|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agrobiologia. |
|
Data corrente: |
18/01/2001 |
|
Data da última atualização: |
18/01/2001 |
|
Autoria: |
FRUHLING, M.; HOHNJEC, N.; SCHRODER, G.; KUSTER, H.; PUHLER, A.; PERLICK, A. M. |
|
Título: |
Genomic organization and expression properties of the VfENOD5 gene from broad bean (Vicia faba L.). |
|
Ano de publicação: |
2000 |
|
Fonte/Imprenta: |
Plant Science, Ireland, v. 155, p. 169-178, 2000. |
|
Idioma: |
Inglês |
|
Conteúdo: |
A full-length cDNA encoding the broad bean (Vicia faba L.) early nodulin VIENOD5 was isolated from a nodule cDNA library. In addition to the ENOD5 homologues from other legumes the derived VfENOD5 amino acid sequence also displayed homologies to the phytocyanin-related nodulins GmENOD55-2, MtENOD16, and MtENOD20. A close inspection of the ENOD5 proteins from broad bean, pea and vetch indicated that all these nodulins possess a putative C-terminal GPI-anchor signal sequence. This novel finding supports the bypothesis that ENOD5 is an arabinogalactan protein. Tissue print hybridizations revealed that the broad bean ENOD5 gene was not only expressed in the central tissue of root nodules. In contrast to other legumes hybridizing transcripts were also be detected in a narrow zone within the peripheral nodule tissues. Sequence analysis of a genomic clone indicated the presence of a single intron interrupting the VfENOD5 coding region at a position precisely corresponding to the MtENOD16 and MtENOD20 introns. |
|
Palavras-Chave: |
Feijão faba; Pea. |
|
Thesagro: |
Ervilha; Vicia Faba. |
|
Thesaurus Nal: |
faba beans. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01657naa a2200241 a 4500
001 1621192
005 2001-01-18
008 2000 bl --- 0-- u #d
100 1 $aFRUHLING, M.
245 $aGenomic organization and expression properties of the VfENOD5 gene from broad bean (Vicia faba L.).
260 $c2000
520 $aA full-length cDNA encoding the broad bean (Vicia faba L.) early nodulin VIENOD5 was isolated from a nodule cDNA library. In addition to the ENOD5 homologues from other legumes the derived VfENOD5 amino acid sequence also displayed homologies to the phytocyanin-related nodulins GmENOD55-2, MtENOD16, and MtENOD20. A close inspection of the ENOD5 proteins from broad bean, pea and vetch indicated that all these nodulins possess a putative C-terminal GPI-anchor signal sequence. This novel finding supports the bypothesis that ENOD5 is an arabinogalactan protein. Tissue print hybridizations revealed that the broad bean ENOD5 gene was not only expressed in the central tissue of root nodules. In contrast to other legumes hybridizing transcripts were also be detected in a narrow zone within the peripheral nodule tissues. Sequence analysis of a genomic clone indicated the presence of a single intron interrupting the VfENOD5 coding region at a position precisely corresponding to the MtENOD16 and MtENOD20 introns.
650 $afaba beans
650 $aErvilha
650 $aVicia Faba
653 $aFeijão faba
653 $aPea
700 1 $aHOHNJEC, N.
700 1 $aSCHRODER, G.
700 1 $aKUSTER, H.
700 1 $aPUHLER, A.
700 1 $aPERLICK, A. M.
773 $tPlant Science, Ireland$gv. 155, p. 169-178, 2000.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
