facility were negative for B. bovis in the three tests. These results suggest that msa2c q-PCR is a useful test for the detection of B. bovis infection. 650 $aBabesia Bovis 650 $aBabesiose 650 $aSanidade Animal 700 1 $aARAUJO, F. R. 700 1 $aSOUZA, I. I. F. 700 1 $aBACANELLI, G. 700 1 $aLUIZ, H. L. 700 1 $aRUSSI, L. S. 700 1 $aOLIVEIRA, R. H. M. de 700 1 $aSOARES, C. O. 700 1 $aROSINHA, G. M. S. 700 1 $aALVES, L. C. 773 $tVeterinary Parasitology$gv.176, p.79-83, Feb. 2011. Issue 1.
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Embrapa Gado de Corte (CNPGC) |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
13/11/2013 |
Data da última atualização: |
13/11/2013 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SANTANA, J. C. N. de; LUDKE, M. do C. M. M.; LUDKE, J. V.; PEREIRA, P. S.; SILVA, A. S. |
Afiliação: |
JULIANA CLÁUDIA NEVES DE SANTANA, UFRPE; MARIA DO CARMO MOHAUPT MARQUES LUDKE, UFRPE; JORGE VITOR LUDKE, CNPSA; UFRPE; ADIEL SOUZA SILVA, UFRPE. |
Título: |
Nutrition evaluation and metabolizable energy of autoclaved castor bean meal for broilers. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
In: PSA ANNUAL MEETING, 2013, San Diego. Abstracts... Champaign: Poultry Science Association, 2013. p. 140. Publicado no Poultry Science, v. 92, E-Suppl. 1, 2013. |
Idioma: |
Inglês |
Thesagro: |
Frango de corte; Mamona; Metabolismo; Nutrição animal. |
Thesaurus NAL: |
Animal nutrition; Broiler chickens; Castor beans; Feed processing; Metabolism. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/92469/1/final7266.pdf
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Marc: |
LEADER 00865nam a2200253 a 4500 001 1971129 005 2013-11-13 008 2013 bl uuuu u00u1 u #d 100 1 $aSANTANA, J. C. N. de 245 $aNutrition evaluation and metabolizable energy of autoclaved castor bean meal for broilers.$h[electronic resource] 260 $aIn: PSA ANNUAL MEETING, 2013, San Diego. Abstracts... Champaign: Poultry Science Association, 2013. p. 140. Publicado no Poultry Science, v. 92, E-Suppl. 1$c2013 650 $aAnimal nutrition 650 $aBroiler chickens 650 $aCastor beans 650 $aFeed processing 650 $aMetabolism 650 $aFrango de corte 650 $aMamona 650 $aMetabolismo 650 $aNutrição animal 700 1 $aLUDKE, M. do C. M. M. 700 1 $aLUDKE, J. V. 700 1 $aPEREIRA, P. S. 700 1 $aSILVA, A. S.
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