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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
11/04/2018 |
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Data da última atualização: |
14/05/2018 |
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Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
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Autoria: |
QUEIROZ, S. C. do N. de; SOUZA, D. R. C. de; CERDEIRA, A. L.; PARAIBA, L. C. |
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Afiliação: |
SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; DEBORA RENATA CASSOLI DE S DUTRA, CNPMA; ANTONIO LUIZ CERDEIRA, CNPMA; LOURIVAL COSTA PARAIBA, CNPMA. |
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Título: |
Validação de um método para determinação de resíduos de fungicidas em grãos de soja. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Jaguariúna: Embrapa Meio Ambiente, 2017. |
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Páginas: |
19 p. |
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Série: |
(Embrapa Meio Ambiente. Boletim de Pesquisa e Desenvolvimento, 73). |
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ISSN: |
1516-4675 |
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Idioma: |
Português |
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Conteúdo: |
O cultivo de soja utiliza diversos tipos de fungicidas para controlar fungos fitopatogênicos, que, dependendo das características físico-químicas das moléculas, podem se acumular nos grãos e causar riscos à saúde humana. Desse modo, para verificar a presença de resíduos destas substâncias nesta matriz alimentar há necessidade de desenvolver e validar métodos analíticos que sejam sensíveis e confiáveis. Assim, o objetivo deste trabalho foi adaptar e validar um método para a determinação dos fungicidas não-iônicos ciproconazol, epoxiconazol, piraclostrobina e azoxistrobina em grãos de soja, para ser aplicado na validação de um modelo matemático cinético de bioconcentração, a fim de prever o acúmulo desses agrotóxicos nesse alimento. O método analítico baseou-se na abordagem QuEChERS (do inglês Quick, Easy, Cheap, Effective, Rugged and Safe) - acetato para o preparo de amostra e para a quantificação e confirmação da técnica de cromatografia gasosa acoplada à espectrometria de massas (GC-MS/MS). Os parâmetros de validação avaliados foram: linearidade maior ou igual a 0,99, exatidão (em termos % de recuperação) de 70-120 % e precisão menor ou igual a 20 % (em termos de repetitividade na forma de coeficiente de variação-CV%), limites de quantificação (LQ) iguais a 10 micrograma kg-1 para ciproconazol, 5 micrograma kg-1 para epoxiconazol e azoxistrobina, e 100 micrograma kg-1para piraclostrobina. Portanto, o método foi adequado para o uso pretendido. //// Phytopathogenic diseases in soybean are controlled with several types of fungicides, which, depending on the physico-chemical characteristics of the molecules, they can accumulate in grains and cause risks to human health. Thus, to verify the presence of residues of these substances there is a need to develop and validate analytical methods that are sensitive and reliable. The objective of this study was to adapt and validate a method for the determination of non-ionic fungicides such as cyproconazole, epoxiconazole, pyraclostrobin and azoxystrobin in soybeans, to be used to validate a model of kinetic bioconcentration in order to predict the accumulation of the pesticides in this food. The analytical method was based on QuEChERS approach (English Quick, Easy, Cheap, Effective, Rugged and Safe)-acetate for sample preparation, quantification and confirmation with gas chromatography coupled to mass spectrometry (GC-MS/MS). The validation parameters evaluated were: linearity greater than or equal to 0.99, accuracy (in terms of % recovery) of 70-120% and precision less than or equal to 20% (in terms of repeatability as coefficient of variation), quantification limits (QL) equal to 10 microgram kg-1 for cyproconazole, 5 microgram kg-1 for epoxiconazole and azoxystrobin and 100 micrograma kg-1 for pyraclostrobin. Thus, the method was suitable for the intended use. MenosO cultivo de soja utiliza diversos tipos de fungicidas para controlar fungos fitopatogênicos, que, dependendo das características físico-químicas das moléculas, podem se acumular nos grãos e causar riscos à saúde humana. Desse modo, para verificar a presença de resíduos destas substâncias nesta matriz alimentar há necessidade de desenvolver e validar métodos analíticos que sejam sensíveis e confiáveis. Assim, o objetivo deste trabalho foi adaptar e validar um método para a determinação dos fungicidas não-iônicos