| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
15/04/2004 |
|
Data da última atualização: |
15/04/2004 |
|
Autoria: |
LEGRIS, G.; POTRYKUS, I.; PUONTI-KAERLAS, J. |
|
Título: |
Optimising conditions for biolistic transformation of cassava. |
|
Ano de publicação: |
1998 |
|
Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v. 17, p. 31, nov., 1998. Suplemento. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: Particle bombardment offers a tissue- and genotype-independente transformation technique that can be used to circumvent host strain restrictions sometimes encountered when using Agrobacterium-based transformation methods. We have previously developed an Agrobacterium-based transformation system for cassava. Now we present a novel protocol for Particle Inflow Gun (PIG) - mediated transformation, developed for use in combination with the organogenesis-based shoot regeneration system from somatic cotyledons. The parameters for transformation conditions have been partially optimised using transient GUS assays. The in vitro performance of different cassava cultivars after particle bombardment has been evaluated, and the frequencies of plant regeneration after bombardment and selection in cassava have been significantly improved by optimising the protocols further. The parameters partially optimised so far include the amount of DNA used, the flying distance of the microprojectiles, the duration of pre-and postplasmolysis treatments and the composition of the regeneration medium used after bombardment. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01554naa a2200145 a 4500
001 1651901
005 2004-04-15
008 1998 bl uuuu u00u1 u #d
100 1 $aLEGRIS, G.
245 $aOptimising conditions for biolistic transformation of cassava.
260 $c1998
520 $aAbstract: Particle bombardment offers a tissue- and genotype-independente transformation technique that can be used to circumvent host strain restrictions sometimes encountered when using Agrobacterium-based transformation methods. We have previously developed an Agrobacterium-based transformation system for cassava. Now we present a novel protocol for Particle Inflow Gun (PIG) - mediated transformation, developed for use in combination with the organogenesis-based shoot regeneration system from somatic cotyledons. The parameters for transformation conditions have been partially optimised using transient GUS assays. The in vitro performance of different cassava cultivars after particle bombardment has been evaluated, and the frequencies of plant regeneration after bombardment and selection in cassava have been significantly improved by optimising the protocols further. The parameters partially optimised so far include the amount of DNA used, the flying distance of the microprojectiles, the duration of pre-and postplasmolysis treatments and the composition of the regeneration medium used after bombardment.
700 1 $aPOTRYKUS, I.
700 1 $aPUONTI-KAERLAS, J.
773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv. 17, p. 31, nov., 1998. Suplemento.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Solos. |
|
Data corrente: |
25/11/2019 |
|
Data da última atualização: |
03/01/2020 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
CAMPOS, D. V. B. de; CHAVES, L. da S.; MATTOS, B. B.; BALIEIRO, F. de C. |
|
Afiliação: |
DAVID VILAS BOAS DE CAMPOS, CNPS; LÍVIA DA SILVA CHAVES, UNIVERSIDADE SEVERINO SOMBRA; BIANCA BRAZ MATTOS, CNPS; FABIANO DE CARVALHO BALIEIRO, CNPS. |
|
Título: |
Evaluation of dispersant solutions for the determination of available phosphorus in particule size fractions in a Brazilian Sandy Soil. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
In: WORLD CONGRESS OF SOIL SCIENCE, 21., 2018, Rio de Janeiro. Soil science: beyond food and fuel: proceedings... Viçosa, MG: SBCS, 2019. v. 2, p. 415. WCSS 2018. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The objective of this work was to evaluate different dispersant solutions for subsequent soil fractionation, and analysis of available P in particulate organic matter and in the heavy fraction. |
|
Thesagro: |
Fósforo; Matéria Orgânica; Solo. |
|
Thesaurus NAL: |
Phosphorus; Soil organic matter. |
|
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/205396/1/Evaluation-of-dispersant-solutions-for-the-determination-of-available-phosphorus-in-particule-size-fractions-in-a-Brazilian-Sandy-Soil-2019.pdf
|
|
Marc: |
LEADER 00971nam a2200205 a 4500
001 2115032
005 2020-01-03
008 2019 bl uuuu u00u1 u #d
100 1 $aCAMPOS, D. V. B. de
245 $aEvaluation of dispersant solutions for the determination of available phosphorus in particule size fractions in a Brazilian Sandy Soil.$h[electronic resource]
260 $aIn: WORLD CONGRESS OF SOIL SCIENCE, 21., 2018, Rio de Janeiro. Soil science: beyond food and fuel: proceedings... Viçosa, MG: SBCS, 2019. v. 2, p. 415. WCSS 2018.$c2018
520 $aThe objective of this work was to evaluate different dispersant solutions for subsequent soil fractionation, and analysis of available P in particulate organic matter and in the heavy fraction.
650 $aPhosphorus
650 $aSoil organic matter
650 $aFósforo
650 $aMatéria Orgânica
650 $aSolo
700 1 $aCHAVES, L. da S.
700 1 $aMATTOS, B. B.
700 1 $aBALIEIRO, F. de C.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Solos (CNPS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
