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Registro Completo |
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Biblioteca(s): |
Embrapa Café; Embrapa Unidades Centrais. |
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Data corrente: |
18/02/2011 |
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Data da última atualização: |
07/07/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMIRO, D.; JALLOUL, A.; PETITOT, A-S.; SA, M. F. G. de; MALUF, M. P.; FERNANDEZ, D. |
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Afiliação: |
DANIEL RAMIRO, CIRAD; AIDA JALLOUL, University of Damascus; ANNE-SOPHIE PETITOT, CIRAD; MARIA FATIMA GROSSI DE SA, CENARGEN; MIRIAN PEREZ MALUF, SAPC; DIANA FERNANDEZ, CIRAD. |
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Título: |
Identification of coffee WRKY transcription factor genes and expression in resistance responses to pathogens. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
TREE GENETICS & GENOMES, Curitiba, v.6, p.767-781. 2010. |
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Páginas: |
767-781 |
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Idioma: |
Português |
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Conteúdo: |
In plants, WRKY proteins are a group of transcription factors existing as a gene superfamily that play important roles in regulation of defense response pathways. To assess the diversity of this protein family in coffee (Coffea spp.), data mining methods were used on a set of around 200,000 coffee expressed sequence tags. A total of 53 different putative WRKY genes were obtained, but only 22 unigenes encoding a protein with a WRKY domain were identified, eight of which are supported by full-length cDNA sequences. Alignment of WRKY domain sequences of the coffee unigenes and 72 Arabidopsis thaliana WRKY genes showed that the 22 coffee WRKY members were distributed among the main A. thaliana WRKY subgroups and shared conserved peptide domains. To assess the involvement of WRKY genes in coffee defense response pathways, their expression was analyzed under biotic (nematode and rust fungus infection), hormonal (salicylic acid, methyl-jasmonate), and wounding treatments, leaf senescence, and fruit development. Five members of WRKY groups IId and III were regulated only by pathogens and hormone treatments. Although a significant correlation of WRKY genes expression after MeJA and rust treatments was observed, expression of coffee genes involved in JA biosynthesis and lipoxygenase (EC 1.13.11.12) activity assays did not support the involvement of JA in the early coffee resistance responses to the rust pathogen. The five WRKY transcription factor members identified might play important roles as regulators of pathogen resistance responses and could be useful for improving coffee tolerance to various biotic stresses. MenosIn plants, WRKY proteins are a group of transcription factors existing as a gene superfamily that play important roles in regulation of defense response pathways. To assess the diversity of this protein family in coffee (Coffea spp.), data mining methods were used on a set of around 200,000 coffee expressed sequence tags. A total of 53 different putative WRKY genes were obtained, but only 22 unigenes encoding a protein with a WRKY domain were identified, eight of which are supported by full-length cDNA sequences. Alignment of WRKY domain sequences of the coffee unigenes and 72 Arabidopsis thaliana WRKY genes showed that the 22 coffee WRKY members were distributed among the main A. thaliana WRKY subgroups and shared conserved peptide domains. To assess the involvement of WRKY genes in coffee defense response pathways, their expression was analyzed under biotic (nematode and rust fungus infection), hormonal (salicylic acid, methyl-jasmonate), and wounding treatments, leaf senescence, and fruit development. Five members of WRKY groups IId and III were regulated only by pathogens and hormone treatments. Although a significant correlation of WRKY genes expression after MeJA and rust treatments was observed, expression of coffee genes involved in JA biosynthesis and lipoxygenase (EC 1.13.11.12) activity assays did not support the involvement of JA in the early coffee resistance responses to the rust pathogen. The five WRKY transcription factor members identified might play importa... Mostrar Tudo |
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Palavras-Chave: |
Rust disease. |
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Thesagro: |
Coffea Arábica. |
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Thesaurus Nal: |
Regulator genes. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/29339/1/Identification-of-coffee.pdf
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Marc: |
LEADER 02308naa a2200229 a 4500
001 1880488
005 2011-07-07
008 2010 bl uuuu u00u1 u #d
100 1 $aRAMIRO, D.
245 $aIdentification of coffee WRKY transcription factor genes and expression in resistance responses to pathogens.$h[electronic resource]
260 $c2010
300 $a767-781
520 $aIn plants, WRKY proteins are a group of transcription factors existing as a gene superfamily that play important roles in regulation of defense response pathways. To assess the diversity of this protein family in coffee (Coffea spp.), data mining methods were used on a set of around 200,000 coffee expressed sequence tags. A total of 53 different putative WRKY genes were obtained, but only 22 unigenes encoding a protein with a WRKY domain were identified, eight of which are supported by full-length cDNA sequences. Alignment of WRKY domain sequences of the coffee unigenes and 72 Arabidopsis thaliana WRKY genes showed that the 22 coffee WRKY members were distributed among the main A. thaliana WRKY subgroups and shared conserved peptide domains. To assess the involvement of WRKY genes in coffee defense response pathways, their expression was analyzed under biotic (nematode and rust fungus infection), hormonal (salicylic acid, methyl-jasmonate), and wounding treatments, leaf senescence, and fruit development. Five members of WRKY groups IId and III were regulated only by pathogens and hormone treatments. Although a significant correlation of WRKY genes expression after MeJA and rust treatments was observed, expression of coffee genes involved in JA biosynthesis and lipoxygenase (EC 1.13.11.12) activity assays did not support the involvement of JA in the early coffee resistance responses to the rust pathogen. The five WRKY transcription factor members identified might play important roles as regulators of pathogen resistance responses and could be useful for improving coffee tolerance to various biotic stresses.
650 $aRegulator genes
650 $aCoffea Arábica
653 $aRust disease
700 1 $aJALLOUL, A.
700 1 $aPETITOT, A-S.
700 1 $aSA, M. F. G. de
700 1 $aMALUF, M. P.
700 1 $aFERNANDEZ, D.
773 $tTREE GENETICS & GENOMES, Curitiba$gv.6, p.767-781. 2010.
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 6 | |
| 5. |  | RAMIRO, D. A.; TOMA-BRAGHINI, M.; PETITOT, A. -S.; MALUF, M. P.; FERNANDEZ, D. Use of leaf-disk technique for gene expression analysis of the coffee responses to Hemileia vastatrix Infection. In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 6 | |
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