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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
31/05/2007 |
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Data da última atualização: |
08/07/2019 |
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Autoria: |
ESTRADA-NAVARRETE, G.; ALVARADO-AFFANTRANGER, X.; OLIVARES, J.-E.; DÍAZ-CAMINO, C.; SANTANA, O.; MURILLO, E.; GUILLÉN, G.; SÁNCHEZ-GUEVARA, N.; ACOSTA, J.; QUINTO, C.; LI, D.; GRESSHOFF, P. M.; SÁNCHEZ, F. |
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Título: |
Agrobacterium rhizogenes transformation of the Phaseolus spp.: a tool for functional genomics. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Molecular Plant-Microbe Interactions, St. Paul, v. 19, n. 12, p. 1385-1393, 2006. |
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Idioma: |
Inglês |
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Conteúdo: |
A fast, reproducible, and efficient transformation procedure employing Agrobacterium rhizogenes was developed for Phaseolus vulgaris L. wild accessions, land races, and culti-vars and for three other species belonging to the genus Phaseolus: P. coccineus, P. lunatus, and P. acutifolius. In-duced hairy roots are robust and grow quickly. The trans-formation frequency is between 75 and 90% based on the 35.S promoter-driven green fluorescent protein and f)-glu-curonidase expression reporter constructs. When inoculated with Rhizobium tropici, transgenic roots induce normal de-terminate nodules that fix nitrogen as etliciently as inocu-lated standard roots. The A. rhizogenes-induced hairy root transformation in the genus Phaseolus sets the foundation for functional genomics programs focused on root physiol-ogy, root metabolism, and root-microbe interactions. |
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Thesaurus Nal: |
Agrobacterium. |
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Categoria do assunto: |
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Marc: |
LEADER 01670naa a2200277 a 4500
001 1629317
005 2019-07-08
008 2006 bl --- 0-- u #d
100 1 $aESTRADA-NAVARRETE, G.
245 $aAgrobacterium rhizogenes transformation of the Phaseolus spp.$ba tool for functional genomics.
260 $c2006
520 $aA fast, reproducible, and efficient transformation procedure employing Agrobacterium rhizogenes was developed for Phaseolus vulgaris L. wild accessions, land races, and culti-vars and for three other species belonging to the genus Phaseolus: P. coccineus, P. lunatus, and P. acutifolius. In-duced hairy roots are robust and grow quickly. The trans-formation frequency is between 75 and 90% based on the 35.S promoter-driven green fluorescent protein and f)-glu-curonidase expression reporter constructs. When inoculated with Rhizobium tropici, transgenic roots induce normal de-terminate nodules that fix nitrogen as etliciently as inocu-lated standard roots. The A. rhizogenes-induced hairy root transformation in the genus Phaseolus sets the foundation for functional genomics programs focused on root physiol-ogy, root metabolism, and root-microbe interactions.
650 $aAgrobacterium
700 1 $aALVARADO-AFFANTRANGER, X.
700 1 $aOLIVARES, J.-E.
700 1 $aDÍAZ-CAMINO, C.
700 1 $aSANTANA, O.
700 1 $aMURILLO, E.
700 1 $aGUILLÉN, G.
700 1 $aSÁNCHEZ-GUEVARA, N.
700 1 $aACOSTA, J.
700 1 $aQUINTO, C.
700 1 $aLI, D.
700 1 $aGRESSHOFF, P. M.
700 1 $aSÁNCHEZ, F.
773 $tMolecular Plant-Microbe Interactions, St. Paul$gv. 19, n. 12, p. 1385-1393, 2006.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| 1. |  | ESTRADA-NAVARRETE, G.; ALVARADO-AFFANTRANGER, X.; OLIVARES, J.-E.; DÍAZ-CAMINO, C.; SANTANA, O.; MURILLO, E.; GUILLÉN, G.; SÁNCHEZ-GUEVARA, N.; ACOSTA, J.; QUINTO, C.; LI, D.; GRESSHOFF, P. M.; SÁNCHEZ, F. Agrobacterium rhizogenes transformation of the Phaseolus spp.: a tool for functional genomics. Molecular Plant-Microbe Interactions, St. Paul, v. 19, n. 12, p. 1385-1393, 2006.| Biblioteca(s): Embrapa Agrobiologia. |
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