Data/hora: 15/06/2024 05:16:22

Biblioteca(s): | Embrapa Meio Ambiente.
Data corrente: | 03/12/2019
Data da última atualização: | 08/04/2021
Autoria: | SHIROMA, L. S.; QUEIROZ, S. C. do N. de; JONSSON, C. M.; BOTTOLI, C. B. G.
Afiliação: | LETÍCIA SAYURI SHIROMA, IQ-UNICAMP; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; CLAUDIO MARTIN JONSSON, CNPMA; CARLA BEATRIZ GRESPAN BOTTOLI, IQ-UNICAMP.
Título: | Extraction strategies for simultaneous determination of florfenicol and florfenicol amine in tilapia (Oreochromis niloticus) muscle: quantification by LC-MS/MS.
Ano de publicação: | 2020
Fonte/Imprenta: | Food Analytical Methods, v. 13, n. 1, p. 291-302, 2020.
ISSN: | 1936-976X
DOI: | https://doi.org/10.1007/s12161-019-01633-1
Idioma: | Inglês
Conteúdo: | Abstract: A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of the veterinary drug florfenicol (FF) and its major metabolite, florfenicol amine (FFA), in tilapia muscles (Oreochromis niloticus). Three different sample preparation procedures (DLLME, sub-zero, and modified QuEChERS) were tested. The best extraction results were obtained by using the modified QuEChERS. The quantification was made by using a LC-MS/MS analysis, with a Lichrocart Cartridge Purospher Star C8 HPLC column (250 mm×4.6 mm, 5 µm particle size). Analytes were separated with a mobile phase consisting of Milli-Q water to acetonitrile 40:60 (v/v), both with 0.1% formic acid. The validation parameters were recovery of 70 to 79% and 62 to 69%, limit of detection of 0.0625 µg g-1 and 0.125 µg g-1, and limit of quantification of 0.125 µg g-1 and 0.25 µg g-1, for FF and FFA, respectively. CC alfa was 1183 µg kg-1 and CC beta was 1365 µg kg-1 for FF, intraday and interday precision has CV greater than or equal to 20%, and linear range was 0.625 to 5.00 µg g-1. This method was shown to be simple and rapid when compared to other, more conventional methods. Also, it has low reagent and solvent consumption, with low waste generation, which is in line with the principles of green chemistry. The method was successfully applied for the analyzes of tilapia exposed to the antibiotic.
Palavras-Chave: | Química verde.
Thesagro: | Análise Química; Antibiótico; Cromatografia; Resíduo Quimico; Tilápia.
Thesaurus Nal: | Antibiotic residues; Aquaculture; green chemistry; Liquid chromatography; Oreochromis; Tandem mass spectrometry; Tilapia (common name); Veterinary drugs.
Categoria do assunto: | W Química e Física
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (16557-1) - UPC - AP - DD -  -  -  - DIS