ciproconazol, epoxiconazol, piraclostrobina e azoxistrobina em grãos de soja, para ser aplicado na validação de um modelo matemático cinético de bioconcentração, a fim de prever o acúmulo desses agrotóxicos nesse alimento. O método analítico baseou-se na abordagem QuEChERS (do inglês Quick, Easy, Cheap, Effective, Rugged and Safe) - acetato para o preparo de amostra e para a quantificação e confirmação da técnica de cromatografia gasosa acoplada à espectrometria de massas (GC-MS/MS). Os parâmetros de validação avaliados foram: linearidade maior ou igual a 0,99, exatidão (em termos % de recuperação) de 70-120 % e precisão menor ou igual a 20 % (em termos de repetitividade na forma de coeficiente de variação-CV%), limites de quantificação (LQ) iguais a 10 micrograma kg-1 para ciproconazol, 5 micrograma kg-1 para epoxiconazol e azoxistrobina, e 100 micrograma kg-1para piraclostrobina. Portanto, o método foi adequado para o uso pretendido. //// Phytopathogenic diseases i... Mostrar Tudo |
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Palavras-Chave: |
Mass spectroscopy; Soybean. |
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Thesagro: |
Análise química; Cromatografia gasosa; Fungicida; Resíduo químico; Soja. |
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Thesaurus Nal: |
Chemical analysis; Chemical residues; Chromatography; Fungicides. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/175429/1/2017BP04.pdf
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Marc: |
LEADER 03837nam a2200313 a 4500
001 2090496
005 2018-05-14
008 2017 bl uuuu u0uu1 u #d
022 $a1516-4675
100 1 $aQUEIROZ, S. C. do N. de
245 $aValidação de um método para determinação de resíduos de fungicidas em grãos de soja.$h[electronic resource]
260 $aJaguariúna: Embrapa Meio Ambiente$c2017
300 $a19 p.
490 $a(Embrapa Meio Ambiente. Boletim de Pesquisa e Desenvolvimento, 73).
520 $aO cultivo de soja utiliza diversos tipos de fungicidas para controlar fungos fitopatogênicos, que, dependendo das características físico-químicas das moléculas, podem se acumular nos grãos e causar riscos à saúde humana. Desse modo, para verificar a presença de resíduos destas substâncias nesta matriz alimentar há necessidade de desenvolver e validar métodos analíticos que sejam sensíveis e confiáveis. Assim, o objetivo deste trabalho foi adaptar e validar um método para a determinação dos fungicidas não-iônicos ciproconazol, epoxiconazol, piraclostrobina e azoxistrobina em grãos de soja, para ser aplicado na validação de um modelo matemático cinético de bioconcentração, a fim de prever o acúmulo desses agrotóxicos nesse alimento. O método analítico baseou-se na abordagem QuEChERS (do inglês Quick, Easy, Cheap, Effective, Rugged and Safe) - acetato para o preparo de amostra e para a quantificação e confirmação da técnica de cromatografia gasosa acoplada à espectrometria de massas (GC-MS/MS). Os parâmetros de validação avaliados foram: linearidade maior ou igual a 0,99, exatidão (em termos % de recuperação) de 70-120 % e precisão menor ou igual a 20 % (em termos de repetitividade na forma de coeficiente de variação-CV%), limites de quantificação (LQ) iguais a 10 micrograma kg-1 para ciproconazol, 5 micrograma kg-1 para epoxiconazol e azoxistrobina, e 100 micrograma kg-1para piraclostrobina. Portanto, o método foi adequado para o uso pretendido. //// Phytopathogenic diseases in soybean are controlled with several types of fungicides, which, depending on the physico-chemical characteristics of the molecules, they can accumulate in grains and cause risks to human health. Thus, to verify the presence of residues of these substances there is a need to develop and validate analytical methods that are sensitive and reliable. The objective of this study was to adapt and validate a method for the determination of non-ionic fungicides such as cyproconazole, epoxiconazole, pyraclostrobin and azoxystrobin in soybeans, to be used to validate a model of kinetic bioconcentration in order to predict the accumulation of the pesticides in this food. The analytical method was based on QuEChERS approach (English Quick, Easy, Cheap, Effective, Rugged and Safe)-acetate for sample preparation, quantification and confirmation with gas chromatography coupled to mass spectrometry (GC-MS/MS). The validation parameters evaluated were: linearity greater than or equal to 0.99, accuracy (in terms of % recovery) of 70-120% and precision less than or equal to 20% (in terms of repeatability as coefficient of variation), quantification limits (QL) equal to 10 microgram kg-1 for cyproconazole, 5 microgram kg-1 for epoxiconazole and azoxystrobin and 100 micrograma kg-1 for pyraclostrobin. Thus, the method was suitable for the intended use.
