Data/hora: 06/06/2024 02:47:03

Biblioteca(s): 	Embrapa Arroz e Feijão; Embrapa Mandioca e Fruticultura.	
Data corrente: 	28/10/2004	
Data da última atualização: 	04/11/2004	
Autoria: 	OLIVEIRA, R. P. de; RADMANN, E. B.	
Título: 	Genetic characterization of new varieties and hybrids of citrus table fruit through isoenzymes.	
Ano de publicação: 	2003	
Fonte/Imprenta: 	Crop Breeding and Applied Biotechnology, Londrina, v. 3, n. 1, p. 77-82, Mar. 2003.	
Idioma: 	Inglês	
Conteúdo: 	The aim of this study was to establish isoenzymatic patterns to genetically characterize the orange varieties 'Lane Late', 'Navalete', 'Navelina' and 'Salustiana', mandarin varieties 'Clemenules', 'Marisol' and 'Satsuma Okitsu', and the hybrids 'Nova' and 'Ortanique'. The following isoenzymatic systems were utilized: alcohol dehydrogenase (ADH), esterase (EST), acid phosphatase (APS), phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), 6-phosphogluconate dehydrogenase (6-PGDH), glutamate oxaloacetate transaminase (GOT), isocitrate dehydrogenase (IDH), leucine aminopeptidase (LAP), malate dehydrogenase (MDH), peroxidase (PRX), sorbitol dehydrogenase (SDH), superoxidase dismutase (SOD) and shikimate dehydrogenase (SKDH). Proteins were extracted from leaves and the isoenzymatic polymorphisms were analyzed by polyacrylamide fel electrophoresis. The PGM isoenzyme showed the highest differentiation among the genetic materials tested. The banding profiles observed for PGM, SKDH and EST were satisfactory to characterize the mandarin varieties and hybrids studied. However, no polymorphic bands were found in sweet oranges with the 14 isoenzymes tested.	
Palavras-Chave: 	Biochemical markers; citrus crop; genotype identification; Identification; Orange.	
Thesagro: 	Laranja.	
Thesaurus Nal: 	electrophoresis; genotype.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Arroz e Feijão (CNPAF)	
Exemplares: 	CNPAF - (21801-1) - ADD - AP - -- - 631.05 - C - 2004.00149 - DIS
CNPMF - (22239-1) - ADP - AP - -- -  -  -  - DIS