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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria Tropical; Embrapa Semiárido. |
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Data corrente: |
27/05/2022 |
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Data da última atualização: |
27/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AMORIM, C. C. de; SILVA, F. D. da; BORDALLO, P. do N.; ARAGAO, F. A. S. de; QUEIRÓZ, M. A. de. |
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Afiliação: |
CLISNEIDE COELHO DE AMORIM, Programa de Pós-Graduação em Recursos Genéticos Vegetais, Universidade Estadual de Feira de Santana (UEFS); FRANCISCO DAVI DA SILVA, Universidade Federal de Viçosa (UFV); PATRICIA DO NASCIMENTO BORDALLO, CNPAT; FERNANDO ANTONIO SOUZA DE ARAGAO, CNPAT; MANOEL ABÍLIO DE QUEIRÓZ, Universidade do Estado da Bahia (UNEB), Campus Juazeiro-BA. |
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Título: |
Genetic divergence between sub-accessions of the melon using molecular markers. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Revista Ciência Agronômica, v. 53, e20218007, 2022. |
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DOI: |
DOI: 10.5935/1806-6690.20220027 |
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Idioma: |
Inglês |
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Conteúdo: |
The melon (Cucumis melo L.) is one of the most important horticultural crops in the northeast of Brazil. The characterisation of germplasm is an ef ficient process for managing plant genetic resources, as it contributes to better conservation and improved use in breeding programs. In the present study, the genetic divergence between sub-accessions of the melon taken from traditional agriculture in the semi-arid region of Brazil was evaluated using ISSR and RAPD markers, verifying the level of agreement with the botanical classification. Twenty-six sub-accessions preserved in the Active Germplasm Bank of Cucurbitaceae for the northeast of Brazil, located at Embrapa Semiárido in Petrolina, Pernambuco, were analysed. The binary matrix of the molecular data was used to obtain the genetic dissimilarities using the complement of the Jaccard index. The genetic dissimilarities were represented in dendrograms generated using the UPGMA method. Twenty-eight ISSR primers and 26 RAPD primers were polymorphic. A total of 686 fragments were obtained, of which 451 were polymorphic. The mean polymorphism of the RAPD markers was greater than that of the ISSR markers. The number of markers was considered suf ficient to accurately determine the genetic diversity. The ISSR dendrogram allowed the formation of three groups, the RAPD dendrogram, four groups, and the joint dendrogram, three groups. The study demonstrated the genetic variability between the sub-accessions, and the strong association between the characterisation made by the molecular markers and the taxonomic classification of the genotypes under study. MenosThe melon (Cucumis melo L.) is one of the most important horticultural crops in the northeast of Brazil. The characterisation of germplasm is an ef ficient process for managing plant genetic resources, as it contributes to better conservation and improved use in breeding programs. In the present study, the genetic divergence between sub-accessions of the melon taken from traditional agriculture in the semi-arid region of Brazil was evaluated using ISSR and RAPD markers, verifying the level of agreement with the botanical classification. Twenty-six sub-accessions preserved in the Active Germplasm Bank of Cucurbitaceae for the northeast of Brazil, located at Embrapa Semiárido in Petrolina, Pernambuco, were analysed. The binary matrix of the molecular data was used to obtain the genetic dissimilarities using the complement of the Jaccard index. The genetic dissimilarities were represented in dendrograms generated using the UPGMA method. Twenty-eight ISSR primers and 26 RAPD primers were polymorphic. A total of 686 fragments were obtained, of which 451 were polymorphic. The mean polymorphism of the RAPD markers was greater than that of the ISSR markers. The number of markers was considered suf ficient to accurately determine the genetic diversity. The ISSR dendrogram allowed the formation of three groups, the RAPD dendrogram, four groups, and the joint dendrogram, three groups. The study demonstrated the genetic variability between the sub-accessions, and the strong association ... Mostrar Tudo |
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Palavras-Chave: |
Análise de agrupamento; Caracterização molecular; Diversidade genética; Molecular characterisation. |
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Thesagro: |
Biodiversidade; Cucumis Melo; Hortaliça; Melão; Melhoramento Genético Vegetal. |
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Thesaurus Nal: |
Biodiversity; Cluster analysis; Melons. |
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Categoria do assunto: |
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G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1143502/1/ART22047.pdf
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Marc: |
LEADER 02600naa a2200325 a 4500
001 2143502
005 2022-05-27
008 2022 bl uuuu u00u1 u #d
024 7 $aDOI: 10.5935/1806-6690.20220027$2DOI
100 1 $aAMORIM, C. C. de
245 $aGenetic divergence between sub-accessions of the melon using molecular markers.$h[electronic resource]
260 $c2022
520 $aThe melon (Cucumis melo L.) is one of the most important horticultural crops in the northeast of Brazil. The characterisation of germplasm is an ef ficient process for managing plant genetic resources, as it contributes to better conservation and improved use in breeding programs. In the present study, the genetic divergence between sub-accessions of the melon taken from traditional agriculture in the semi-arid region of Brazil was evaluated using ISSR and RAPD markers, verifying the level of agreement with the botanical classification. Twenty-six sub-accessions preserved in the Active Germplasm Bank of Cucurbitaceae for the northeast of Brazil, located at Embrapa Semiárido in Petrolina, Pernambuco, were analysed. The binary matrix of the molecular data was used to obtain the genetic dissimilarities using the complement of the Jaccard index. The genetic dissimilarities were represented in dendrograms generated using the UPGMA method. Twenty-eight ISSR primers and 26 RAPD primers were polymorphic. A total of 686 fragments were obtained, of which 451 were polymorphic. The mean polymorphism of the RAPD markers was greater than that of the ISSR markers. The number of markers was considered suf ficient to accurately determine the genetic diversity. The ISSR dendrogram allowed the formation of three groups, the RAPD dendrogram, four groups, and the joint dendrogram, three groups. The study demonstrated the genetic variability between the sub-accessions, and the strong association between the characterisation made by the molecular markers and the taxonomic classification of the genotypes under study.
650 $aBiodiversity
650 $aCluster analysis
650 $aMelons
650 $aBiodiversidade
650 $aCucumis Melo
650 $aHortaliça
650 $aMelão
650 $aMelhoramento Genético Vegetal
653 $aAnálise de agrupamento
653 $aCaracterização molecular
653 $aDiversidade genética
653 $aMolecular characterisation
700 1 $aSILVA, F. D. da
700 1 $aBORDALLO, P. do N.
700 1 $aARAGAO, F. A. S. de
700 1 $aQUEIRÓZ, M. A. de
773 $tRevista Ciência Agronômica$gv. 53, e20218007, 2022.
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Registro original: |
Embrapa Agroindústria Tropical (CNPAT) |
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| Registros recuperados : 404 | |
| 13. |  | QUEIROZ, M. A. de. Pre-melhoramento em cucurbitaceas. In: SIMPOSIO DE RECURSOS GENETICOS PARA AMERICA LATINA E CARIBE, 2.,1999, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Geneticos e Biotecnologia, 1999. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 15. | | QUEIROZ, M. A. de. Os recursos geneticos vegetais e os melhoristas de plantas. In: QUEIROZ, M. A. de; GOEDERT, C. O.; RAMOS, S. R. R. (Ed.). Recursos genéticos e melhoramento de plantas para o Nordeste brasileiro. Petrolina: Embrapa Semi-Árido; Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 1999. Np.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 404 | |
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