Data/hora: 12/06/2024 19:32:55

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 09/05/2023
Data da última atualização: | 22/08/2023
Autoria: | OLIVEIRA, C. S.; SILVA, M. V. G. B.; QUINTAO, C. C. R.; OTTO, P. I.; ALONSO, R. V.; FERES, L. F.; PANETTO, J. C. do C.; MACHADO, M. A.; CAMARGO, L. S. de A.
Afiliação: | CLARA SLADE OLIVEIRA, CNPGL; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; PAMELA ITAJARA OTTO, Universidade Federal de Santa Maria; RODRIGO VITORIO ALONSO, Nelore Myo Genetica Bovina; LUIZ FERNANDO FERES, Universidade Jose do Rosario Vellano-UNIFENAS; JOAO CLAUDIO DO CARMO PANETTO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL.
Título: | Imputation accuracy for genomic selection using embryo biopsy samples in Gir.
Ano de publicação: | 2023
Fonte/Imprenta: | Reproductive Biology, v. 23, 100765, 2023.
DOI: | https://doi.org/10.1016/j.repbio.2023.100765
Idioma: | Inglês
Conteúdo: | The aim of this study was to establish a platform for genomic selection of in vitro-fertilized (IVF) Gir embryos. Multiple displacement amplification (MDA)-based embryo biopsy samples were genotyped, and genomic estimated breeding values (GEBV) for milk yield (305MY) were calculated. The concordance of GEBV and accuracy between embryo biopsies and the respective liveborn were assessed. Imputation was performed using two panels (Z-Chip and Bovine HD, Illumina) based on a database of 73,110 lactating cow's database and pedigree files from 147,131 animals. Biopsied embryos had similar pregnancy rates (39% vs 40%), pregnancy loss rates (18% vs 20%), and pregnancy length compared to Control embryos. After genotyping, low call rate means were detected for biopsy samples compared to the respective calf samples (0.80 vs 0.98). Imputation presented 0.83 (Z-Chip) and 0.96 (HD) accuracy (CORRanim). Embryo GEBV accuracy levels were higher in BovineHD imputation (0.82) than Z-Chip imputation (0.55) or no imputation (0.62), and the correlation between embryo/calf pairs' accuracy was 0.85 for BovineHD imputation, 0.11 for Z-Chip imputation, and 0.02 for no imputation. GEVB estimates correlation between embryo/calf pairs was 0.87 for BovineHD imputation, 0.80 for Z-Chip imputation, and 0.41 before imputation. The call rate of embryo samples did not affect the correlation between embryo/calf pairs for accuracy and GEBV before and after BovineHD imputation. Embryos obtained on the same farm presented GEBV 305MY differences of up to 800 kg, emphasizing the expected impact of embryo genomic selection for the Gir breed.
Palavras-Chave: | Genotipagem.
Thesagro: | Biopsia; Bovino; Embrião Animal; Gado Gir.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (26008-1) - UPC - AP - DD -  -  -  - DIS