650 $aChemical analysis
650 $aChemical residues
650 $aChromatography
650 $aFungicides
650 $aAnálise química
650 $aCromatografia gasosa
650 $aFungicida
650 $aResíduo químico
650 $aSoja
653 $aMass spectroscopy
653 $aSoybean
700 1 $aSOUZA, D. R. C. de
700 1 $aCERDEIRA, A. L.
700 1 $aPARAIBA, L. C.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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Data corrente: |
12/09/2018 |
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Data da última atualização: |
11/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MAIA, A. L. R. S.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G.; VEIGA, M. O.; BALARO, M. F. A.; SIQUEIRA, L. G. B.; FACO, O.; FONSECA, J. F. da. |
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Afiliação: |
ANA LUCIA R. S. MAIA, Fluminense Federal University; Universidade Federal de Lavras; MARIA JOANNA G. SOUZA-FABJAN, Fluminense Federal University; Universidade Federal de Lavras; Fluminense Federal University; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; OLIVARDO FACO, CNPC; JEFERSON FERREIRA DA FONSECA, CNPC. |
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Título: |
Hydrometra in dairy goats: ultrasonic variables and therapeutic protocols evaluated during the reproductive season. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 197, p. 203-211, 2018. |
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DOI: |
https://doi.org/10.1016/j.anireprosci.2018.08.030 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Hydrometra is characterized by the accumulation of fluid within the uterus due to the persistence of corpus luteum. The diagnosis of this disorder occurs with an ultrasonic exam. This study evaluated uterine drainage and fertility rates in goats after the use of d-cloprostenol in association or not with Gonadotropin-releasing hormone (GnRH) treatment. Twenty Saanen goats, diagnosed with hydrometra, received three 37.5-μg doses of d-cloprostenol laterovulvarly at 10-day intervals. On D5, the goats were assigned into two groups receiving 1 mL of GnRH or saline solution intramuscularly. Ultrasonography (US) was performed from D0 to D25. An US approach was used to rank hydrometra in scores. The pregnancy rate was assessed 45 and 90 days after the end of treatment. The uterine fluid was totally drained after the first and second administration of d-cloprostenol in 50% and 95% of the goats, respectively. In one female, full emptying of the uterus occurred only after D20. US performed at 45 and 90 days after the end of treatment indicated there was a pregnancy rate of 45.0% and 55.0%, respectively. Fertility did not differ between the GnRH-treated and control goats. Those goats not pregnant at 45 days had a follicular cyst, hydrosalpinx or hydrometra. At 90 days, no change was observed in the hydrosalpinx, and four goats had hydrometra. The use of three doses of d-cloprostenol 10 days apart was efficient for induction of draining the contents of the uterus, resulting in a relatively acceptable pregnancy rate. This treatment associated with the US approach can be important when applied in the field. MenosAbstract: Hydrometra is characterized by the accumulation of fluid within the uterus due to the persistence of corpus luteum. The diagnosis of this disorder occurs with an ultrasonic exam. This study evaluated uterine drainage and fertility rates in goats after the use of d-cloprostenol in association or not with Gonadotropin-releasing hormone (GnRH) treatment. Twenty Saanen goats, diagnosed with hydrometra, received three 37.5-μg doses of d-cloprostenol laterovulvarly at 10-day intervals. On D5, the goats were assigned into two groups receiving 1 mL of GnRH or saline solution intramuscularly. Ultrasonography (US) was performed from D0 to D25. An US approach was used to rank hydrometra in scores. The pregnancy rate was assessed 45 and 90 days after the end of treatment. The uterine fluid was totally