Data/hora: 02/06/2024 17:29:31

Biblioteca(s): 	Embrapa Agrobiologia.	
Data corrente: 	12/06/2017	
Data da última atualização: 	12/06/2017	
Autoria: 	MATOS, G. F. de; ZILLI, J. E.; ARAUJO, J. L. S. de; LEME, M. M. P.; MELO, I. S. de; RADL, V.; BALDANI, J. I.; ROUWS, L. F. M.	
Afiliação: 	GUSTAVO FEITOSA DE MATOS, UFRRJ; JERRI EDSON ZILLI, CNPAB; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; MARCIA MARIA PARMA LEME, CNPMA; ITAMAR SOARES DE MELO, CNPMA; VIVIANE RADL, HELMHOLTZ ZENTRUM MÜNCHEN, RESEARCH UNIT COMPARATIVE MICROBIOME ANALYSIS; JOSE IVO BALDANI, CNPAB; LUC FELICIANUS MARIE ROUWS, CNPAB.	
Título: 	Bradyrhizobium sacchari sp. nov., a legume nodulating bacterium isolated from sugarcane roots	
Ano de publicação: 	2017	
Fonte/Imprenta: 	Archives of Microbiology, 10 June 2017.	
DOI: 	10.1007/s00203-017-1398-6	
Idioma: 	Inglês	
Conteúdo: 	Members of the genus Bradyrhizobium are well-known as nitrogen-fixing microsymbionts of a wide variety of leguminous species, but they have also been found in different environments, notably as endophytes in non-legumes such as sugarcane. This study presents a detailed polyphasic characterization of four Bradyrhizobium strains (type strain BR 10280T), previously isolated from roots of sugarcane in Brazil. 16S rRNA sequence analysis, multilocus sequence analysis (MLSA) and analysis of the 16S-23S rRNA internal transcribed spacer showed that these strains form a novel clade close to, but different from B. huanghuaihaiense strain CCBAU 23303T. Average nucleotide identity (ANI) analyses confirmed that BR 10280T represents a novel species. Phylogenetic analysis based on nodC gene sequences also placed the strains close to CCBAU 23303T, but different from this latter strain, the sugarcane strains did not nodulate soybean, although they effectively nodulated Vigna unguiculata, Cajanus cajan and Macroptilium atropurpureum. Physiological traits are in agreement with the placement of the strains in the genus Bradyrhizobium as a novel species for which the name Bradyrhizobium sacchari sp. nov. is proposed.	
Palavras-Chave: 	Average nucleotide; Biological nitrogen fixation; Free Living nitrogen fixation; Multilocus sequence analysis; Non Legumes.	
Categoria do assunto: 	S Ciências Biológicas	
Registro original: 	Embrapa Agrobiologia (CNPAB)	
Exemplares: 	CNPAB - (40506-1) - UPC - AP - DD - 2017.00055 -  - 2017.00055 - DIS