drained after the first and second administration of d-cloprostenol in 50% and 95% of the goats, respectively. In one female, full emptying of the uterus occurred only after D20. US performed at 45 and 90 days after the end of treatment indicated there was a pregnancy rate of 45.0% and 55.0%, respectively. Fertility did not differ between the GnRH-treated and control goats. Those goats not pregnant at 45 days had a follicular cyst, hydrosalpinx or hydrometra. At 90 days, no change was observed in the hydrosalpinx, and four goats had hydrometra. The use of three doses of d-cloprostenol 10 days apart was efficient for induction of draining the contents of the uterus, resulting... Mostrar Tudo |
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Palavras-Chave: |
Distúrbio reprodutivo; Gestational loss; GnRH; Hidrometra; PGF 2 alpha. |
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Thesagro: |
Cabra Leiteira; Caprino; Distúrbio Animal; Doença Animal. |
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Thesaurus NAL: |
Dairy goats; Female fertility; Female genital diseases; Ovarian diseases; Pregnancy complications; Pregnancy diagnosis; Pregnancy rate; Pseudopregnancy; Reproductive disorders; Uterine diseases. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02964naa a2200445 a 4500
001 2098457
005 2022-08-11
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.anireprosci.2018.08.030$2DOI
100 1 $aMAIA, A. L. R. S.
245 $aHydrometra in dairy goats$bultrasonic variables and therapeutic protocols evaluated during the reproductive season.$h[electronic resource]
260 $c2018
520 $aAbstract: Hydrometra is characterized by the accumulation of fluid within the uterus due to the persistence of corpus luteum. The diagnosis of this disorder occurs with an ultrasonic exam. This study evaluated uterine drainage and fertility rates in goats after the use of d-cloprostenol in association or not with Gonadotropin-releasing hormone (GnRH) treatment. Twenty Saanen goats, diagnosed with hydrometra, received three 37.5-μg doses of d-cloprostenol laterovulvarly at 10-day intervals. On D5, the goats were assigned into two groups receiving 1 mL of GnRH or saline solution intramuscularly. Ultrasonography (US) was performed from D0 to D25. An US approach was used to rank hydrometra in scores. The pregnancy rate was assessed 45 and 90 days after the end of treatment. The uterine fluid was totally drained after the first and second administration of d-cloprostenol in 50% and 95% of the goats, respectively. In one female, full emptying of the uterus occurred only after D20. US performed at 45 and 90 days after the end of treatment indicated there was a pregnancy rate of 45.0% and 55.0%, respectively. Fertility did not differ between the GnRH-treated and control goats. Those goats not pregnant at 45 days had a follicular cyst, hydrosalpinx or hydrometra. At 90 days, no change was observed in the hydrosalpinx, and four goats had hydrometra. The use of three doses of d-cloprostenol 10 days apart was efficient for induction of draining the contents of the uterus, resulting in a relatively acceptable pregnancy rate. This treatment associated with the US approach can be important when applied in the field.
650 $aDairy goats
650 $aFemale fertility
650 $aFemale genital diseases
650 $aOvarian diseases
650 $aPregnancy complications
650 $aPregnancy diagnosis
650 $aPregnancy rate
650 $aPseudopregnancy
650 $aReproductive disorders
650 $aUterine diseases
650 $aCabra Leiteira
650 $aCaprino
650 $aDistúrbio Animal
650 $aDoença Animal
653 $aDistúrbio reprodutivo
653 $aGestational loss
653 $aGnRH
653 $aHidrometra
653 $aPGF 2 alpha
700 1 $aBRANDÃO, F. Z.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aVEIGA, M. O.
700 1 $aBALARO, M. F. A.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aFACO, O.
700 1 $aFONSECA, J. F. da
773 $tAnimal Reproduction Science$gv. 197, p. 203-211, 2018.
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